ALCAM (CD166) is involved in extravasation of monocytes rather than T cells across the blood–brain barrier
| العنوان: | ALCAM (CD166) is involved in extravasation of monocytes rather than T cells across the blood–brain barrier |
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| المؤلفون: | Alexandres Prat, Michael Abadier, Marc-André Lécuyer, Urban Deutsch, Ana Bélen Garcia Martin, Christof B. Wyss, Britta Engelhardt, Gaby Enzmann, Fabien Gosselet, Ruth Lyck, Nicole Schaeren-Wiemers, Joshua A. Weiner, Maria Rosito, Federica Sallusto, Christoph Matti, Thomas Zeis, Laure Michel |
| المساهمون: | Theodor Kocher Institute, University of Bern, Centre de Recherche du Centre Hospitalier de l’Université de Montréal (CR CHUM), Centre Hospitalier de l'Université de Montréal (CHUM), Université de Montréal (UdeM)-Université de Montréal (UdeM), University Hospital Basel [Basel], Institute for Research in Biomedicine, Laboratoire de la Barrière Hémato-Encéphalique (LBHE), Université d'Artois (UA), University of Iowa [Iowa City] |
| المصدر: | Journal of Cerebral Blood Flow and Metabolism Journal of Cerebral Blood Flow and Metabolism, Nature Publishing Group, 2017, 37 (8), pp.2894-2909. ⟨10.1177/0271678X16678639⟩ |
| بيانات النشر: | SAGE Publications Ltd, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Pathology, Leukocyte migration, alcam, blood–brain barrier, immune cell extravasation, multiple sclerosis, neuroinflammation, animals, antigens, cd, blood-brain barrier, cell adhesion molecules, neuronal, cells, cultured, encephalomyelitis, autoimmune, experimental, endothelial cells, endothelium, vascular, fetal proteins, humans, mice, inbred c57bl, knockout, monocytes, t-lymphocytes, transendothelial and transepithelial migration, [SDV]Life Sciences [q-bio], 0302 clinical medicine, 610 Medicine & health, T cell extravasation, Cells, Cultured, Mice, Knockout, Experimental autoimmune encephalomyelitis, Activated-Leukocyte Cell Adhesion Molecule, Extravasation, Cell biology, medicine.anatomical_structure, Neurology, cardiovascular system, Cardiology and Cardiovascular Medicine, medicine.medical_specialty, Encephalomyelitis, Autoimmune, Experimental, Cell Adhesion Molecules, Neuronal, Biology, Blood–brain barrier, 03 medical and health sciences, Antigens, CD, medicine, ALCAM, Neuroinflammation, Original Articles, medicine.disease, Mice, Inbred C57BL, 030104 developmental biology, Neurology (clinical), Endothelium, Vascular, 030217 neurology & neurosurgery |
| الوصف: | Activated leukocyte cell adhesion molecule (ALCAM) has been proposed to mediate leukocyte migration across the blood–brain barrier (BBB) in multiple sclerosis or experimental autoimmune encephalomyelitis (EAE). Here, we confirmed vascular ALCAM expression in human brain tissue samples in situ and on two different human in vitro BBB models. Antibody-mediated inhibition of ALCAM reduced diapedesis of human CD4+ Th1 but not of Th17 cells across the human BBB in vitro. In accordance to human Th1 cells, mouse Th1 cells showed reduced diapedesis across an ALCAM−/− in vitro BBB model under static but no longer under flow conditions. In contrast to the limited role of ALCAM in T cell extravasation across the BBB, we found a contribution of ALCAM to rolling, adhesion, and diapedesis of human CD14+ monocytes across the human BBB under flow and static conditions. Taken together, our study highlights the potential differences in the CNS expression of ALCAM in mouse and human and supports a prominent role for ALCAM in the multi-step extravasation of monocytes across the BBB. |
| اللغة: | English |
| تدمد: | 0271-678X 1559-7016 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3fbcebcff048c33494fce282ab9d3b8dTest http://hdl.handle.net/11573/1608970Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3fbcebcff048c33494fce282ab9d3b8d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 0271678X 15597016 |
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