التفاصيل البيبلوغرافية
| العنوان: |
Table_1_A Strong Decrease in TIMP3 Expression Mediated by the Presence of miR-17 and 20a Enables Extracellular Matrix Remodeling in the NSCLC Lesion Surroundings.DOCX |
| المؤلفون: |
Karolina H. Czarnecka, Bartosz Szmyd, Magda Barańska, Marcin Kaszkowiak, Jacek Kordiak, Adam Antczak, Dorota Pastuszak-Lewandoska, Ewa Brzeziańska-Lasota |
| سنة النشر: |
2019 |
| المجموعة: |
Frontiers: Figshare |
| مصطلحات موضوعية: |
Cancer, Cancer Cell Biology, Cancer Diagnosis, Cancer Genetics, Cancer Therapy (excl. Chemotherapy and Radiation Therapy), Chemotherapy, Haematological Tumours, Molecular Targets, Radiation Therapy, Solid Tumours, Oncology and Carcinogenesis not elsewhere classified, NSCLC molecular diagnostic markers, miRNA regulation, microRNA, extracellular matrix remodeling, metalloproteinases, tissue inhibitors of metalloproteinases, exosomes |
| الوصف: |
Background: Lung cancer is one of the most common causes of death worldwide with a relatively high fatality rate and a mean 5-years survival of about 18%. One of the hallmarks of cancer is the extracellular matrix (ECM) remodeling, which is crucial for metastasis. This process may be regulated by miRs targeting metalloproteinases (MMPs) associated with the ECM breakdown and metastatic process or blocking the action of tissue inhibitors of metalloproteinases (TIMPs). Search for early biomarkers is essential in detecting non-small cell lung cancer (NSCLC) and distinguishing its subtypes: Adenocarcinoma (AC) from Squamous Cell Carcinoma (SCC), enabling targeted chemotherapy. Methods:MiR-17 and miR-20a targeting MMP2 and TIMP3 were selected by TCGA data analysis with further validation using miRTarBase and literature. The study group comprised 47 patients with primary NSCLC (AC and SCC subtypes). RNA was isolated from the tumor and normal-looking neighboring tissue (NLNT) free of cancer cells. MiRs from peripheral blood exosomes were extracted on admission and 5–7 days after surgery. Gene and miRs expression were assessed in qPCR using TaqMan probes. Results: The MMP2 has been expressed on a similar level in NLNT, as in cancer. While, TIMP3 expression was decreased both in cancer tissue and NLNT, with significantly lower expression in cancer. TIMP3 downregulation in NLNT and in SCC subtype correlated negatively with miR-20a. The preoperative miR-17 expression was significantly higher among patients with SCC compared to AC. Receiver operating characteristic (ROC) analysis of miR-17 as AC subtype classifier revealed 90% specificity and 48% sensitivity in optimal cut-off point with area under ROC curve (AUC): 0.71 (95%CI: 0.55–0.87). Within NSCLC subtypes: a strong negative correlation between pack-years (PY) and TIMP3 expression was observed for NLNT in the SCC group. Conclusion: The TIMP3 silencing observed in the NLNT and its negative correlation with presurgical expression of miR-20a (from serum exosomes), suggest ... |
| نوع الوثيقة: |
dataset |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/dataset/Table_1_A_Strong_Decrease_in_TIMP3_Expression_Mediated_by_the_Presence_of_miR-17_and_20a_Enables_Extracellular_Matrix_Remodeling_in_the_NSCLC_Lesion_Surroundings_DOCX/11363444Test |
| DOI: |
10.3389/fonc.2019.01372.s001 |
| الإتاحة: |
https://doi.org/10.3389/fonc.2019.01372.s001Test
https://figshare.com/articles/dataset/Table_1_A_Strong_Decrease_in_TIMP3_Expression_Mediated_by_the_Presence_of_miR-17_and_20a_Enables_Extracellular_Matrix_Remodeling_in_the_NSCLC_Lesion_Surroundings_DOCX/11363444Test |
| حقوق: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.2FA49701 |
| قاعدة البيانات: |
BASE |
