Sexual reproduction and mating-type–mediated strain development in the penicillin-producing fungus Penicillium chrysogenum
| العنوان: | Sexual reproduction and mating-type–mediated strain development in the penicillin-producing fungus Penicillium chrysogenum |
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| المؤلفون: | Hubert Kürnsteiner, Julia Böhm, Volker Klix, Stefanie Pöggeler, Ivo Zadra, Ulrich Kück, Céline M. O’Gorman, Simon Wolfers, Danielle Binger, Birgit Hoff, Paul S. Dyer |
| المصدر: | Proceedings of the National Academy of Sciences. 110:1476-1481 |
| بيانات النشر: | Proceedings of the National Academy of Sciences, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Mating type, Gene Expression, Penicillins, Fungus, Penicillium chrysogenum, Pheromones, Microbiology, Fungal Proteins, 03 medical and health sciences, Meiosis, Heterothallic, DNA, Fungal, Gene, Crosses, Genetic, 030304 developmental biology, Recombination, Genetic, 0303 health sciences, Fungal protein, Multidisciplinary, Base Sequence, biology, 030306 microbiology, Reproduction, Biological Sciences, Spores, Fungal, Genes, Mating Type, Fungal, biology.organism_classification, Receptors, Pheromone, Sexual reproduction, Phenotype, Gene Deletion, Biotechnology |
| الوصف: | Penicillium chrysogenum is a filamentous fungus of major medical and historical importance, being the original and present-day industrial source of the antibiotic penicillin. The species has been considered asexual for more than 100 y, and despite concerted efforts, it has not been possible to induce sexual reproduction, which has prevented sexual crosses being used for strain improvement. However, using knowledge of mating-type ( MAT ) gene organization, we now describe conditions under which a sexual cycle can be induced leading to production of meiotic ascospores. Evidence of recombination was obtained using both molecular and phenotypic markers. The identified heterothallic sexual cycle was used for strain development purposes, generating offspring with novel combinations of traits relevant to penicillin production. Furthermore, the MAT1-1–1 mating-type gene, known primarily for a role in governing sexual identity, was also found to control transcription of a wide range of genes with biotechnological relevance including those regulating penicillin production, hyphal morphology, and conidial formation. These discoveries of a sexual cycle and MAT gene function are likely to be of broad relevance for manipulation of other asexual fungi of economic importance. |
| وصف الملف: | application/pdf |
| تدمد: | 1091-6490 0027-8424 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::584ac87fca7de1b41092d883a088e5d1Test https://doi.org/10.1073/pnas.1217943110Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....584ac87fca7de1b41092d883a088e5d1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10916490 00278424 |
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