المؤلفون: Byung-Kwon Lee, Chun Sung Kim, Do Kyung Kim, Heung-Joong Kim, Hitoshi Endou, Hong Sung Chun, In-Sung Moon, Joo-Cheol Park, Joong-Ki Kook, Ju-Hyun Park, Sook-Young Lee, Woo-Cheol Shin, Yoshikatsu Kanai

المصدر: Biological and Pharmaceutical Bulletin. 2010, 33(7):1117

المؤلفون: Dae-San Go, Jong-Tae Park, Seul-Ah Lee, Chun Sung Kim, Jeongsun Kim, Hong Sung Chun, Heung-Joong Kim, Do Kyung Kim, Jae-Sung Kim, Sun Young Park, Sun-Kyoung Yu

المصدر: Journal of Bioscience and Bioengineering. 120:351-358

مصطلحات موضوعية: Untranslated region, Population, Down-Regulation, Apoptosis, Bioengineering, Biology, Transfection, Applied Microbiology and Biotechnology, Cell Line, Tumor, Gene expression, Humans, Genes, Tumor Suppressor, RNA, Messenger, education, 3' Untranslated Regions, Oligonucleotide Array Sequence Analysis, Proto-Oncogene Proteins c-yes, education.field_of_study, Dose-Response Relationship, Drug, Kinase, Molecular biology, Gene Expression Regulation, Neoplastic, MicroRNAs, Cancer cell, Mouth Neoplasms, miR-203, Biotechnology

الوصف: The purpose of this study was to elucidate the molecular mechanisms of microRNA-203 (miR-203) as a tumor suppressor in KB human oral cancer cells. MicroRNA microarray results showed that the expression of miR-203 was significantly down-regulated in KB cells compared with normal human oral keratinocytes. The viability of KB cells was decreased by miR-203 in the time- and dose-dependent manners. In addition, over-expressed miR-203 not only increased the nuclear condensation but also significantly increased the apoptotic population of KB cells. These results indicated that the over-expression of miR-203 induced apoptosis of KB cells. Furthermore, the target gene array analyses revealed that the expression of Yes-1, a member of the Src family kinases (SFKs), was significantly down-regulated by miR-203 in KB cells. Moreover, both the mRNA and protein levels of Yes-1 were strongly reduced in KB cells transfected with miR-203. Therefore, these results indicated that Yes-1 is predicted to be a potential target gene of miR-203. Through a luciferase activity assay, miR-203 was confirmed to directly targets the Yes-1 3' untranslated region (UTR) to suppress gene expression. Therefore, our findings indicate that miR-203 induces the apoptosis of KB cells by directly targeting Yes-1, suggesting its application in anti-cancer therapeutics.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8406b350d832a09b4f6b51b9c43f65ebTest
https://doi.org/10.1016/j.jbiosc.2015.02.002Test

المؤلفون: Su-Gwan Kim, Jin-Soo Kim, Heung-Joong Kim, Seul Ah Lee, Dae-Woong Yun, Chun Sung Kim, Michael F. Wempe, Min-Gyeong Park, Yoshikatsu Kanai, Do Kyung Kim, Jae-Sung Kim, Hong Sung Chun, Sun-Kyoung Yu, Hitoshi Endou, Ji-Su Oh, Mi-Ra Park

المصدر: Journal of Pharmacological Sciences, Vol 124, Iss 2, Pp 208-217 (2014)

مصطلحات موضوعية: Fusion Regulatory Protein 1, Heavy Chain, Population, Antineoplastic Agents, Apoptosis, Large Neutral Amino Acid-Transporter 1, Leucine, Tumor Cells, Cultured, Glutamate aspartate transporter, Humans, Amino acid transporter, education, Caspase, Pharmacology, Benzoxazoles, education.field_of_study, biology, Cell growth, lcsh:RM1-950, Molecular biology, Up-Regulation, lcsh:Therapeutics. Pharmacology, Biochemistry, Caspases, Amino Acid Transport System L, Cancer cell, biology.protein, Tyrosine, Molecular Medicine, Mouth Neoplasms, Intracellular

الوصف: Compared to most normal cells that express L-type amino acid transporter 2, L-type amino acid transporter 1 is highly expressed in cancer cells and presumed to support their elevated growth and proliferation. This study examined JPH203, a potent and selective L-type amino acid transporter 1 inhibitor, and its ability to suppress YD-38 human oral cancer cell growth. The YD-38 cells express L-type amino acid transporter 1 with its associating protein 4F2 heavy chain, but not L-type amino acid transporter 2. JPH203 and BCH, a non-selective L-type amino acid transporter inhibitor, completely inhibited l-leucine uptake in YD-38 cells. As expected, the intrinsic affinity of JPH203 to inhibit l-leucine uptake was far more efficient than BCH. Likewise, JPH203 and BCH inhibited YD-38 cell growth, with JPH203 being superior to BCH. JPH203 up-regulated the population of apoptotic YD-38 cells through the activation of apoptotic factors, including caspases and PARP. These results suggest that the inhibition of L-type amino acid transporter 1 activity via JPH203, which may act as a potential novel anti–oral-cancer agent, leads to apoptosis by inducing the intracellular depletion of the neutral amino acids essential for cancer cell growth in YD-38 human oral cancer cells. Keywords:: JPH203, L-type amino acid transporter 1, apoptosis, oral cancer cell, anti-cancer therapy

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5baf95cd0514ad8f2dd14cb7ed03f133Test
https://doi.org/10.1254/jphs.13154fpTest

المؤلفون: Hyun-Sang Jeon, Heung-Joong Kim, Do Kyung Kim, Myoung-Hwa Lee, Hong Sung Chun, Sook-Young Lee, Su-Gwan Kim, Euteum Park, Myung-Hun Jo, Sun-Kyoung Yu, Chun Sung Kim

المصدر: Journal of the Korean Society for Applied Biological Chemistry. 55:451-456

مصطلحات موضوعية: chemistry.chemical_compound, Programmed cell death, chemistry, Cell growth, Apoptosis, Organic Chemistry, Cancer cell, Curcumin, Potency, Pharmacology, Cleavage (embryo), Apoptosis induction, General Biochemistry, Genetics and Molecular Biology

الوصف: Effects of diphenyl difluoroketone (EF-24) and curcumin on cell growth and apoptosis induction in KB human oral cancer cells were examined. EF-24 and curcumin inhibited the growth of KB cells in a dose-dependent manner, and the potency of EF-24 was 30 times greater than that of curcumin. Treatment with EF-24 or curcumin resulted in nuclear condensation and fragmentation. EF-24 and curcumin promoted the proteolytic cleavage of procaspases-3, -7, and -9. Activities of caspases-3 and -7 were detected in living KB cells treated with EF-24 or curcumin. These results suggest that EF-24 and curcumin inhibit cell proliferation and induce apoptosis in KB human oral cancer cells, and have potential properties for development of anti oral cancer drug.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::237cab66e953402d67a667248e60e3a7Test
https://doi.org/10.1007/s13765-012-1168-8Test

المؤلفون: Seong Hoon Kim, Hong Sung Chun, Myoung Hwa Lee, Eu Teum Park, Joong-Ki Kook, Do Kyung Kim, Hye Ryun Kim, Heung Joong Kim, Su Gwan Kim, Sook-Young Lee, Chun Sung Kim, Sun Kyoung Yu

المصدر: Journal of the Korean Society for Applied Biological Chemistry. 54:966-971

مصطلحات موضوعية: chemistry.chemical_classification, Programmed cell death, endocrine system diseases, Chemistry, Cell growth, Phytoalexin, Organic Chemistry, food and beverages, Resveratrol, Molecular biology, General Biochemistry, Genetics and Molecular Biology, chemistry.chemical_compound, Apoptosis, Cancer cell, DNA fragmentation, DNA

الوصف: Resveratrol (trans-3,4′s,5,-trihydroxystilbene), a phytoalexin present in grape skin and red wine, suppresses many types of cancers by regulating cell proliferation and apoptosis through a variety of mechanisms. However, the effects of resveratrol on oral cancer are not completely understood. Thus, effects of resveratrol on cell growth and apoptosis induction were examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, DNA fragmentation, immunoblotting, and determination of caspase activation in KB human oral cancer cells. Treatment with resveratrol induced inhibition of cell growth depending on the resveratrol treatment time and concentration in KB cells. Treatment with resveratrol induced DNA ladder formation in KB cells and promoted proteolytic cleavage of procaspase-3 and procaspase-7 with increases in the amount of cleaved caspases-3 and -7. Proteolytic processing of caspase-9 in KB cells was increased by resveratrol treatment. Activation of caspase-3/-7 was detected in living KB cells by fluorescence microscopy. These results suggest that the resveratrol can suppress cell growth and induce cell apoptosis in KB human oral cancer cells, and may have potential as an anti-cancer drug.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::0a388a5ddbdddc91a25d1610472abf3eTest
https://doi.org/10.1007/bf03253187Test

المؤلفون: Yoshikatsu Kanai, Joo-Cheol Park, Woo-Cheol Shin, Ju-Hyun Park, Hitoshi Endou, Chun Sung Kim, In-Sung Moon, Byung-Kwon Lee, Sook-Young Lee, Do Kyung Kim, Hong Sung Chun, Joong-Ki Kook, Heung-Joong Kim

المصدر: Biological and Pharmaceutical Bulletin. 33:1117-1121

مصطلحات موضوعية: Pharmacology, Cell cycle checkpoint, Amino Acid Transport Systems, biology, Cell growth, Blotting, Western, Pharmaceutical Science, Cell Cycle Proteins, General Medicine, Cell cycle, Molecular biology, chemistry.chemical_compound, chemistry, Cell culture, Cyclin-dependent kinase, Cell Line, Tumor, Cancer cell, biology.protein, Humans, Immunoprecipitation, Mouth Neoplasms, Cyclin-dependent kinase 6, Growth inhibition

الوصف: The purpose of this study was to examine the effect of 2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid (BCH), an inhibitor of L-type amino acid transporters, on the cell growth suppression in KB human oral cancer cells and to study the roles of cell cycle regulatory factors in the BCH-induced growth inhibition. The effect of BCH on cell growth suppression and the influence of BCH to cell cycle regulatory factors in KB cell growth inhibition were examined using cell cycle analysis, immunoblotting and immunoprecipitation. The BCH treatment induced cell cycle arrest at G1 phase in KB cells. The expression of cyclin D3 was remarkably decreased by BCH treatment. The BCH inhibited the expression of cyclin-dependent protein kinase 6 (CDK6) in a time-dependent manner. In addition, the expression of CDK inhibitor p27 was increased by BCH treatment in KB cells, but not CDK inhibitors p21 and p15. These results suggest that, in KB cells, the inhibition of LAT1 by BCH causes cell cycle arrest at G1 phase by inhibiting cyclin D3-CDK6 complex whereas increasing expression of a CDK inhibitor p27.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4bc49ba1fe90fef73d57c38c06328250Test
https://doi.org/10.1248/bpb.33.1117Test

المؤلفون: Seoul Lee, Heung-Joong Kim, Do Kyung Kim, Chong Heon Lee, Bong Kyu Choi, Hyun Kuk Kim, Jung Hoon Yoon, Yoshikatsu Kanai, Sang Gun Ahn, In Jin Kim, Hitoshi Endou, Moon Jin Jeong, Kyu Yong Jung, Jong-Keun Kim, Soo Ah Kim

المصدر: Cancer Letters. 222:237-245

مصطلحات موضوعية: Keratinocytes, chemistry.chemical_classification, Cancer Research, System L, Gene Expression Profiling, Protein subunit, Carcinoma, Transporter, Biology, Polymerase Chain Reaction, Amino acid, Gene expression profiling, Oncology, Biochemistry, chemistry, Epidermoid carcinoma, Amino Acid Transport System L, Cancer cell, Humans, Mouth Neoplasms, Amino acid transporter

الوصف: Previously, we reported the expression and function of system L amino acid transporter in KB human oral epidermoid carcinoma cells. In the present study, therefore, we investigated the expression and function of system L amino acid transporter in human normal oral keratinocytes (HNOK) and compared the expressions and functions of system L amino acid transporters in HNOK and KB cells. The HNOK expressed L-type amino acid transporter 1 (LAT1) and L-type amino acid transporter 2 (LAT2) with their subunit 4F2hc in the plasma membrane but the expression of LAT1 was very weak, which is in contrast to the KB cells expressing LAT1 but not LAT2 with the 4F2hc in the plasma membrane. The [14C] L-leucine uptake by HNOK, as well as KB cells, was inhibited by the system L selective inhibitor BCH. The majority of [14C] L-leucine uptake was, therefore, mainly mediated by LAT2 in the HNOK and by LAT1 in the KB cells. These results suggest that the transport of neutral amino acids including several essential amino acids into the HNOK and KB cells are mainly mediated by LAT2 and LAT1, respectively. The specific inhibition of LAT1 in oral cancer cells could be a new rationale for anti-cancer therapy.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7d3ae8549a53792f3bc2d32d25f640c9Test
https://doi.org/10.1016/j.canlet.2004.09.040Test

المؤلفون: Hong Sung Chun, Mi Suk Choi, Hoon Ahn, Heung-Joong Kim, Sang Joon Yun, Byung-Ock Kim, Sung-Min Moon, Su-Gwan Kim, Bo-Ram Park, Chun Sung Kim, Do Kyung Kim, Sook-Young Lee, Joong-Ki Kook

المصدر: Pharmaceutical biology. 51(11)

مصطلحات موضوعية: Saussurea, Programmed cell death, Time Factors, Cell Survival, Pharmaceutical Science, Apoptosis, DNA Fragmentation, Pharmacology, Plant Roots, KB Cells, Drug Discovery, Medicine, Humans, Viability assay, Saussurea lappa, Cell Proliferation, bcl-2-Associated X Protein, Mouth neoplasm, Caspase 7, Plants, Medicinal, Traditional medicine, Dose-Response Relationship, Drug, business.industry, Cell growth, Caspase 3, Plant Extracts, Methanol, General Medicine, Antineoplastic Agents, Phytogenic, Caspase 9, Complementary and alternative medicine, Proto-Oncogene Proteins c-bcl-2, Cancer cell, Solvents, Molecular Medicine, DNA fragmentation, Mouth Neoplasms, Poly(ADP-ribose) Polymerases, business, Phytotherapy, Signal Transduction

الوصف: Saussurea lappa Dence (Compositae) is used as a traditional herbal medicine to treat abdominal pain and tenesmus in East Asia. Current studies have shown that S. lappa has anticancer activity in divergent of cancer cells. However, the effects of S. lappa on oral cancer and its mechanisms of action have yet to be elucidated.To explore its potential chemotherapeutic effects and mechanism of cell growth inhibition on human oral cancer cells.The dried roots of S. lappa were used in this study. Cell viability of KB cells was evaluated by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide assay after treatment with 30 µg/ml of methanol extract from the dried roots of S. lappa. To understand whether its effect on cell death is related with apoptosis pathway, we performed DNA fragmentation assay, western blot, caspase activity assay and fluorescence-activated cell sorting (FACS) analysis.Treatment of S. lappa extract onto KB cells reduced cell viability significantly with an IC50 value of 30 µg/ml. The formation of a DNA ladder was observed starting at the 24 h treatment. In western blotting analysis, the S. lappa extract induced the proteolytic processing of caspase-3, -9 and poly (ADP-ribose) polymerase, a significant increase of Bax and marked reduction of Bcl-2. We also confirmed the activation of caspase-3/-7 in living KB cells by fluorescence microscopy.These results suggested that S. lappa extract inhibited cell proliferation through the apoptosis pathway in KB human oral cancer cells.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc6060c179434c67cca750f4f991cd19Test
https://pubmed.ncbi.nlm.nih.gov/23855888Test

المؤلفون: Hong Sung Chun, Sung-Min Moon, Min-Gyeong Park, Do Kyung Kim, Myoung-Hwa Lee, Jin-Soo Kim, Chun Sung Kim, Su-Gwan Kim, Sun Young Park, Sun-Kyoung Yu, Sook-Young Lee, Jae-Sung Kim, Heung-Joong Kim, Ji-Su Oh, Mi-Ra Park, Seul Ah Lee

المصدر: Molecular and cellular biochemistry. 387(1-2)

مصطلحات موضوعية: Cell Survival, Clinical Biochemistry, Apoptosis, Biology, medicine.disease_cause, Axin Protein, Cell Line, Tumor, microRNA, medicine, Humans, Molecular Biology, 3' Untranslated Regions, Binding Sites, Oncogene, Base Sequence, Cell growth, Cell Biology, General Medicine, Transfection, Molecular biology, Gene Expression Regulation, Neoplastic, MicroRNAs, Cell culture, Cancer cell, Cancer research, Mouth Neoplasms, RNA Interference, Carcinogenesis

الوصف: MicroRNA (miRNA) is a small noncoding RNA molecule, 19–25 nucleotides in length, which regulates several pathways including cell development, cell proliferation, carcinogenesis, apoptosis, etc. In this study, the over-expression of microRNA-205 (miR-205) increased the number of apoptotic cells by at least 4 times compared to the control. In addition, over-expressed miRNA in KB oral cancer cells triggered apoptosis via the caspase cascade, including the cleavage of caspase-9, caspase-7, caspase-3, and PARP. Flow cytometry showed that apoptotic cell death was increased significantly by 35.33 % in KB oral cancer cells with over-expressed miR-205 compared to the control. The microarray data showed that axis inhibitor protein 2 (Axin2) was down-regulated in KB oral cancer cells transfected with miR-205. In addition, Axin2 was down-regulated by approximately 50 % by over-expressed miR-205 at both the mRNA and protein levels. Interestingly, Axin2 was up-regulated in KB oral cancer compared to human normal oral keratinocytes. Furthermore, the cell cytotoxicity and apoptotic population of KB oral cancer cells were increased significantly after Axin2 siRNA transfection. These results suggest that Axin2 is might be as potential oncogene in KB oral cancer cells. The luciferase assay showed that over-expressed miR-205 in KB oral cancer cells suppressed AXIN2 expression through an interaction with its own binding site at AXIN2 3′UTR (64–92). These results suggest that miR-205 is a novel anti-oncogenic miRNA in KB oral cancer cells, and may have potential applications in oral cancer therapy.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f6da769075343de030857a5ad7adc18dTest
https://pubmed.ncbi.nlm.nih.gov/24166197Test