التفاصيل البيبلوغرافية
| العنوان: |
Prostate cancer cells modulate osteoblast mineralisation and osteoclast differentiation through Id-1. |
| المؤلفون: |
Yuen, H.-F., Chiu, Y.-T., Chan, K.-K., Chan, Y.-P., Chua, C.-W., McCrudden, C. M., Tang, K.-H., El-Tanani, M., Wong, Y.-C., Wang, X., Chan, K.-W. |
| المصدر: |
British Journal of Cancer; 1/19/2010, Vol. 102 Issue 2, p332-341, 10p, 2 Charts, 6 Graphs |
| مصطلحات موضوعية: |
BONE metastasis, PROSTATE cancer, OSTEOCLASTS, CELLS, IMMUNOHISTOCHEMISTRY, CANCER patients, OSTEOBLAST metabolism, BONE growth, CELL differentiation, CELL lines, COMPARATIVE studies, MACROPHAGES, RESEARCH methodology, MEDICAL cooperation, METASTASIS, PROSTATE tumors, PROTEINS, RESEARCH, EVALUATION research |
| مستخلص: |
Background: Id-1 is overexpressed in and correlated with metastatic potential of prostate cancer. The role of Id-1 in this metastatic process was further analysed.Methods: Conditioned media from prostate cancer cells, expressing various levels of Id-1, were used to stimulate pre-osteoclast differentiation and osteoblast mineralisation. Downstream effectors of Id-1 were identified. Expressions of Id-1 and its downstream effectors in prostate cancers were studied using immunohistochemistry in a prostate cancer patient cohort (N=110).Results: We found that conditioned media from LNCaP prostate cancer cells overexpressing Id-1 had a higher ability to drive osteoclast differentiation and a lower ability to stimulate osteoblast mineralisation than control, whereas conditioned media from PC3 prostate cancer cells with Id-1 knockdown were less able to stimulate osteoclast differentiation. Id-1 was found to negatively regulate TNF-beta and this correlation was confirmed in human prostate cancer specimens (P=0.03). Furthermore, addition of recombinant TNF-beta to LNCaP Id-1 cell-derived media blocked the effect of Id-1 overexpression on osteoblast mineralisation.Conclusion: In prostate cancer cells, the ability of Id-1 to modulate bone cell differentiation favouring metastatic bone disease is partially mediated by TNF-beta, and Id-1 could be a potential therapeutic target for prostate cancer to bone metastasis. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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