MiR-18a and miR-18b are expressed in the stroma of oestrogen receptor alpha negative breast cancers
| العنوان: | MiR-18a and miR-18b are expressed in the stroma of oestrogen receptor alpha negative breast cancers |
|---|---|
| المؤلفون: | Deirdre Cronin-Fenton, Ivar Skaland, Miriam Ragle Aure, Kristine Kleivi Sahlberg, Kristin Jonsdottir, Nina Egeland, Einar Gudlaugsson, Emiel A. M. Janssen, Jan P. A. Baak, Vessela N. Kristensen |
| المصدر: | BMC Cancer BMC Cancer, Vol 20, Iss 1, Pp 1-14 (2020) Egeland, N G, Jonsdottir, K, Aure, M R, Sahlberg, K, Kristensen, V N, Cronin-Fenton, D, Skaland, I, Gudlaugsson, E, Baak, J P A & Janssen, E A M 2020, ' MiR-18a and miR-18b are expressed in the stroma of oestrogen receptor alpha negative breast cancers ', BMC Cancer, vol. 20, no. 1, pp. 377 . https://doi.org/10.1186/s12885-020-06857-7Test 377 (2020) Egeland, N G, Jonsdottir, K, Aure, M R, Sahlberg, K, Kristensen, V N, Cronin-Fenton, D, Skaland, I, Gudlaugsson, E, Baak, J P A & Janssen, E A M 2020, ' MiR-18a and miR-18b are expressed in the stroma of oestrogen receptor alpha negative breast cancers ', BMC Cancer, vol. 20, no. 1, 377 . https://doi.org/10.1186/s12885-020-06857-7Test |
| بيانات النشر: | BioMed Central, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Cancer Research, Macrophages/immunology, Cohort Studies, 0302 clinical medicine, Breast cancer, Databases, Genetic, microRNA, CD68, Medisinske Fag: 700::Klinisk medisinske fag: 750::Onkologi: 762 [VDP], Middle Aged, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Gene Expression Regulation, Neoplastic, Oncology, 030220 oncology & carcinogenesis, Immunohistochemistry, Female, Biomarkers, Tumor/genetics, In situ hybridization, Research Article, Chromogenic in situ hybridization, Breast Neoplasms, Biology, Breast Neoplasms/genetics, lcsh:RC254-282, 03 medical and health sciences, Cytokeratin, Lymphocytes, Tumor-Infiltrating, Stroma, brystkreft, Estrogen Receptor alpha/metabolism, Genetics, medicine, Biomarkers, Tumor, Humans, CISH, Lymphocytes, Tumor-Infiltrating/immunology, Tumour microenvironment, Tumour associated macrophages (TAM), Macrophages, Estrogen Receptor alpha, medicine.disease, Databases, Genetic/statistics & numerical data, MicroRNAs/genetics, MicroRNAs, 030104 developmental biology, Stromal Cells/immunology, Cancer research, Stromal Cells |
| الوصف: | Background Previously, we have shown that miR-18a and miR-18b gene expression strongly correlates with high proliferation, oestrogen receptor -negativity (ER−), cytokeratin 5/6 positivity and basal-like features of breast cancer. Methods We investigated the expression and localization of miR-18a and -18b in formalin fixed paraffin embedded (FFPE) tissue from lymph node negative breast cancers (n = 40), by chromogenic in situ hybridization (CISH). The expression level and in situ localization of miR-18a and -18b was assessed with respect to the presence of tumour infiltrating lymphocytes (TILs) and immunohistochemical markers for ER, CD4, CD8, CD20, CD68, CD138, PAX5 and actin. Furthermore, in two independent breast cancer cohorts (94 and 377 patients) the correlation between miR-18a and -18b expression and the relative quantification of 22 immune cell types obtained from the CIBERSORT tool was assessed. Results CISH demonstrated distinct and specific cytoplasmic staining for both miR-18a and miR-18b, particularly in the intratumoural stroma and the stroma surrounding the tumour margin. Staining by immunohistochemistry revealed some degree of overlap of miR-18a and -18b with CD68 (monocytes/macrophages), CD138 (plasma cells) and the presence of high percentages of TILs. CIBERSORT analysis showed a strong correlation between M1-macrophages and CD4+ memory activated T-cells with mir-18a and -18b. Conclusions Our study demonstrates that miR-18a and miR-18b expression is associated with ER- breast tumours that display a high degree of inflammation. This expression is potentially associated specifically with macrophages. These results suggest that miR-18a and miR-18b may play a role in the systemic immunological response in ER− tumours. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1471-2407 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6b0c414d25157537f7229e3d0aae913cTest http://europepmc.org/articles/PMC7201801Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6b0c414d25157537f7229e3d0aae913c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14712407 |
|---|