المؤلفون: Guangwen Zhang, Xichun Peng, Liu Liu, Junsheng Liu

المصدر: International Journal of Biological Macromolecules. 189:346-355

مصطلحات موضوعية: Male, education, Prostatitis, Pharmacology, Gut flora, Coriobacteriaceae, digestive system, Biochemistry, Rats, Sprague-Dawley, chemistry.chemical_compound, Polysaccharides, Structural Biology, Prostate, Animals, Medicine, Molecular Biology, Phylogeny, health care economics and organizations, Testosterone, biology, business.industry, Finasteride, Organ Size, General Medicine, biology.organism_classification, medicine.disease, Gastrointestinal Microbiome, medicine.anatomical_structure, chemistry, Dihydrotestosterone, Chronic Disease, Androgens, Cytokines, Inflammation Mediators, business, Biomarkers, Wolfiporia, medicine.drug, Ruminococcaceae

الوصف: Finasteride is an antiandrogenic drug used for the clinical treatment of chronic nonbacterial prostatitis (CNP). Recently, we reported the anti-CNP activity of Poria cocos polysaccharides (PPs) in a rat model. In this study, we compared the differences between PPs and finasteride in treating CNP, especially their effects on the gut microbiota. Results showed that both PPs and finasteride significantly reduced the prostate weight and prostate index of CNP rats, and improved the histological damages in the inflamed prostate. Moreover, PPs and finasteride inhibited the production of pro-inflammatory cytokines (TNF-α, IL-2 and IL-8) and androgens (dihydrotestosterone and testosterone). By 16S rDNA sequencing, PPs and finasteride were found to reprogram the gut microbiota into distinct profiles. Further analysis presented that PPs but not finasteride recovered CNP-induced changes in the gut microbiota, including Ruminococcaceae NK4A214 group, uncultured bacterium f Ruminococcaceae, Ruminiclostridium 9, Phascolarctobacterium, Coriobacteriaceae UCG-002 and Oribacterium. LDA effect size (LEfSe) analysis revealed that PPs recovered the gut microbiota by targeting Ruminococcaceae NK4A214 group. Our results suggested that PPs alleviated CNP via different mechanisms from finasteride, especially by regulating the gut microbiota, which offers therapeutic target for the treatment of CNP.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a39b1c0094ad6fac15cc9b9db60f8606Test
https://doi.org/10.1016/j.ijbiomac.2021.08.139Test

المؤلفون: Jingnan Li, Chu-Yan Chen, Peng-Guang Yan, Ke-Min Li, Xu-Xia He, Xiangchen Meng, Ying-He Li

المصدر: World Journal of Gastroenterology

مصطلحات موضوعية: medicine.medical_specialty, Intermicrobial correlation, China, Intestinal microbiota, Observational Study, Disease, Gastroenterology, Feces, Internal medicine, RNA, Ribosomal, 16S, medicine, Humans, In patient, Intestinal Mucosa, Gastrointestinal tract, Chinese, Bacteria, business.industry, Lachnospiraceae, Significant difference, Fungi, General Medicine, medicine.disease, Ulcerative colitis, Gastrointestinal Microbiome, Dysbiosis, Colitis, Ulcerative, business, Ruminococcaceae

الوصف: BACKGROUND Dysbacteriosis may be a crucial environmental factor for ulcerative colitis (UC). Further study is required on microbiota alterations in the gastrointestinal tract of patients with UC for better clinical management and treatment. AIM To analyze the relationship between different clinical features and the intestinal microbiota, including bacteria and fungi, in Chinese patients with UC. METHODS Eligible inpatients were enrolled from January 1, 2018 to June 30, 2019, and stool and mucosa samples were collected. UC was diagnosed by endoscopy, pathology, Mayo Score, and Montreal classification. Gene amplicon sequencing of 16S rRNA gene and fungal internal transcribed spacer gene was used to detect the intestinal microbiota composition. Alpha diversity, principal component analysis, similarity analysis, and Metastats analysis were employed to evaluate differences among groups. RESULTS A total of 89 patients with UC and 33 non-inflammatory bowel disease (IBD) controls were enrolled. For bacterial analysis, 72 stool and 48 mucosa samples were obtained from patients with UC and 21 stool and 12 mucosa samples were obtained from the controls. For fungal analysis, stool samples were obtained from 43 patients with UC and 15 controls. A significant difference existed between the fecal and mucosal bacteria of patients with UC. The α-diversity of intestinal bacteria and the relative abundance of some families, such as Lachnospiraceae and Ruminococcaceae, decreased with the increasing severity of bowel inflammation, while Escherichia-Shigella showed the opposite trend. More intermicrobial correlations in UC in remission than in active patients were observed. The bacteria-fungi correlations became single and uneven in patients with UC. CONCLUSION The intestinal bacteria flora of patients with UC differs significantly in terms of various sample types and disease activities. The intermicrobial correlations change in patients with UC compared with non-IBD controls.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bf8f6e07e110d63d34d72b10806aa142Test
http://europepmc.org/articles/PMC8326252Test

المؤلفون: Kurosh Djafarian, Hadi Emamat, Hadith Tangestani, Hossein Khosravi Boroujeni, Sakineh Shab-Bidar

المصدر: Clinical Nutrition Research

مصطلحات موضوعية: History, food.ingredient, Population, Physiology, Review Article, Gut flora, digestive system, Coprococcus, vitamin D deficiency, Education, food, Vitamin D and neurology, Medicine, Microbiome, Vitamin D, education, education.field_of_study, biology, business.industry, Microbiota, Akkermansia, biology.organism_classification, medicine.disease, Computer Science Applications, Intestines, business, Ruminococcaceae

الوصف: Recently several studies have attempted to investigate the association between vitamin D and microbiota. However, studies have reported inconsistent results. This narrative review aimed to investigate the potential association between vitamin D and microbiota population in the gut by pooling together the results from observational studies and clinical trials. We considered animal and human studies in this field. Several studies have shown the correlation of vitamin D deficiency with microbiota. Furthermore, interventional studies were emerging that vitamin D change the microbiota composition in which leads to an increase in beneficial bacteria, such as Ruminococcaceae, Akkermansia, Faecalibacterium, and Coprococcus while decreases in Firmicutes. Vitamin D could change the microbiota toward decreasing in Firmicutes and increasing in Bacteroidetes. At genera level, vitamin D may connect to some genera of Lachnospiaceae family (e.g., Blautia, Rosburia, Dorea, and Coprococcus). It seems that adequate level of vitamin D is an important factor in improving the composition of the gut microbiota. More studies are needed to confirm possible underling mechanisms.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ea67bd05893593c2e7131eeefdcd6e9Test
http://europepmc.org/articles/PMC8331286Test

المؤلفون: Brian P. Carson, Cormac Powell, Alan E. Donnelly, Janas M. Harrington, Catherine M. Phillips, Patricia M. Kearney, Paul W. O'Toole, Xiaozhong Zhong, Seán R. Millar, Kieran P. Dowd, Ivan J. Perry

المساهمون: Athlone Institute of Technology, funded by a research grant from the Irish Health Research Board (reference: HRC/2007/2013) and the University of Limerick’s Department of Physical Education and Sport Sciences Postgraduate Scholarship Programme (2013 2017).

المصدر: The journal of nutrition, health & aging. 25:854-861

مصطلحات موضوعية: Male, Aging, 030309 nutrition & dietetics, Physical activity, Medicine (miscellaneous), Gut microbiota, Gut flora, 03 medical and health sciences, 0302 clinical medicine, RNA, Ribosomal, 16S, Humans, Medicine, Faculty of Science and Health AIT, 030212 general & internal medicine, Exercise, Aged, Bifidobacterium, Sedentary time, 0303 health sciences, Nutrition and Dietetics, biology, business.industry, Lachnospiraceae, Middle Aged, biology.organism_classification, Gut microbiome, Gastrointestinal Microbiome, Cross-Sectional Studies, Older adults, Cohort, Female, Sedentary Behavior, Geriatrics and Gerontology, business, Ireland, Ruminococcaceae, Demography

الوصف: Objectives: A 24-hour day is made up of time spent in a range of physical activity (PA) behaviours, including sleep, sedentary time, standing, light-intensity PA (LIPA) and moderate-to-vigorous PA (MVPA), all of which may have the potential to alter an individual’s health through various different pathways and mechanisms. This study aimed to explore the relationship between PA behaviours and the gut microbiome in older adults. Design: Cross-sectional study. Settings and Participants: Participants (n=100; age 67.76 years [3.02]; 44% female) from the Mitchelstown Cohort Rescreen (MCR) Study (2015-2017). Methods: Participants provided measures of gut microbiome composition (profiled by sequencing 16S rRNA gene amplicons), and objective measures of PA behaviours (by a 7-day wear protocol using an activPAL3 Micro). Results: Standing time was positively correlated with the abundance of butyrate-producing and anti-inflammatory bacteria, including Ruminococcaceae, Lachnospiraceae and Bifidobacterium, MVPA was positiv ely associated with the abundance of Lachnospiraceae bacteria, while sedentary time was associated with lower abundance of Ruminococcaceae and higher abundance of Streptococcus spp Conclusion: Physical activity behaviours appear to influence gut microbiota composition in older adults, with different PA behaviours having diverging effects on gut microbiota composition. yes

وصف الملف: PDF; application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2375223d0617b55e653f20ef129190d3Test
https://doi.org/10.1007/s12603-021-1630-6Test

المؤلفون: Suzan Wopereis, Guido J. E. J. Hooiveld, Mara P H van Trijp, Lydia A. Afman, Diederik Esser, Sophie Schutte, Femke P M Hoevenaars

المصدر: The Journal of Nutrition
Hepatobiliary Surg Nutr
The Journal of Nutrition, 151(3), 491-502
The Journal of Nutrition 151 (2020) 3

مصطلحات موضوعية: Dietary Fiber, Male, 0301 basic medicine, Flour, Medicine (miscellaneous), Overweight, Gut flora, Voeding, Metabolisme en Genomica, Feces, 0302 clinical medicine, Nonalcoholic fatty liver disease, fermentation, Whole Grains, Nutrition and Dietetics, biology, Fatty liver, Middle Aged, Metabolism and Genomics, Health & Consumer Research, Liver, Metabolisme en Genomica, Nutrition, Metabolism and Genomics, Female, 030211 gastroenterology & hepatology, medicine.symptom, Metabolic Networks and Pathways, Ruminococcaceae, medicine.medical_specialty, refined wheat, Aspartate transaminase, AcademicSubjects/MED00060, 03 medical and health sciences, Voeding, Double-Blind Method, Internal medicine, medicine, Humans, human, Food, Health & Consumer Research, VLAG, Nutrition, Aged, fatty liver, gut microbiota, business.industry, Biochemical, Molecular, and Genetic Mechanisms, overweight/obesity, medicine.disease, biology.organism_classification, Obesity, Gastrointestinal Microbiome, Gastrointestinal Tract, 030104 developmental biology, Endocrinology, Alanine transaminase, Food, Commentary, biology.protein, AcademicSubjects/SCI00960, whole grain wheat, Edible Grain, business, Biomarkers

الوصف: Background Whole grain wheat (WGW) products are advocated as a healthy choice when compared with refined wheat (RW). One proposed mechanism for these health benefits is via the microbiota, because WGW contains multiple fibers. WGW consumption has been proposed to ameliorate nonalcoholic fatty liver disease, in which microbiota might play a role. Objectives We investigated the effect of WGW compared with RW intervention on the fecal microbiota composition and functionality, and correlated intervention-induced changes in bacteria with changes in liver health parameters in adults with overweight or obesity. Methods We used data of a 12-wk double-blind, randomized, controlled, parallel trial to examine the effects of a WGW (98 g/d) or RW (98 g/d) intervention on the secondary outcomes fecal microbiota composition, predicted microbiota functionality, and stool consistency in 37 women and men (aged 45–70 y, BMI 25–35 kg/m2). The changes in microbiota composition, measured using 16S ribosomal RNA gene sequencing, after a 12-wk intervention were analyzed with nonparametric tests, and correlated with changes in liver fat and circulating concentrations of liver enzymes including alanine transaminase, aspartate transaminase, γ-glutamyltransferase, and serum amyloid A. Results The WGW intervention increased the mean (± SD) relative abundances of Ruminococcaceae_UCG-014 (baseline: 2.2 ± 4.6%, differential change over time (Δ) 0.51 ± 4.2%), Ruminiclostridium_9 (baseline: 0.065 ± 0.11%, Δ 0.054 ± 0.14%), and Ruminococcaceae_NK4A214_group (baseline: 0.37 ± 0.56%, Δ 0.17 ± 0.83%), and also the predicted pathway acetyl-CoA fermentation to butyrate II (baseline: 0.23 ± 0.062%, Δ 0.035 ± 0.059%), compared with the RW intervention (P values

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::38d7e93d9e4e2bef68a6ff4a87002fc2Test
https://doi.org/10.1093/jn/nxaa312Test

المؤلفون: Yeong-Jae Seok, Dong Ho Lee, Soo In Choi, Yeon Ran Kim, Ryoung Hee Nam, Huitae Min, Yong Sung Kim, Joo Hee Son, Kichul Yoon, Chin Hee Song, Jeong Eun Yu, Ji Hyun Park, Nayoung Kim

المصدر: Journal of Neurogastroenterology and Motility

مصطلحات موضوعية: medicine.medical_specialty, Aging, Butyrate, Gut flora, digestive system, 03 medical and health sciences, Cecum, 0302 clinical medicine, Internal medicine, medicine, chemistry.chemical_classification, biology, business.industry, Microbiota, Lachnospiraceae, Short-chain fatty acid, Gastroenterology, Fatty acid, biology.organism_classification, Rats, Endocrinology, Real-time polymerase chain reaction, medicine.anatomical_structure, chemistry, 030220 oncology & carcinogenesis, 030211 gastroenterology & hepatology, Original Article, Neurology (clinical), business, Ruminococcaceae

الوصف: Background/Aims The gut microbiota regulates intestinal immune homeostasis through host-microbiota interactions. Multiple factors affect the gut microbiota, including age, sex, diet, and use of drugs. In addition, information on gut microbiota differs depending on the samples. The aim of this study is to investigate whether changes in cecal microbiota depend on aging. Methods Gut microbiota in cecal contents of 6-, 31-, and 74-week-old and 2-year-old male Fischer-344 rats (corresponding to 5-, 30-, 60-, and 80-year-old humans in terms of age) were analyzed using 16S ribosomal RNA metagenome sequencing and phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) based on the Kyoto Encyclopedia of Genes and Genomes orthology. Moreover, short-chain fatty acid (SCFA) level in cecum and inflammation related factors were measured using real-time quantitative polymerase chain reaction and enzyme linked immunosorbent assay. Results Alpha and beta diversity did not change significantly with age. At the family level, Lachnospiraceae and Ruminococcaceae, which produce SCFAs, showed significant change in 31-week-old rats: Lachnospiraceae significantly increased at 31 weeks of age, compared to other age groups, while Ruminococcaceae decreased. Butyrate levels in cecum were significantly increased in 31-week-old rats, and the expression of inflammation related genes was increased followed aging. Especially, EU622775_s and EU622773_s, which were highly abundance species in 31-week-old rats, showed significant relationship with butyrate concentration. Enzymes required for producing butyrate―acetyl-CoA transferase, butyryl-CoA dehydrogenase, and butyrate kinase―were not predicted by PICRUSt. Conclusions Major bacterial taxa in the cecal lumen, such as Lachnospiraceae, well-known SCFAs-producing family, changed in 31-week-old rats. Moreover, unknown species EU622775_s and EU622773_s showed strong association with cecal butyrate level at 31 weeks of age. (J Neurogastroenterol Motil 2021;27:134-146)

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ddbd343b728b1351326d7619cc29f7cfTest
http://europepmc.org/articles/PMC7786083Test

المؤلفون: Mengjia Hu, Ying Zhang, Shujie Chen, Tao Yang, Lina Fan, Renbin Lin, Yawen Zhang, Misi Si, Luyi Chen, Jiamin He, Yadong Qi, Lan Wang

المصدر: Journal of Microbiology. 58:926-937

مصطلحات موضوعية: Adult, Male, Alcohol Drinking, Firmicutes, Physiology, Alcohol, Gut flora, Applied Microbiology and Biotechnology, Microbiology, Group A, Cigarette Smoking, Feces, Young Adult, 03 medical and health sciences, chemistry.chemical_compound, medicine, Humans, Aged, 030304 developmental biology, 0303 health sciences, Bacteria, biology, 030306 microbiology, business.industry, General Medicine, Middle Aged, Fatty Acids, Volatile, biology.organism_classification, medicine.disease, Healthy Volunteers, Gastrointestinal Microbiome, chemistry, Bacteroides, business, Dysbiosis, Ruminococcaceae

الوصف: Human intestinal microbiota is affected by the exogenous microenvironment. This study aimed to determine the effects of cigarettes and alcohol on the gut microbiota of healthy men. In total, 116 healthy male subjects were enrolled and divided into four groups: non-smoking and non-drinking (Group A), smoking only (Group B), drinking only (Group C), and smoking and drinking combined (Group D). Fecal samples were collected and sequenced using 16S rRNA to analyze the microbial composition. Short-chain fatty acid (SCFAs) levels in feces were determined by gas chromatography. We found that cigarette and alcohol consumptions can alter overall composition of gut microbiota in healthy men. The relative abundances of phylum Bacteroidetes and Firmicutes and more than 40 genera were changed with cigarette and alcohol consumptions. SCFAs decreased with smoking and alcohol consumption. Multivariate analysis indicated that when compared with group A, group B/C/D had higher Bacteroides, and lower Phascolarctobacterium, Ruminococcaceae_UCG-002, Ruminococcaceae_UCG-003, and Ruminiclostridium_9 regardless of BMI and age. Additionally, the abundance of Bacteroides was positively correlated with the smoking pack-year (r = 0.207, p < 0.05), the abundance of predicted pathway of bacterial toxins (r = 0.3672, p < 0.001) and the level of carcinoembryonic antigen in host (r = 0.318, p < 0.01). Group D shared similar microbial construction with group B, but exerted differences far from group C with lower abundance of Haemophilus. These results demonstrated that cigarette and alcohol consumption separately affected the intestinal microbiota and function in healthy men; furthermore, the co-occurrence of cigarette and alcohol didn't exacerbate the dysbiosis and cigarette played the predominated role on the alteration.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b003dd53cc743411c81cd2fc49517dcfTest
https://doi.org/10.1007/s12275-020-0006-7Test

المؤلفون: Yongsen Jia, Dingjie Xu, Yanyan Yu, Ying Cao, Chunhua Jiang

المصدر: Evidence-Based Complementary and Alternative Medicine, Vol 2020 (2020)
Evidence-based Complementary and Alternative Medicine : eCAM

مصطلحات موضوعية: Article Subject, Firmicutes, Endometriosis, Physiology, Gut flora, Endometrium, digestive system, Lesion, Other systems of medicine, 03 medical and health sciences, 0302 clinical medicine, medicine, 030304 developmental biology, 0303 health sciences, biology, business.industry, Letrozole, Bacteroidetes, medicine.disease, biology.organism_classification, medicine.anatomical_structure, Complementary and alternative medicine, 030220 oncology & carcinogenesis, medicine.symptom, business, RZ201-999, Research Article, Ruminococcaceae, medicine.drug

الوصف: We previously showed that the Chinese herbal medicine, Shaofu Zhuyu decoction (SFZYD), shrank the size of endometriotic lesions in rats with endometriosis. We therefore conducted the present study to investigate the effects of letrozole and SFZYD on gut microbiota in endometriotic rats. Rats were divided into four groups: a blank group, model group, letrozole group, and SFZY group. Ectopic lesion size and COX-2 expression in the endometrium and endometriotic lesions were compared, and the community of gut microbiota was detected using 16S rRNA gene sequencing. Both letrozole and SFZYD reduced the size of ectopic lesions as well as lowered the expression of COX-2, thus reducing the inflammatory response. Compared with the blank group, the α-diversity of gut microbiota in endometriotic rats decreased, the Firmicutes/Bacteroidetes ratio increased, and the abundance of Ruminococcaceae was reduced. The α-diversity of gut microbiota in the letrozole group was similar to that in the model group, but the Firmicutes/Bacteroidetes ratio was diminished. The α-diversity in the SFZY group was similar to that in the blank group, the Firmicutes/Bacteroidetes ratio was attenuated, and the abundance of Ruminococcaceae was elevated compared with the model group. These results indicated that the therapeutic mechanisms of both letrozole and SFZYD were related to the restoration of gut microbiota.

وصف الملف: text/xhtml

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e60d6ad747ef8008f3655d054c16d91Test
https://doi.org/10.1155/2020/3687498Test

المؤلفون: Qiong Shen, Mengdan Zhong, Ying Wu, Lei Chen, Beibei Gao, Mengdie Cao, Songwen Ju

المصدر: BMC Pregnancy and Childbirth, Vol 20, Iss 1, Pp 1-11 (2020)
BMC Pregnancy and Childbirth

مصطلحات موضوعية: Adult, Blood Glucose, Firmicutes, Physiology, 030209 endocrinology & metabolism, Gut microbiota, Gut flora, lcsh:Gynecology and obstetrics, Feces, 03 medical and health sciences, 0302 clinical medicine, Pregnancy, RNA, Ribosomal, 16S, Diabetes mellitus, 16S rDNA, Next generation sequencing, medicine, Humans, Bacteroidaceae, lcsh:RG1-991, 030304 developmental biology, 0303 health sciences, biology, business.industry, Clostridiales, Obstetrics and Gynecology, Bacteroidetes, biology.organism_classification, medicine.disease, Gastrointestinal Microbiome, Case-Control Studies, Hyperglycemia, Dysbiosis, Hyperglycaemia in early pregnancy, Female, business, Research Article, Ruminococcaceae

الوصف: BackgroundRecent studies suggest that there is a link between the gut microbiota and glucose metabolism. This study aimed to compare the gut microbiota during early pregnancy of women with hyperglycymia to those with normal blood glucose.MethodsGut microbial composition was analysed in 22 women with hyperglycaemia and 28 age-matched healthy controls during their first prenatal visits (ResultsWomen with hyperglycemia in pregnancy (HIP) had significantly lower microbial richness and diversity compared with healthy pregnant women. The proportions of theFirmicutesandBacteroidetesphyla and the ratio ofFirmicutes:Bacteroideteswere not different between the two groups. We observed that individuals with HIP had an increased abundance ofNocardiaceae,Fusobacteriaceae, etc., whereas healthy controls had significantly higher levels ofChristensenellaceae,Clostridiales_vadinBB60_group,Coriobacteriaceae, etc. Similarly, levels of the members of theRuminococcaceaefamily,includingRuminococcaceae_UCG-014,Ruminococcaceae_UCG-003, andRuminococcaceae_UCG-002,were significantly reduced in the HIP group and were negatively correlated with HbA1c. HbA1c levels were positively correlated withBacteroidaceaeandEnterobacteriaceaeand negatively correlated withChristensenellaceae, etc. CRP was positively correlated with theBacteroidaceaeandFusobacteriaceaefamilies and theFusobacteriumgenus.ConclusionsOur study revealed that individuals with HIP have gut microbial dysbiosis and that certain bacterial groups are associated with glucose metabolism during pregnancy. Further study is needed to provide new ideas to control glucose by modifying the gut microbiota.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::319ca975c5b384f7c2fd4910367b19b6Test
http://link.springer.com/article/10.1186/s12884-020-02961-5Test

المؤلفون: Christian Schulz, Peter Malfertheiner, Lukas Macke, Leandra Koletzko

المصدر: Alimentary Pharmacology & Therapeutics. 51:505-526

مصطلحات موضوعية: DNA, Bacterial, Gut flora, Bioinformatics, Cohort Studies, Feces, 03 medical and health sciences, 0302 clinical medicine, Humans, Medicine, Pharmacology (medical), 030212 general & internal medicine, Microbiome, Bacteria, Hepatology, biology, business.industry, Lachnospiraceae, Gastroenterology, High-Throughput Nucleotide Sequencing, Proton Pump Inhibitors, Sequence Analysis, DNA, biology.organism_classification, Streptococcaceae, medicine.disease, Gastrointestinal Microbiome, Gastrointestinal Tract, Bifidobacteriaceae, Observational Studies as Topic, Systematic review, Dysbiosis, 030211 gastroenterology & hepatology, business, Ruminococcaceae

الوصف: Background Proton pump inhibitors (PPI) are widely used to treat acid-related disorders of the upper gastrointestinal tract. However, large observational studies have raised concerns about PPI-associated adverse events. In recent years, data from next-generation sequencing studies suggested that PPIs affect the composition of the intestinal microbiota, while a balanced gut microbiome is essential for maintaining health. Aim To review the available evidence from next-generation sequencing studies on the effect of PPIs on the intestinal microbiome and to discuss possible implications of PPI-induced dysbiosis in health and disease. Methods A systematic review was conducted following the recommendations of the Preferred Reporting Items for Systematic reviews and Meta-Analyses statement. A PubMed query yielded 197 results. 19 publications met the prespecified eligibility criteria. Results Twelve observational study cohorts with 708 PPI users and 11 interventional cohorts with 180 PPI users were included in the review. In most studies, PPI treatment did not affect microbiological richness and diversity, but was associated with distinct taxonomic alterations: In the upper gastrointestinal tract, PPI users showed overgrowth of orally derived bacteria, mostly Streptococcaceae (findings based on six independent cohorts with 126 PPI users). In faecal samples, PPIs increased multiple taxa from the orders Bacillales (eg, Staphylococcaceae), Lactobacillales (eg, Enterococcaceae, Lactobacillaceae, Streptococcaceae) and Actinomycetales (eg, Actinomycetaceae, Micrococcaceae), the families Pasteurellaceae and Enterobacteriaceae and the genus Veillonella. Taxa decreased by PPIs include Bifidobacteriaceae, Ruminococcaceae, Lachnospiraceae and Mollicutes (findings in faecal samples based on 19 independent cohorts with 790 PPI users). Conclusion PPI use is associated with moderate alterations to upper and distal gut microbiota. The available data suggest that PPI-induced hypochlorhydria facilitates colonization of more distal parts of the digestive tract by upper gastrointestinal microbiota.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::418e4f0683f51b3c9b515ed20087b082Test
https://doi.org/10.1111/apt.15604Test