Impact on NK cell functions of acute versus chronic exposure to extracellular vesicle-associated MICA. Dual role in cancer immunosurveillance
| العنوان: | Impact on NK cell functions of acute versus chronic exposure to extracellular vesicle-associated MICA. Dual role in cancer immunosurveillance |
|---|---|
| المؤلفون: | Elisabetta Vulpis, Luisa Loconte, Agnese Peri, Rosa Molfetta, Giulio Caracciolo, Laura Masuelli, Luana Tomaipitinca, Giovanna Peruzzi, Sara Petillo, Maria Teresa Petrucci, Francesca Fazio, Lucilla Simonelli, Cinzia Fionda, Alessandra Soriani, Cristina Cerboni, Marco Cippitelli, Rossella Paolini, Giovanni Bernardini, Gabriella Palmieri, Angela Santoni, Alessandra Zingoni |
| المصدر: | Journal of Extracellular Vesicles Journal of Extracellular Vesicles, Vol 11, Iss 1, Pp n/a-n/a (2022) |
| بيانات النشر: | Wyiey, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Male, Histology, chemical and pharmacologic phenomena, Ligands, Cell Line, NKG2D, Immunomodulation, Interferon-gamma, Bone Marrow, Humans, cancer, MICA, natural killer cells, extracellular vesicles, immune evasion, Extracellular Signal-Regulated MAP Kinases, Immunologic Surveillance, Research Articles, Aged, Aged, 80 and over, QH573-671, Cell Death, Histocompatibility Antigens Class I, hemic and immune systems, Cell Biology, Middle Aged, Killer Cells, Natural, NK Cell Lectin-Like Receptor Subfamily K, Female, Tumor Escape, Cytology, Multiple Myeloma, Research Article |
| الوصف: | Natural killer (NK) cells are innate cytotoxic lymphocytes that play a key role in cancer immunosurveillance thanks to their ability to recognize and kill cancer cells. NKG2D is an activating receptor that binds to MIC and ULBP molecules typically induced on damaged, transformed or infected cells. The release of NKG2D ligands (NKG2DLs) in the extracellular milieu through protease‐mediated cleavage or by extracellular vesicle (EV) secretion allows cancer cells to evade NKG2D‐mediated immunosurveillance. In this work, we investigated the immunomodulatory properties of the NKG2D ligand MICA*008 associated to distinct populations of EVs (i.e., small extracellular vesicles [sEVs] and medium size extracellular vesicles [mEVs]). By using as model a human MICA*008‐transfected multiple myeloma (MM) cell line, we found that this ligand is present on both vesicle populations. Interestingly, our findings reveal that NKG2D is specifically involved in the uptake of vesicles expressing its cognate ligand. We provide evidence that MICA*008‐expressing sEVs and mEVs are able on one hand to activate NK cells but, following prolonged stimulation induce a sustained NKG2D downmodulation leading to impaired NKG2D‐mediated functions. Moreover, our findings show that MICA*008 can be transferred by vesicles to NK cells causing fratricide. Focusing on MM as a clinically and biologically relevant model of tumour‐NK cell interactions, we found enrichment of EVs expressing MICA in the bone marrow of a cohort of patients. All together our results suggest that the accumulation of NKG2D ligands associated to vesicles in the tumour microenvironment could favour the suppression of NK cell activity either by NKG2D down‐modulation or by fratricide of NK cell dressed with EV‐derived NKG2D ligands. |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::50047175ed9921cdbe61773eda33c935Test http://hdl.handle.net/11573/1602470Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....50047175ed9921cdbe61773eda33c935 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |