Multiple quantitative trait loci contribute to resistance to bacterial canker incited by Pseudomonas syringae pv. actinidiae in kiwifruit (Actinidia chinensis)
| العنوان: | Multiple quantitative trait loci contribute to resistance to bacterial canker incited by Pseudomonas syringae pv. actinidiae in kiwifruit (Actinidia chinensis) |
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| المؤلفون: | Ross N. Crowhurst, Keith A. Funnell, Stephen Hoyte, Luis Gea, Abhishek Chatterjee, David Chagné, Cecilia H. Deng, Joel L. Vanneste, Amardeep Nath, Ed R. Morgan, H. Bassett, Cyril Brendolise, Mirco Montefiori, Mareike Knaebel, Andrew Wotton, Susan E. Gardiner, Kerry Robert Templeton, Duncan Hedderley, Jibran Tahir, John McCallum, Claudia Wiedow, Kirsten Hoeata |
| المصدر: | Horticulture Research Horticulture Research, Vol 6, Iss 1, Pp 1-18 (2019) |
| بيانات النشر: | Oxford University Press (OUP), 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Actinidia chinensis, Population, Plant Science, Horticulture, Quantitative trait locus, urologic and male genital diseases, 01 natural sciences, Biochemistry, Article, 03 medical and health sciences, lcsh:Botany, Genotype, Genetics, Pseudomonas syringae, Allele, education, lcsh:QH301-705.5, education.field_of_study, Virulence, biology, Actinidia, food and beverages, biology.organism_classification, lcsh:QK1-989, 030104 developmental biology, lcsh:Biology (General), Genetic marker, Genetic markers, 010606 plant biology & botany, Biotechnology |
| الوصف: | Pseudomonas syringae pv. actinidiae (Psa) biovar 3, a virulent, canker-inducing pathogen is an economic threat to the kiwifruit (Actinidia spp.) industry worldwide. The commercially grown diploid (2×) A. chinensis var. chinensis is more susceptible to Psa than tetraploid and hexaploid kiwifruit. However information on the genetic loci modulating Psa resistance in kiwifruit is not available. Here we report mapping of quantitative trait loci (QTLs) regulating resistance to Psa in a diploid kiwifruit population, derived from a cross between an elite Psa-susceptible ‘Hort16A’ and a resistant male breeding parent P1. Using high-density genetic maps and intensive phenotyping, we identified a single QTL for Psa resistance on Linkage Group (LG) 27 of ‘Hort16A’ revealing 16–19% phenotypic variance and candidate alleles for susceptibility and resistance at this loci. In addition, six minor QTLs were identified in P1 on distinct LGs, exerting 4–9% variance. Resistance in the F1 population is improved by additive effects from ‘Hort16A’ and P1 QTLs providing evidence that divergent genetic pathways interact to combat the virulent Psa strain. Two different bioassays further identified new QTLs for tissue-specific responses to Psa. The genetic marker at LG27 QTL was further verified for association with Psa resistance in diploid Actinidia chinensis populations. Transcriptome analysis of Psa-resistant and susceptible genotypes in field revealed hallmarks of basal defense and provided candidate RNA-biomarkers for screening for Psa resistance in greenhouse conditions. |
| تدمد: | 2052-7276 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d83579cf6d3f69d126d7b491178ed182Test https://doi.org/10.1038/s41438-019-0184-9Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d83579cf6d3f69d126d7b491178ed182 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20527276 |
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