المؤلفون: Jian-qun Su, Chen Wang, Suxia Wang, Li Yang, Xiaojuan Yu, Tao Su, Gang Liu, Pingping Sun, Jing Nie, Xu-jie Zhou

المصدر: Clinical immunology (Orlando, Fla.). 205

مصطلحات موضوعية: 0301 basic medicine, Adult, Male, Pathology, medicine.medical_specialty, Glomerulonephritis, Membranoproliferative, Urinary system, Immunology, Macrophage polarization, Cell Count, Urine, urologic and male genital diseases, Kidney, 03 medical and health sciences, Young Adult, 0302 clinical medicine, Glomerulonephritis, Glomerulopathy, medicine, Immunology and Allergy, Humans, Acute tubulointerstitial nephritis, medicine.diagnostic_test, urogenital system, business.industry, Thrombotic Microangiopathies, Macrophages, Acute kidney injury, Acute Kidney Injury, Kidney Tubular Necrosis, Acute, Middle Aged, medicine.disease, female genital diseases and pregnancy complications, 030104 developmental biology, medicine.anatomical_structure, Nephritis, Interstitial, Female, Renal biopsy, business, 030215 immunology

الوصف: Macrophage polarization is a major contributing factor in acute kidney injury (AKI). We aim to determine its biomarker value in differentiating etiologic causes of various intrinsic renal AKI. A total of 205 patients with renal intrinsic AKI were enrolled. Urinary sCD163 was quantified and macrophage subtypes in urine and in renal biopsy were determined. Compared to healthy controls and AKI due to interstitial or tubular injuries (0 pg/μmol), urinary sCD163 was markedly higher in glomerulopathy, especially in diffuse proliferative glomerulonephritis (275.5 pg/μmol) and significantly correlated with cellular crescent formation. Urine sediment analysis of M1/M2 ratio could differentiate acute tubulointerstitial nephritis (M1/M2 2.35) from crescentic glomerulonephritis (M1/M2 0.27). Urinary sCD163 levels and M2 subtype positively correlated with infiltrated M2 in the glomeruli, whereas urine M1 positively correlated with infiltrated M1 in the interstitium. Of note, urinary sCD163 showed better diagnositic performance in differentiating disease etiologies compared to tradiational urinary biomarkers of AKI (NGAL and KIM-1) and markers of myeloid cells (CD11b) and pan macrophages (CD68). Thus markers of macrophage polarization could be viewed as the noninvasive "liquid biopsy" in the presence of various intrinsic kidney diseases.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::692aab488ec9ce202bbda64dcfe192dbTest
https://pubmed.ncbi.nlm.nih.gov/31212026Test

المؤلفون: Xia Liu, Zhanguo Li, Hong Zhang, Xu Liu, Ru Li, Ping Zhu, Jing He, Xinyu Wu, Yi Zhao, Baoli Zhu, Xu-jie Zhou, Xiaolan Lu, Xiangyuan Liu, Jianhua Xu, Jianping Guo

المصدر: Annals of the Rheumatic Diseases. 70:1648-1651

مصطلحات موضوعية: Adult, Male, China, Linkage disequilibrium, Immunology, Single-nucleotide polymorphism, Polymerase Chain Reaction, Polymorphism, Single Nucleotide, Linkage Disequilibrium, General Biochemistry, Genetics and Molecular Biology, Arthritis, Rheumatoid, Young Adult, Chinese han population, Asian People, Gene Frequency, Rheumatology, Cornified Envelope Proline-Rich Proteins, Psoriasis, medicine, Humans, Lupus Erythematosus, Systemic, Immunology and Allergy, Genetic Predisposition to Disease, In patient, Allele, Aged, business.industry, Middle Aged, medicine.disease, Connective tissue disease, Case-Control Studies, Rheumatoid arthritis, Female, business, Gene Deletion

الوصف: Objectives The deletion of LCE3C_LCE3B confers susceptibility to psoriasis and rheumatoid arthritis (RA) in Caucasians. The aim of this study was to investigate the variant involvement in RA in the Chinese Han population and to further explore its potential role in the susceptibility to systemic lupus erythematosus (SLE). Methods LCE3C_LCE3B - del was genotyped in 898 patients with RA and 681 healthy controls. Two single nucleotide polymorphisms (SNPs, rs4112788 and rs4085613) in strong linkage disequilibrium with LCE3C_LCE3B - del were then genotyped in patients with RA (n=1222), SLE (n=870) and healthy controls (n=1031). Results The deletion of LCE3C_LCE3B and SNPs rs4112788 and rs4085613 showed an association with RA (allele analysis: p=7.72×10 −4 , OR 1.28, 95% CI 1.11 to 1.47; p=6.39×10 −4 , OR 1.23, 95% CI 1.09 to 1.38; and p=5.38×10 −4 , OR 1.23, 95% CI 1.10 to 1.39, respectively). The two SNPs were also significantly associated with SLE (allele analysis: p=7.68×10 −3 , OR 1.19, 95% CI 1.05 to 1.36 and p=5.30×10 −3 , OR 1.20, 95% CI 1.06 to 1.37). Conclusions This study provides evidence for an association between LCE3C_LCE3B - del and RA in non-Caucasian populations, and SNPs rs4112788 and rs4085613 tagging LCE3C_LCE3B - del were novel susceptibility factors for SLE.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ef567001a0c7933e204a54c4c732036fTest
https://doi.org/10.1136/ard.2010.148072Test