المؤلفون: Geoffroy-Siraudin, Cendrine, Perrard, Marie-Hélène, Ghalamoun-Slaimi, Rahma, Ali, Sazan, Chaspoul, Florence, Lanteaume, Andre, Achard, Vincent, Gallice, Philippe, Durand, Philippe, Guichaoua, Marie-Roberte

المساهمون: Université de la Méditerranée - Aix-Marseille 2, Assistance Publique - Hôpitaux de Marseille (APHM), Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)

المصدر: ISSN: 0041-008X.

مصطلحات موضوعية: Cadmium, Rat, Spermatogenesis, IN-VITRO MODEL, BLOOD, SPERMATOCYTES, DISRUPTION, ACTIVATION, Synaptonemal complex, SYNAPTONEMAL COMPLEX DAMAGE, RAT TESTIS, SPERMATOGENIC CELLS, MEIOSIS, POPULATIONS, Culture, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]

الوصف: International audience ; Using a validated model of culture of rat seminiferous tubules, we assessed the effects of 0.1, 1 and 10 mu g/L cadmium (Cd) on spermatogenic cells over a 2-week culture period. With concentrations of 1 and 10 mu g/L in the culture medium, the Cd concentration in the cells, determined by ICP-MS, increased with concentration in the medium and the day of culture. Flow cytometric analysis enabled us to evaluate changes in the number of Sertoli cells and germ cells during the culture period. The number of Sertoli cells did not appear to be affected by Cd. By contrast, spermatogonia and meiotic cells were decreased by 1 and 10 mu g/L Cd in a time and dose dependent manner. Stage distribution of the meiotic prophase I and qualitative study of the synaptonemal complexes (SC) at the pachytene stage were performed by immunocytochemistry with an anti SCP3 antibody. Cd caused a time-and-dose-dependent increase of total abnormalities, of fragmented SC and of asynapsis from concentration of 0.1 mu g/L. Additionally, we observed a new SC abnormality, the "motheaten" SC. This abnormality is frequently associated with asynapsis and SC widening which increased with both the Cd concentration and the duration of exposure. This abnormality suggests that Cd disrupts the structure and function of proteins involved in pairing and/or meiotic recombination. These results show that Cd induces dose-and-time-dependent alterations of the meiotic process of spermatogenesis ex-vivo, and that the lowest metal concentration, which induces an adverse effect, may vary with the cell parameter studied. (C) 2012 Elsevier Inc. All rights reserved.

العلاقة: hal-02644732; https://hal.inrae.fr/hal-02644732Test; PRODINRA: 214384; WOS: 000306888800004

الإتاحة: https://doi.org/10.1016/j.taap.2012.04.033Test
https://hal.inrae.fr/hal-02644732Test

المؤلفون: Geoffroy-Siraudin, Cendrine, Perrard, Marie-Hélène, Ghalamoun-Slaimi, Rahma, Ali, Sazan, Chaspoul, Florence, Lanteaume, Andre, Achard, Vincent, Gallice, Philippe, Durand, Philippe, Guichaoua, Marie-Roberte

المساهمون: Université de la Méditerranée - Aix-Marseille 2, Assistance Publique - Hôpitaux de Marseille (APHM), Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)

المصدر: ISSN: 0041-008X.

مصطلحات موضوعية: Cadmium, Rat, Spermatogenesis, IN-VITRO MODEL, BLOOD, SPERMATOCYTES, DISRUPTION, ACTIVATION, Synaptonemal complex, SYNAPTONEMAL COMPLEX DAMAGE, RAT TESTIS, SPERMATOGENIC CELLS, MEIOSIS, POPULATIONS, Culture, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]

الوصف: International audience ; Using a validated model of culture of rat seminiferous tubules, we assessed the effects of 0.1, 1 and 10 mu g/L cadmium (Cd) on spermatogenic cells over a 2-week culture period. With concentrations of 1 and 10 mu g/L in the culture medium, the Cd concentration in the cells, determined by ICP-MS, increased with concentration in the medium and the day of culture. Flow cytometric analysis enabled us to evaluate changes in the number of Sertoli cells and germ cells during the culture period. The number of Sertoli cells did not appear to be affected by Cd. By contrast, spermatogonia and meiotic cells were decreased by 1 and 10 mu g/L Cd in a time and dose dependent manner. Stage distribution of the meiotic prophase I and qualitative study of the synaptonemal complexes (SC) at the pachytene stage were performed by immunocytochemistry with an anti SCP3 antibody. Cd caused a time-and-dose-dependent increase of total abnormalities, of fragmented SC and of asynapsis from concentration of 0.1 mu g/L. Additionally, we observed a new SC abnormality, the "motheaten" SC. This abnormality is frequently associated with asynapsis and SC widening which increased with both the Cd concentration and the duration of exposure. This abnormality suggests that Cd disrupts the structure and function of proteins involved in pairing and/or meiotic recombination. These results show that Cd induces dose-and-time-dependent alterations of the meiotic process of spermatogenesis ex-vivo, and that the lowest metal concentration, which induces an adverse effect, may vary with the cell parameter studied. (C) 2012 Elsevier Inc. All rights reserved.

العلاقة: hal-02644732; https://hal.inrae.fr/hal-02644732Test; PRODINRA: 214384; WOS: 000306888800004

الإتاحة: https://doi.org/10.1016/j.taap.2012.04.033Test
https://hal.inrae.fr/hal-02644732Test

المؤلفون: Geoffroy-Siraudin, Cendrine, Perrard, Marie-Hélène, Ghalamoun-Slaimi, Rahma, Ali, Sazan, Chaspoul, Florence, Lanteaume, Andre, Achard, Vincent, Gallice, Philippe, Durand, Philippe, Guichaoua, Marie-Roberte

المساهمون: Université de la Méditerranée - Aix-Marseille 2, Assistance Publique - Hôpitaux de Marseille (APHM), Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)

المصدر: ISSN: 0041-008X.

مصطلحات موضوعية: Cadmium, Rat, Spermatogenesis, IN-VITRO MODEL, BLOOD, SPERMATOCYTES, DISRUPTION, ACTIVATION, Synaptonemal complex, SYNAPTONEMAL COMPLEX DAMAGE, RAT TESTIS, SPERMATOGENIC CELLS, MEIOSIS, POPULATIONS, Culture, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]

الوصف: International audience ; Using a validated model of culture of rat seminiferous tubules, we assessed the effects of 0.1, 1 and 10 mu g/L cadmium (Cd) on spermatogenic cells over a 2-week culture period. With concentrations of 1 and 10 mu g/L in the culture medium, the Cd concentration in the cells, determined by ICP-MS, increased with concentration in the medium and the day of culture. Flow cytometric analysis enabled us to evaluate changes in the number of Sertoli cells and germ cells during the culture period. The number of Sertoli cells did not appear to be affected by Cd. By contrast, spermatogonia and meiotic cells were decreased by 1 and 10 mu g/L Cd in a time and dose dependent manner. Stage distribution of the meiotic prophase I and qualitative study of the synaptonemal complexes (SC) at the pachytene stage were performed by immunocytochemistry with an anti SCP3 antibody. Cd caused a time-and-dose-dependent increase of total abnormalities, of fragmented SC and of asynapsis from concentration of 0.1 mu g/L. Additionally, we observed a new SC abnormality, the "motheaten" SC. This abnormality is frequently associated with asynapsis and SC widening which increased with both the Cd concentration and the duration of exposure. This abnormality suggests that Cd disrupts the structure and function of proteins involved in pairing and/or meiotic recombination. These results show that Cd induces dose-and-time-dependent alterations of the meiotic process of spermatogenesis ex-vivo, and that the lowest metal concentration, which induces an adverse effect, may vary with the cell parameter studied. (C) 2012 Elsevier Inc. All rights reserved.

العلاقة: hal-02644732; https://hal.inrae.fr/hal-02644732Test; PRODINRA: 214384; WOS: 000306888800004

الإتاحة: https://doi.org/10.1016/j.taap.2012.04.033Test
https://hal.inrae.fr/hal-02644732Test

المؤلفون: Geoffroy-Siraudin, Cendrine, Perrard, Marie-Hélène, Ghalamoun-Slaimi, Rahma, Ali, Sazan, Chaspoul, Florence, Lanteaume, Andre, Achard, Vincent, Gallice, Philippe, Durand, Philippe, Guichaoua, Marie-Roberte

المساهمون: Université de la Méditerranée - Aix-Marseille 2, Assistance Publique - Hôpitaux de Marseille (APHM), Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)

المصدر: ISSN: 0041-008X.

مصطلحات موضوعية: Cadmium, Rat, Spermatogenesis, IN-VITRO MODEL, BLOOD, SPERMATOCYTES, DISRUPTION, ACTIVATION, Synaptonemal complex, SYNAPTONEMAL COMPLEX DAMAGE, RAT TESTIS, SPERMATOGENIC CELLS, MEIOSIS, POPULATIONS, Culture, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]

الوصف: International audience ; Using a validated model of culture of rat seminiferous tubules, we assessed the effects of 0.1, 1 and 10 mu g/L cadmium (Cd) on spermatogenic cells over a 2-week culture period. With concentrations of 1 and 10 mu g/L in the culture medium, the Cd concentration in the cells, determined by ICP-MS, increased with concentration in the medium and the day of culture. Flow cytometric analysis enabled us to evaluate changes in the number of Sertoli cells and germ cells during the culture period. The number of Sertoli cells did not appear to be affected by Cd. By contrast, spermatogonia and meiotic cells were decreased by 1 and 10 mu g/L Cd in a time and dose dependent manner. Stage distribution of the meiotic prophase I and qualitative study of the synaptonemal complexes (SC) at the pachytene stage were performed by immunocytochemistry with an anti SCP3 antibody. Cd caused a time-and-dose-dependent increase of total abnormalities, of fragmented SC and of asynapsis from concentration of 0.1 mu g/L. Additionally, we observed a new SC abnormality, the "motheaten" SC. This abnormality is frequently associated with asynapsis and SC widening which increased with both the Cd concentration and the duration of exposure. This abnormality suggests that Cd disrupts the structure and function of proteins involved in pairing and/or meiotic recombination. These results show that Cd induces dose-and-time-dependent alterations of the meiotic process of spermatogenesis ex-vivo, and that the lowest metal concentration, which induces an adverse effect, may vary with the cell parameter studied. (C) 2012 Elsevier Inc. All rights reserved.

العلاقة: hal-02644732; https://hal.inrae.fr/hal-02644732Test; PRODINRA: 214384; WOS: 000306888800004

الإتاحة: https://doi.org/10.1016/j.taap.2012.04.033Test
https://hal.inrae.fr/hal-02644732Test

المؤلفون: Geoffroy-Siraudin, Cendrine, Perrard, Marie-Hélène, Ghalamoun-Slaimi, Rahma, Ali, Sazan, Chaspoul, Florence, Lanteaume, Andre, Achard, Vincent, Gallice, Philippe, Durand, Philippe, Guichaoua, Marie-Roberte

المساهمون: Université de la Méditerranée - Aix-Marseille 2, Assistance Publique - Hôpitaux de Marseille (APHM), Institut de Génomique Fonctionnelle de Lyon (IGFL), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Lyon (ENS Lyon)

المصدر: ISSN: 0041-008X.

مصطلحات موضوعية: Cadmium, Rat, Spermatogenesis, IN-VITRO MODEL, BLOOD, SPERMATOCYTES, DISRUPTION, ACTIVATION, Culture, Meiosis, Synaptonemal complex, SYNAPTONEMAL COMPLEX DAMAGE, RAT TESTIS, SPERMATOGENIC CELLS, POPULATIONS, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]

الوصف: International audience ; Using a validated model of culture of rat seminiferous tubules, we assessed the effects of 0.1, 1 and 10 mu g/L cadmium (Cd) on spermatogenic cells over a 2-week culture period. With concentrations of 1 and 10 mu g/L in the culture medium, the Cd concentration in the cells, determined by ICP-MS, increased with concentration in the medium and the day of culture. Flow cytometric analysis enabled us to evaluate changes in the number of Sertoli cells and germ cells during the culture period. The number of Sertoli cells did not appear to be affected by Cd. By contrast, spermatogonia and meiotic cells were decreased by 1 and 10 mu g/L Cd in a time and dose dependent manner. Stage distribution of the meiotic prophase I and qualitative study of the synaptonemal complexes (SC) at the pachytene stage were performed by immunocytochemistry with an anti SCP3 antibody. Cd caused a time-and-dose-dependent increase of total abnormalities, of fragmented SC and of asynapsis from concentration of 0.1 mu g/L. Additionally, we observed a new SC abnormality, the "motheaten" SC. This abnormality is frequently associated with asynapsis and SC widening which increased with both the Cd concentration and the duration of exposure. This abnormality suggests that Cd disrupts the structure and function of proteins involved in pairing and/or meiotic recombination. These results show that Cd induces dose-and-time-dependent alterations of the meiotic process of spermatogenesis ex-vivo, and that the lowest metal concentration, which induces an adverse effect, may vary with the cell parameter studied. (C) 2012 Elsevier Inc. All rights reserved.

العلاقة: hal-02644732; https://hal.inrae.fr/hal-02644732Test; PRODINRA: 214384; WOS: 000306888800004

الإتاحة: https://doi.org/10.1016/j.taap.2012.04.033Test
https://hal.inrae.fr/hal-02644732Test