Expression of the HOXA gene family and its relationship to prognosis and immune infiltrates in cervical cancer
| العنوان: | Expression of the HOXA gene family and its relationship to prognosis and immune infiltrates in cervical cancer |
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| المؤلفون: | Junli Zhang, Fenfen Ge, Yingying Fan, Yingying Zhu, Weiwei Tie |
| المصدر: | Journal of Clinical Laboratory Analysis |
| بيانات النشر: | Wiley, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Microbiology (medical), cervical cancer, diagnosis, Clinical Biochemistry, Uterine Cervical Neoplasms, Biology, HOXA Gene Family, Immune system, Biomarkers, Tumor, medicine, Humans, Immunology and Allergy, Gene family, Clinical significance, RNA-Seq, Gene, Research Articles, ImmuCellAI, Homeodomain Proteins, Cervical cancer, Biochemistry (medical), Public Health, Environmental and Occupational Health, Hematology, Prognosis, medicine.disease, HOXA family, Medical Laboratory Technology, HOXA1 Gene, Cancer research, Homeobox, Female, immunity biomarkers, Transcriptome, Research Article |
| الوصف: | Background The homeobox A cluster (HOXA) gene family is participated in multiple biological functions in human cancers. To date, little is known about the expression profile and clinical significance of HOXA genes in cervical cancer. Methods We downloaded RNASeq data of cervical cancer from The Cancer Genome Atlas (TCGA) database. The difference in HOXA family expression was analyzed using independent samples t test. Cox proportional hazard regression analysis was used to assess the effect of HOXA family expression on survival, and a nomogram predicting survival was generated. We assessed the infiltration difference in immune cells and expression difference of immunity biomarkers between two groups with different expression level of HOXA genes through Immune Cell Abundance Identifier (ImmuCellAI) and independent samples t test, respectively. Results Our results showed that the HOXA1 gene was upregulated, while the HOXA10 and HOXA11 were downregulated in cervical cancer. Downregulation of HOXA1 was related to a poor outcome for cervical cancer patient. We also identified a significantly increased abundance of T helper 2 cells (Th2) and higher expression of PD‐L1 in cervical cancer patients with lower expression of HOXA10 and HOXA11. The gene set enrichment analysis (GSEA) results indicated that HOXA1 and HOXA11 were involved in immune responses pathways and participated in the activation of a variety of classic signaling pathways related to the progression of human cancer. Conclusion This study comprehensively analyzed different HOXA genes applying public database to determine their expression patterns, potential diagnostic, prognostic, and treatment values in cervical cancer. Our study comprehensively analyzed different HOXA genes applying public database to determine their expression patterns, potential diagnostic, prognostic, and treatment values in cervical cancer. Firstly, we compared the expression of HOXA members in cervical cancer than normal controls, and results showed only three HOXA members with statistical significance (HOXA1, HOXA10, and HOXA11). Then, the prognostic potential was assessed by the following steps: (1) Kaplan‐Meier plot showed low expression of seven HOXA members was correlated with favor overall survival of cervical cancer (including HOXA1, HOXA2, HOAX3, HOXA4, HOXA5, HOXA6, and HOXA9). (2) Univariate Cox proportional hazards regression analysis confirmed the prognostic value of HOXA1, HOXA2, HOXA3, and HOXA4. (3) Multivariate Cox proportional hazards regression analysis determined the prognostic value of HOXA1 and HOXA3. Eventually, the HOXA1 could be served as an independent prognostic biomarker for poor outcome of cervical cancer. Subsequently, a significantly increased abundance of T helper 2 cells (Th2) and higher expression of PD‐L1 were observed in cervical cancer patients with lower expression of HOXA10 and HOXA11, indicative of the fact that HOXA10 and HOXA11 could be served as biomarkers of response to immunotherapy. |
| تدمد: | 1098-2825 0887-8013 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad31015d66155ebd2840e70cba88d8eaTest https://doi.org/10.1002/jcla.24015Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ad31015d66155ebd2840e70cba88d8ea |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10982825 08878013 |
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