التفاصيل البيبلوغرافية
| العنوان: |
Standardization and validation of ATP luminometry as a diagnostic tool to assess the cleanliness of feeding equipment in preweaning calves. |
| المؤلفون: |
Chancy, Anaïs1 (AUTHOR), Santschi, Débora E.2 (AUTHOR), Paquet, Éric R.3 (AUTHOR), Renaud, David L.4 (AUTHOR), Gauthier, Marie-Lou5 (AUTHOR), Charbonneau, Édith3 (AUTHOR), Barbeau-Grégoire, Nicolas1 (AUTHOR), Van Driessche, Laura1 (AUTHOR), Buczinski, Sébastien1 (AUTHOR) s.buczinski@umontreal.ca |
| المصدر: |
Journal of Dairy Science. Sep2023, Vol. 106 Issue 9, p6263-6274. 12p. |
| مصطلحات موضوعية: |
*ANIMAL herds, *CALVES, *DAIRY farm management, *DAIRY cattle, *BACTERIAL cultures, *INTRACLASS correlation, *CATTLE feeding & feeds, *HYGIENE |
| مصطلحات جغرافية: |
QUEBEC (Quebec) |
| مستخلص: |
The objective of this cross-sectional study was to standardize a reliable and repeatable swabbing technique using ATP luminometry (light emission proportional to the amount of ATP with result provided in relative light units [RLU]) to describe the cleanliness of various feeding equipment used for preweaning calves in dairy farms. A total of 7 Québec commercial dairy herds were selected conveniently. Following visual hygiene scoring, the cleanliness of every available piece of feeding equipment was assessed using direct surface swabbing for buckets and nipples with Hygiena UltraSnap swabs. A liquid rinsing technique was used for esophageal feeders, bottles, and automatic milk feeders (AMF) with UltraSnap, AquaSnap, and MicroSnap swabs. To validate direct swabbing technique of buckets, a stage within and between operators was realized, as well as a conventional bacterial culture. A total of 519 swab samples were obtained from 201 pieces of equipment. The median (interquartile range) contamination in RLU for a bottle, esophageal feeder, AMF, bucket and nipple was 2 (1;6), 2 (0;12), 52 (19;269), 886 (128;7,230) and 899 (142;6,928), respectively. The direct swabbing technique, which consists in swabbing directly the surface of an equipment, showed excellent correlation for intrarater reliability (intraclass correlation (ICC) = 0.93; 95% CI: 0.88–0.96). The interoperator (2 sessions with 3 different operators) reliability also showed high correlation (ICC = 0.88; 95% CI: 0.78–0.94 for the first session, and ICC = 0.89; 95% CI: 0.79–0.95 for the second session). Luminometer values were positively associated with the visual score of esophageal feeders, AMF and buckets. A positive correlation between bacterial culture and direct swabbing of buckets was also found for the UltraSnap (r s = 0.653; 95% CI: 0.283–0.873; P = 0.0003) and MicroSnap (r s = 0.569, 95% CI: 0.309–0.765; P = 0.002). This study describes a standardized and practical on-farm swabbing technique for assessing the hygienic status of feeding equipment by luminometry, which can be integrated in the investigation of preweaning dairy calves problems. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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