Identification and Characterization of Three New Antimicrobial Peptides from the Marine Mollusk Nerita versicolor (Gmelin, 1791)
| العنوان: | Identification and Characterization of Three New Antimicrobial Peptides from the Marine Mollusk Nerita versicolor (Gmelin, 1791) |
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| المؤلفون: | Armando Rodriguez, Ernesto M. Martell-Huguet, Melaine González-García, Daniel Alpízar-Pedraza, Annia Alba, Antonio A. Vazquez, Mark Grieshober, Barbara Spellerberg, Steffen Stenger, Jan Münch, Ann-Kathrin Kissmann, Frank Rosenau, Ludger A. Wessjohann, Sebastian Wiese, Ludger Ständker, Anselmo J. Otero-Gonzalez |
| المصدر: | International Journal of Molecular Sciences Volume 24 Issue 4 Pages: 3852 |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Inorganic Chemistry, marine invertebrates, antimicrobial peptides, Organic Chemistry, bioinformatical prediction, General Medicine, Physical and Theoretical Chemistry, antibiotic alternatives, Molecular Biology, Spectroscopy, Catalysis, Computer Science Applications, mass spectrometry |
| الوصف: | Mollusks have been widely investigated for antimicrobial peptides because their humoral defense against pathogens is mainly based on these small biomolecules. In this report, we describe the identification of three novel antimicrobial peptides from the marine mollusk Nerita versicolor. A pool of N. versicolor peptides was analyzed with nanoLC-ESI-MS-MS technology, and three potential antimicrobial peptides (Nv-p1, Nv-p2 and Nv-p3) were identified with bioinformatical predictions and selected for chemical synthesis and evaluation of their biological activity. Database searches showed that two of them show partial identity to histone H4 peptide fragments from other invertebrate species. Structural predictions revealed that they all adopt a random coil structure even when placed near a lipid bilayer patch. Nv-p1, Nv-p2 and Nv-p3 exhibited activity against Pseudomonas aeruginosa. The most active peptide was Nv-p3 with an inhibitory activity starting at 1.5 µg/mL in the radial diffusion assays. The peptides were ineffective against Klebsiella pneumoniae, Listeria monocytogenes and Mycobacterium tuberculosis. On the other hand, these peptides demonstrated effective antibiofilm action against Candida albicans, Candida parapsilosis and Candida auris but not against the planktonic cells. None of the peptides had significant toxicity on primary human macrophages and fetal lung fibroblasts at effective antimicrobial concentrations. Our results indicate that N. versicolor-derived peptides represent new AMP sequences and have the potential to be optimized and developed into antibiotic alternatives against bacterial and fungal infections. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1422-0067 |
| DOI: | 10.3390/ijms24043852 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::21524c53001c0609d44b381d07fff2c2Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....21524c53001c0609d44b381d07fff2c2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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| DOI: | 10.3390/ijms24043852 |