Inhibition of miR-328–3p Impairs Cancer Stem Cell Function and Prevents Metastasis in Ovarian Cancer
| العنوان: | Inhibition of miR-328–3p Impairs Cancer Stem Cell Function and Prevents Metastasis in Ovarian Cancer |
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| المؤلفون: | Xiaoli Zhang, Dayong Wu, David E. Cohn, Ri Cui, Lu Liu, Guozhen Xie, Ananya Banerjee, Zaibo Li, Karuppaiyah Selvendiran, Tiantian Cui, Amit Kumar Srivastava, Shurui Cai, Jinsong Liu, Srinivas Patnaik, Adam R. Karpf, Chunhua Han, Qi-En Wang |
| المصدر: | Cancer Research. 79:2314-2326 |
| بيانات النشر: | American Association for Cancer Research (AACR), 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Cancer Research, Population, Apoptosis, Mice, SCID, Article, Metastasis, Mice, 03 medical and health sciences, 0302 clinical medicine, Downregulation and upregulation, Cell Movement, Mice, Inbred NOD, Cancer stem cell, microRNA, Biomarkers, Tumor, Tumor Cells, Cultured, medicine, Animals, Humans, Neoplasm Metastasis, Extracellular Signal-Regulated MAP Kinases, education, Cell Proliferation, Ovarian Neoplasms, education.field_of_study, Cell growth, business.industry, Prognosis, medicine.disease, Xenograft Model Antitumor Assays, Gene Expression Regulation, Neoplastic, Survival Rate, MicroRNAs, 030104 developmental biology, Oncology, 030220 oncology & carcinogenesis, Neoplastic Stem Cells, Cancer research, Female, Ovarian cancer, business |
| الوصف: | Cancer stem cells (CSC) play a central role in cancer metastasis and development of drug resistance. miRNA are important in regulating CSC properties and are considered potential therapeutic targets. Here we report that miR-328–3p (miR-328) is significantly upregulated in ovarian CSC. High expression of miR-328 maintained CSC properties by directly targeting DNA damage binding protein 2, which has been shown previously to inhibit ovarian CSC. Reduced activity of ERK signaling in ovarian CSC, mainly due to a low level of reactive oxygen species, contributed to the enhanced expression of miR-328 and maintenance of CSC. Inhibition of miR-328 in mouse orthotopic ovarian xenografts impeded tumor growth and prevented tumor metastasis. In summary, our findings provide a novel mechanism underlying maintenance of the CSC population in ovarian cancer and suggest that targeted inhibition of miR-328 could be exploited for the eradication of CSC and aversion of tumor metastasis in ovarian cancer. Significance: These findings present inhibition of miR-328 as a novel strategy for efficient elimination of CSC to prevent tumor metastasis and recurrence in patients with epithelial ovarian cancer. |
| تدمد: | 1538-7445 0008-5472 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::780f100ef60fbf6293c2fff68badfcfdTest https://doi.org/10.1158/0008-5472.can-18-3668Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....780f100ef60fbf6293c2fff68badfcfd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15387445 00085472 |
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