Background . Yukon, a territory in northern Canada, has one of the highest reported sexually transmitted chlamydia infection rates in the country. Objective . We examined screening practices among physicians and community nurses to elucidate factors that may be contributing to the high rates. Design . Cross-sectional survey. Methods . A questionnaire was distributed to all physicians in Yukon and all community nurses in Yukon's communities. We surveyed sexual health assessment frequency, chlamydia testing frequency and barriers to screening. Comparison of physician testing practices was performed to another Canadian jurisdiction, which previously undertook a similar survey. Survey results were compared to the available laboratory data in Yukon. Results . Eligible physicians and nurses, 79% and 77%, respectively, participated in the survey. Physicians tested 15 to 24-year-old females more frequently than 15 to 24-year-old males for chlamydia (p=0.007). Physicians who asked sexual health assessment questions were more likely to test for chlamydia in both females (p