المؤلفون: Novisel Veitía Rodríguez, Lourdes R. García, Miriam Ramírez-López, Leonardo Rivero Quintana, Amanda Martirena Ramírez, Damaris Torres, Raúl Collado López

المصدر: Acta Biológica Colombiana. 24:13-25

مصطلحات موضوعية: food.ingredient, biology, Organogenesis, biology.organism_classification, Horticulture, food, Germination, Callus, Shoot, Cultivar, Phaseolus, General Agricultural and Biological Sciences, Cotyledon, Explant culture

الوصف: El mejoramiento genético convencional en frijol común resulta difícil debido a que presenta una base genética estrecha y muy estable. En este sentido, la combinación de la mutagénesis y el cultivo de tejidos, es una alternativa para inducir variabilidad genética en la búsqueda de tolerancia a factores bióticos y abióticos. Es por ello, que el presente trabajo tuvo como objetivo determinar el efecto de diferentes explantes irradiados en la regeneración in vitro de frijol común (Phaseolus vulgaris L.) cultivar “ICA Pijao”. Se aplicaron radiaciones gamma en callos, en el nudo cotiledonal con un cotiledón (NC-1) con dosis de 0, 10, 20, 30, 40, 50 y 60 Gy y semillas con 0, 100, 200, 300 y 400 Gy. Se evaluó el porcentaje de germinación, longitud de las raíces, porcentaje de explantes que formaron callos, masa fresca (g) de los callos, número de brotes por callo y el número de brotes con raíces. La radiación gamma provocó una disminución en la masa fresca del callo y NC-1. Los callos y el NC-1 solamente formaron brotes con las dosis de 10 y 20 Gy, pero estos fueron hiperhíricos. Los resultados demostraron que la semilla irradiada fue el explante con el que se logró la regeneración in vitro de plantas con hojas definidas, por lo que se recomienda como explante inicial para el uso combinado de mutagénesis y regeneración in vitro de plantas para el cultivar P. vulgaris “ICA Pijao” a través de la organogénesis indirecta.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::50090ac69eabf15f0050768b4d652c09Test
https://doi.org/10.15446/abc.v24n1.70422Test

المؤلفون: Lourdes R. García, Carlos Romero, Novisel Veitía, Damaris Torres, Geert Angenon, Idalmis Bermúdez-Caraballoso, Amanda Martirena-Ramírez, Raúl Collado

المساهمون: Department of Bio-engineering Sciences, Plant Genetics

مصطلحات موضوعية: biology, Agrobacterium, fungi, Organogenesis, Agrobacterium tumefaciens, Horticulture, biology.organism_classification, Molecular biology, Transformation (genetics), Plasmid, Callus, Botany, Phaseolus, Selectable marker

الوصف: A protocol for genetic transformation of Phaseolus vulgaris L. cv. CIAP7247F via Agrobacterium tumefaciens was established. Primary green nodular calli and proliferative calli were used as target explants. Several factors such as Agrobacterium strain, plasmid, light conditions, bacterial concentration, co-cultivation period and type of callus were studied for optimization of the Agrobacterium-mediated transformation. The highest DNA transfer occurred when proliferative calli were inoculated with strain EHA105 harbouring pCAMBIA3301 plasmid at a density of OD600 = 0.5, and co-cultivated under 16 h light/8 h dark photoperiod for 6 days. The transformation system integrates Agrobacterium-mediated DNA transfer with efficient regeneration via indirect organogenesis, and using the bar gene as selectable marker and glufosinate ammonium (herbicide finale) for callus selection. The proposed system allowed obtaining transgenic lines with Mendelian inheritance of the transgenes, as demonstrated by PCR analyses. These results also validated the effectiveness of a regeneration protocol via indirect organogenesis for regeneration of transformed bean cells. This system constitutes a major initial step for common bean transformation using A. tumefaciens.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cdb33de6b50e8608ca2e20940887149aTest
https://doi.org/10.1016/j.scienta.2015.06.046Test

المؤلفون: J.L. Rodríguez Lorenzo, Lourdes R. García, Carlos Romero, Novisel Veitía, Raúl Collado, Damaris Torres, Idalmis Bermúdez-Caraballoso, Geert Angenon

المساهمون: Plant Genetics, Department of Bio-engineering Sciences

مصطلحات موضوعية: common bean, food.ingredient, Callus formation, fungi, food and beverages, Organogenesis, Horticulture, Biology, Phaseolus vulgaris, cotyledonary node, food, indirect organogenesis, Germination, Callus, callus, Shoot, Botany, Regeneration, Cultivar, Cotyledon, Explant culture

الوصف: An efficient protocol for the in vitro regeneration of Phaseolus vulgaris L. cv. CIAP7247F via indirect organogenesis was established. Cotyledonary nodes, cotyledonary nodes with one cotyledon and cotyledonary nodes with two cotyledons dissected from the embryonic axis of three-day-old germinated seeds, were used as primary explants. Seeds of different age were used for callus induction. Different concentrations of thidiazuron (TDZ) and 6-benzylaminopurine (BAP) were assessed for callus proliferation and shoot regeneration. Five cultivars were tested to determine the effect of genotype. Cotyledonary nodes with one and two cotyledons from fresh and four-month-old seeds were the most effective explants for callus formation. Callus proliferation medium containing 0.04 mg l −1 of TDZ was optimum for proliferation of calli. A shoot regeneration frequency of approximately 3.0 shoots per callus was obtained on medium supplemented with 2.25 or 4.50 mg l −1 of BAP. Efficient rooting of plantlets was achieved on shoot elongation and rooting medium. Regenerated in vitro plants grown in the greenhouse showed normal development and were fertile. Although different responses were observed depending on genotype, the protocol was efficiently applied for different commercial P. vulgaris cultivars (BAT93, BAT304, BAT482 and ICA Pijao), demonstrating the value of this procedure.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26b03e10c88643d7d25a5895b2aceb90Test
https://biblio.vub.ac.be/vubir/efficient-in-vitro-plant-regeneration-via-indirect-organogenesis-for-different-common-bean-cultivarsTest(00e1e17b-dcd0-4724-8639-110e8a07be17).html