التفاصيل البيبلوغرافية
| العنوان: |
Purification and Initial Characterization of Ara h 7, a Peanut Allergen from the 2S Albumin Protein Family |
| المؤلفون: |
Danijela Apostolovic (4474696), Justin T. Marsh (2175010), Joe Baumert (10879177), Steve L. Taylor (1712011), Adrie Westphal (3451130), Harmen de Jongh (10879180), Phil Johnson (2183350), Govardus A.H. de Jong (10879183), Stef J. Koppelman (10879186) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biochemistry, Cell Biology, Genetics, Molecular Biology, Pharmacology, Biotechnology, Immunology, Cancer, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, Ara h 6, Ara h 7, post-translation cleavage, Ara h 2, mass spectrometry, Peanut Allergen, Initial Characterization, market types, peanut 2 S albumin protein family, disulfide bonds, cross-reactive IgE epitopes, Ara h 7 isoforms, hydroxyproline modification, 2 S Albumin Protein Family 2 S albumins, peanut allergens, Ara h 7 isoforms bind IgE, protein family |
| الوصف: |
2S albumins are important peanut allergens. Within this protein family, Ara h 2 and Ara h 6 have been described in detail, but Ara h 7 has received little attention. We now describe the first purification of Ara h 7 and its characterization. Two Ara h 7 isoforms were purified from peanuts. Mass spectrometry revealed that both the isoforms have a post-translation cleavage, a hydroxyproline modification near the N -terminus, and four disulfide bonds. The secondary structure of both Ara h 7 isoforms is highly comparable to those of Ara h 2 and Ara h 6. Both Ara h 7 isoforms bind IgE, and Ara h 7 is capable of inhibiting the binding between Ara h 2 and IgE, suggesting at least partially cross-reactive IgE epitopes. Ara h 7 was found in all main market types of peanut, at comparable levels. This suggests that Ara h 7 is a relevant allergen from the peanut 2S albumin protein family. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/journal_contribution/Purification_and_Initial_Characterization_of_Ara_h_7_a_Peanut_Allergen_from_the_2S_Albumin_Protein_Family/14681407Test |
| DOI: |
10.1021/acs.jafc.1c00618.s001 |
| الإتاحة: |
https://doi.org/10.1021/acs.jafc.1c00618.s001Test |
| حقوق: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.9FA75493 |
| قاعدة البيانات: |
BASE |
