دورية أكاديمية
Calcium Triggers Exocytosis from Two Types of Organelles in a Single Astrocyte
| العنوان: | Calcium Triggers Exocytosis from Two Types of Organelles in a Single Astrocyte |
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| المؤلفون: | Liu, Tao, Sun, Lei, Xiong, Yingfei, Shang, Shujiang, Guo, Ning, Teng, Sasa, Wang, Yeshi, Liu, Bin, Wang, Changhe, Wang, Li, Zheng, Lianghong, Zhang, Claire Xi, Han, Weiping, Zhou, Zhuan |
| المساهمون: | Zhang, CX (reprint author), Peking Univ, Inst Mol Med, State Key Lab Biomembrane & Membrane Biotechnol, 5 Yiheyuan Rd, Beijing 100871, Peoples R China., Peking Univ, Inst Mol Med, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100871, Peoples R China., Agcy Sci Technol & Res, Singapore Bioimaging Consortium, Lab Metab Med, Singapore 138667, Singapore., Peking Univ, Inst Mol Med, State Key Lab Biomembrane & Membrane Biotechnol, 5 Yiheyuan Rd, Beijing 100871, Peoples R China. |
| المصدر: | PubMed ; SCI |
| بيانات النشر: | journal of neuroscience |
| سنة النشر: | 2011 |
| المجموعة: | Peking University Institutional Repository (PKU IR) / 北京大学机构知识库 |
| مصطلحات موضوعية: | DEPENDENT GLUTAMATE RELEASE, LONG-TERM POTENTIATION, SYNAPTIC VESICLES, VESICULAR COMPARTMENT, REGULATED EXOCYTOSIS, LYSOSOMAL EXOCYTOSIS, CA2+ SENSOR, D-SERINE, RECEPTOR, FUSION |
| الوصف: | Astrocytes release a variety of signaling molecules including glutamate, D-serine, and ATP in a regulated manner. Although the functions of these molecules, from regulating synaptic transmission to controlling specific behavior, are well documented, the identity of their cellular compartment(s) is still unclear. Here we set out to study vesicular exocytosis and glutamate release in mouse hippocampal astrocytes. We found that small vesicles and lysosomes coexisted in the same freshly isolated or cultured astrocytes. Both small vesicles and lysosome fused with the plasma membrane in the same astrocytes in a Ca2+-regulated manner, although small vesicles were exocytosed more efficiently than lysosomes. Blockade of the vesicle glutamate transporter or cleavage of synaptobrevin 2 and cellubrevin (both are vesicle-associated membrane proteins) with a clostridial toxin greatly inhibited glutamate release from astrocytes, while lysosome exocytosis remained intact. Thus, both small vesicles and lysosomes contribute to Ca2+-dependent vesicular exocytosis, and small vesicles support glutamate release from astrocytes. ; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000292921100018&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Test ; Neurosciences ; SCI(E) ; PubMed ; 47 ; ARTICLE ; 29 ; 10593-10601 ; 31 |
| نوع الوثيقة: | journal/newspaper |
| اللغة: | English |
| تدمد: | 0270-6474 |
| العلاقة: | JOURNAL OF NEUROSCIENCE.2011,31,(29),10593-10601.; 662539; http://hdl.handle.net/20.500.11897/343642Test; WOS:000292921100018 |
| DOI: | 10.1523/JNEUROSCI.6401-10.2011 |
| الإتاحة: | https://doi.org/20.500.11897/343642Test https://doi.org/10.1523/JNEUROSCI.6401-10.2011Test https://hdl.handle.net/20.500.11897/343642Test |
| رقم الانضمام: | edsbas.98AEA1E7 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 02706474 |
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| DOI: | 10.1523/JNEUROSCI.6401-10.2011 |