دورية أكاديمية
Ki-67 Expression in Breast Cancer Tissue Microarrays
| العنوان: | Ki-67 Expression in Breast Cancer Tissue Microarrays |
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| المؤلفون: | Khoury, T, Zirpoli, G, Cohen, S.M, Geradts, J, Omilian, A, Davis, W, Bshara, W, Miller, R, Mathews, M.M, Troester, M, Palmer, J.R, Ambrosone, C.B |
| المصدر: | American Journal of Clinical Pathology, 148(2) |
| سنة النشر: | 2017 |
| المجموعة: | Carolina Digital Repository (UNC - University of North Carolina) |
| مصطلحات موضوعية: | major clinical study, antigen expression, estrogen receptor, priority journal, Tissue Array Analysis, middle aged, human, biological marker, correlation analysis, cancer classification, diagnostic test accuracy study, data base, tumor cell, intraductal carcinoma, full section, tissue fixation, breast cancer, sensitivity and specificity, epidermal growth factor receptor 2, tissue microarray, immunohistochemistry, adult, tumor marker, medical society, procedures, staining, female, diagnostic accuracy, breast biopsy, Biomarkers |
| الوصف: | Objectives Ki-67 has been proposed to be used as a surrogate marker to differentiate luminal breast carcinomas (BCs). The purpose of this study was to determine the utility of and best approaches for using tissue microarrays (TMAs) and Ki-67 staining to distinguish luminal subtypes in large epidemiology studies of luminal/human epidermal growth factor receptor 2 (HER2)-negative BC. Methods Full-section and TMA (three 0.6-mm cores and two 1.0-mm cores) slides of 109 cases were stained with Ki-67 antibody. We assessed two ways of collapsing TMA cores: a weighted approach and mitotically active approach. Results For cases with at least a single 0.6-mm TMA core (n = 107), 16% were misclassified using a mitotically active approach and 11% using a weighted approach. For cases with at least a single 1.0-mm TMA core (n = 101), 5% were misclassified using either approach. For the 0.6-mm core group, there were 33.3% discordant cases. The number of discordant cases increased from 18% in the group of two cores to 40% in the group of three cores (P =.039). Conclusions Ki-67 tumor heterogeneity was common in luminal/HER2- BC. Using a weighted approach was better than using a mitotically active approach for core to case collapsing. At least a single 1.0-mm core or three 0.6-mm cores are required when designing a study using TMA. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://doi.org/10.17615/s59a-5b63Test; https://cdr.lib.unc.edu/downloads/tx31qv971?file=thumbnailTest; https://cdr.lib.unc.edu/downloads/tx31qv971Test |
| DOI: | 10.17615/s59a-5b63 |
| الإتاحة: | https://doi.org/10.17615/s59a-5b63Test https://cdr.lib.unc.edu/downloads/tx31qv971?file=thumbnailTest https://cdr.lib.unc.edu/downloads/tx31qv971Test |
| رقم الانضمام: | edsbas.B27B93D5 |
| قاعدة البيانات: | BASE |
| DOI: | 10.17615/s59a-5b63 |
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