Voltage- and Ca2+-Dependent Burst Generation in Neuroendocrine Dahlgren Cells in the Teleost Platichthys flesus
| العنوان: | Voltage- and Ca2+-Dependent Burst Generation in Neuroendocrine Dahlgren Cells in the Teleost Platichthys flesus |
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| المؤلفون: | Weiqun Lu, C. S. Bauer, MJ Brierley, Catherine R. McCrohan, Daniela Riccardi, Richard J. Balment |
| المصدر: | Journal of Neuroendocrinology. 16:832-841 |
| بيانات النشر: | Wiley, 2004. |
| سنة النشر: | 2004 |
| مصطلحات موضوعية: | medicine.medical_specialty, Patch-Clamp Techniques, Calcium Channels, L-Type, Endocrinology, Diabetes and Metabolism, Flounder, Tetrodotoxin, In Vitro Techniques, Cation Channel Blocker, Apamin, Membrane Potentials, Potassium Channels, Calcium-Activated, Cellular and Molecular Neuroscience, Bursting, chemistry.chemical_compound, Endocrinology, Nifedipine, Internal medicine, Potassium Channel Blockers, medicine, Animals, Channel blocker, Calcium Signaling, Patch clamp, Neurons, Voltage-dependent calcium channel, Endocrine and Autonomic Systems, Cell Membrane, Potassium channel blocker, 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester, Tetraethylammonium Compounds, Calcium Channel Blockers, Neurosecretory Systems, Electrophysiology, chemistry, Sodium Channel Blockers, medicine.drug |
| الوصف: | The neuroendocrine Type 1 Dahlgren cells of the caudal neurosecretory system of the flounder display characteristic bursting activity, which may increase secretion efficiency. The firing activity pattern in these cells was voltage-dependent; when progressively depolarized, cells moved from silent (approximately -70 mV), through bursting and phasic to tonic firing (< -65 mV). Brief (10 s) evoked bursts of spikes were followed by a slow after-depolarization (ADP; amplitude up to 10 mV, duration 10-200 s), which was also voltage-dependent and could trigger a prolonged burst. The ADP was significantly reduced in the absence of external Ca(2+) ions or the presence of the L-type Ca(2+) channel blocker, nifedipine. BayK 8644 (which increases L-type channel open times) significantly increased ADP duration, whereas the Ca(2+)-activated nonselective cation channel blocker, flufenamic acid, had no effect. Pharmacological blockade of Ca(2+)-activated K(+) channels, using apamin and charybdotoxin, increased the duration of both ADP and evoked bursts. However, action potential waveform was unaffected by either apamin/charybdotoxin, nifedipine, BayK 8644 or removal of external Ca(2+). The short duration (approximately 100 ms), hyperpolarization-activated, postspike depolarizing afterpotentials (DAP), were significantly reduced by nifedipine. We propose that long duration ADPs underlie bursts and that short duration DAPs play a role in modulation of spike frequency. |
| تدمد: | 1365-2826 0953-8194 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::da2615452e64661e165449b4173cba93Test https://doi.org/10.1111/j.1365-2826.2004.01238.xTest |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....da2615452e64661e165449b4173cba93 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13652826 09538194 |
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