المؤلفون: Lu, Shaolei¹ (AUTHOR) slu@lifespan.org, Yakirevich, Evgeny¹ (AUTHOR), Wang, Li Juan¹ (AUTHOR), Resnick, Murray B.¹ (AUTHOR), Wang, Yihong¹ (AUTHOR) ywang6@lifespan.org

المصدر: BMC Cancer. 11/12/2019, Vol. 19 Issue 1, p1-10. 10p. 1 Diagram, 5 Charts, 1 Graph.

مصطلحات موضوعية: *GATA proteins, *CARRIER proteins, *BREAST cancer, *KERATIN, *TRIPLE-negative breast cancer, *ESTROGEN receptors, *PROTEIN metabolism, *PROTEINS, *IMMUNOHISTOCHEMISTRY, *PROGNOSIS, *TUMOR classification, *RESEARCH funding, *BREAST tumors, *PROPORTIONAL hazards models, *TUMOR grading

مستخلص: Background: Cytokeratin 7 (CK7) and GATA binding protein 3 (GATA3) are considered as immunohistochemical hallmarks of breast cancers; however, there are breast tumors lacking these markers. Clinicopathological characterization of CK7 negative breast cancer has not been addressed previously and similar studies on GATA3 negative tumors are limited.Methods: This study included 196 consecutive cases of Nottingham Grade 3 breast cancers with 159 cases of Grade 1 and Grade 2 tumors for comparison. CK7 and GATA3 expression was correlated with patient's age, histological type, pathological grade and stage, hormone receptor status, molecular subtype and overall survival.Results: CK7 negativity was seen in 13% of Grade 3, 9% of Grade 2, and 2% of Grade 1 cases (P = 0.0457). Similarly, 28% of Grade 3, 5% of Grade 2 and 2% of Grade 1 cases were GATA3 negative (P < 0.0001). CK7 negative tumors did not show association with other clinicopathological parameters. GATA3 negative tumors were enriched in the basal-like molecular subgroup and were associated with negative estrogen receptor (ER) and negative progesterone receptor (PR) statuses. Both CK7 and GATA3 expression showed no association with overall survival in patients with Grade 3 tumor.Conclusions: This is the first study to characterize CK7 negative breast tumors in the context of clinicopathology. Profiling the CK7 negative and GATA3 negative breast cancers helps to understand the biology of these specific tumor subgroups and may aid in their diagnosis. [ABSTRACT FROM AUTHOR]

المؤلفون: Walter, Otto, Prasad, Manju, Lu, Shaolei, Quinlan, Robert M., Edmiston, Kathryn L., Khan, Ashraf

المصدر: Human Pathology; Nov2009, Vol. 40 Issue 11, p1528-1533, 6p

مصطلحات موضوعية: BIOMARKERS, PHENOTYPES, BREAST cancer diagnosis, CARCINOEMBRYONIC antigen, MESSENGER RNA, IMMUNOCYTOCHEMISTRY, EPIDERMAL growth factor

مستخلص: Summary: IMP3, an oncofetal protein, is a member of the insulin-like growth factor-II (IGF-II) mRNA-binding protein family. Its relevance as a novel biomarker in lung, pancreatic, renal, and cervical adenocarcinoma was recently revealed. However, its role in breast carcinogenesis and tumor progression is not yet established. Basal-like carcinoma was initially identified by gene expression profiling. It accounts for 15% to 30% of all breast cancers. These tumors express basal epithelial markers including cytokeratin 5 but lack expression of the estrogen receptor, progesterone receptor, and human epidermal growth factor receptor 2 (HER2), therefore, are often referred to as triple negative breast cancer. They have been found to be associated with a worse overall and disease-free survival. In this retrospective study, we examined the IMP3 expression in invasive ductal carcinoma of the breast and correlated its expression with morphological and biologic prognostic factors. The study group comprised 138 cases of invasive ductal carcinoma retrieved from the surgical pathologic files for a 10-year period from 1997 to 2006. Survival data and clinical stage were available on all 138 patients. Tumor characteristics including size, grade, lymphovascular invasion, necrosis, lymph node metastasis, estrogen receptor, progesterone receptor, and HER2 status were obtained from pathologic reports. Immunohistochemistry was performed on formalin-fixed paraffin-embedded tissue using mouse monoclonal antibody against IMP3 and CK5/6. Of the 138 breast cancer cases, IMP3 expression was seen in 45 (33%). Twenty-five of the IMP3+ cases were triple negative. We found significant correlation between IMP3 expression and higher grade (P = .001), necrosis (P< .0001) triple negative, and CK5/6 expression (P < .0001 for each). Cox multivariate analysis showed a hazard ratio of IMP3 expression at 3.14 (P = .05). IMP3 is a novel biomarker for triple negative (basal-like) invasive mammary carcinoma, and its expression is associated with a more aggressive phenotype and decreased overall survival. [Copyright &y& Elsevier]

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