Embryogenesis induction, callogenesis, and plant regeneration by in vitro culture of tomato isolated microspores and whole anthers
| العنوان: | Embryogenesis induction, callogenesis, and plant regeneration by in vitro culture of tomato isolated microspores and whole anthers |
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| المؤلفون: | Jose M. Seguí-Simarro, Fernando Nuez |
| المصدر: | Journal of Experimental Botany. 58:1119-1132 |
| بيانات النشر: | Oxford University Press (OUP), 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Spores, Physiology, Callus formation, fungi, Stamen, Morphogenesis, Embryonic Development, food and beverages, Context (language use), Embryo, Organogenesis, Flowers, Plant Science, Haploidy, Biology, Tissue Culture Techniques, Solanum lycopersicum, Microspore, Callus, Botany, Regeneration |
| الوصف: | In this work, some of the different in vitro developmental pathways into which tomato microspores or microsporocytes can be deviated experimentally were explored. The two principal ones are direct embryogenesis from isolated microspores and callus formation from meiocyte-containing anthers. By means of light and electron microscopy, the process of early embryogenesis from isolated microspores and the disruption of normal meiotic development and change of developmental fate towards callus proliferation, morphogenesis, and plant regeneration have been shown. From microspores isolated at the vacuolate stage, embryos can be directly induced, thus avoiding non-androgenic products. In contrast, several different morphogenic events can be triggered in cultures of microsporocyte-containing anthers under adequate conditions, including indirect embryogenesis, adventitious organogenesis, and plant regeneration. Both callus and regenerated plants may be haploid, diploid, and mostly mixoploid. The results demonstrate that both gametophytic and sporophytic calli occur in cultured tomato anthers, and point to an in vitro-induced disturbance of cytokinesis and subsequent fusion of daughter nuclei as a putative cause for mixoploidy and genome doubling during both tetrad compartmentalization and callus proliferation. The potential implications of the different alternative pathways are discussed in the context of their application to the production of doubled-haploid plants in tomato, which is still very poorly developed. |
| تدمد: | 1460-2431 0022-0957 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d98d6145df62228e5908702b89d86a1fTest https://doi.org/10.1093/jxb/erl271Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d98d6145df62228e5908702b89d86a1f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14602431 00220957 |
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