The MyD88+ phenotype is an adverse prognostic factor in epithelial ovarian cancer
| العنوان: | The MyD88+ phenotype is an adverse prognostic factor in epithelial ovarian cancer |
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| المؤلفون: | John J. O'Leary, Dearbhaile M. O'Donnell, Sharon O'Toole, Luke A. J. O'Neill, Amanda McCann, Brian Flood, Kathryn Haley, Noreen Gleeson, Ciara Murphy, Bryan T. Hennessy, Stephen P. Finn, Stephen R. Pennington, Cathy D. Spillane, Michael F Gallagher, Britta K. Stordal, Cara Martin, Aoife A. Cooke, Kenneth J. O'Byrne, Orla Sheils, Mark Bates, Brendan Ffrench, Katie M. Costello, Niamh Kernan, Jacqui Barry-O'Crowley, Tom D'Arcy, Charles d’Adhemar, Paul Smyth |
| المصدر: | PLoS ONE, Vol 9, Iss 6, p e100816 (2014) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Cellular differentiation, lcsh:Medicine, Autoimmunity, Carcinoma, Ovarian Epithelial, Biochemistry, Nucleic Acids, Molecular Cell Biology, Neoplasms, Glandular and Epithelial, RNA, Small Interfering, lcsh:Science, Ovarian Neoplasms, Multidisciplinary, Genomics, Middle Aged, Prognosis, Immunohistochemistry, Functional Genomics, 3. Good health, Gene Expression Regulation, Neoplastic, Phenotype, Neoplastic Stem Cells, Female, Anatomy, Stem cell, Transcriptome Analysis, Research Article, Signal Transduction, Histology, Genotype, Paclitaxel, Immunology, Biology, Cancer stem cell, Cell Line, Tumor, microRNA, Genetics, medicine, Carcinoma, Humans, Aged, lcsh:R, Biology and Life Sciences, Computational Biology, Cancer, Cell Biology, Genome Analysis, medicine.disease, Antineoplastic Agents, Phytogenic, Survival Analysis, Cystadenocarcinoma, Serous, Toll-Like Receptor 4, MicroRNAs, Drug Resistance, Neoplasm, Myeloid Differentiation Factor 88, Cancer cell, Cancer research, RNA, lcsh:Q, Genome Expression Analysis, Ovarian cancer |
| الوصف: | The prognosis of epithelial ovarian cancer is poor in part due to the high frequency of chemoresistance. Recent evidence points to the Toll-like receptor-4 (TLR4), and particularly its adaptor protein MyD88, as one potential mediator of this resistance. This study aims to provide further evidence that MyD88 positive cancer cells are clinically significant, stem-like and reproducibly detectable for the purposes of prognostic stratification. Expression of TLR4 and MyD88 was assessed immunohistochemically in 198 paraffin-embedded ovarian tissues and in an embryonal carcinoma model of cancer stemness. In parallel, expression of TLR4 and MyD88 mRNA and regulatory microRNAs (miR-21 and miR-146a) was assessed, as well as in a series of chemosensitive and resistant cancer cells lines. Functional analysis of the pathway was assessed in chemoresistant SKOV-3 ovarian cancer cells. TLR4 and MyD88 expression can be reproducibly assessed via immunohistochemistry using a semi-quantitative scoring system. TLR4 expression was present in all ovarian epithelium (normal and neoplastic), whereas MyD88 was restricted to neoplastic cells, independent of tumour grade and associated with reduced progression-free and overall survival, in an immunohistological specific subset of serous carcinomas, p |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::054dd4c375238746aeb819e7402f12b7Test http://europepmc.org/articles/PMC4076208?pdf=renderTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....054dd4c375238746aeb819e7402f12b7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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