-
1دورية أكاديمية
المؤلفون: Dongmin Kim, Terry J. DeBriere, Bradley H. Eastmond, Abdullah A. Alomar, Ozlem Yaren, Jacquelyn McCarter, Kevin M. Bradley, Steven A. Benner, Barry W. Alto, Nathan D. Burkett-Cadena
مصطلحات موضوعية: Medicine, Molecular Biology, Biotechnology, Ecology, Cancer, Mental Health, Infectious Diseases, Virology,
+arthropod%22">xlink "> arthropod, within 45 minutes, vector control responses, sentinel chicken serosurveillance, permitting arbovirus monitoring, negative samples based, monitor arbovirus transmission, mediated isothermal amplification, least 48 hours, feed directly upon, elicits infectious mosquitoes, early warning value, borne pathogen surveillance, mosquito pool screening, laborious mosquito identification, without rna extraction, infectious mosquito saliva, mosquito saliva, borne viruses, west nile, virus isolation, thermal treatment الوصف: Colorimetric values, including hue (H-value), saturation (S-value), and brightness (B-value), at the endpoint of DP-LAMP products with or without sunlight exposure at various viral titers (i.e., 2, 3, 4, and 5 log 10 PFU) over time points (i.e., 0, 15, 30, and 60 min) were quantified using blue LED and orange filter and OpenCV with Python.
الإتاحة: https://doi.org/10.1371/journal.pone.0298805.s008Test
https://figshare.com/articles/journal_contribution/Colorimetric_values_including_hue_H-value_saturation_S-value_and_brightness_B-value_at_the_endpoint_of_DP-LAMP_products_with_or_without_sunlight_exposure_at_various_viral_titers_i_e_2_3_4_and_5_log_sub_10_sub_PFU_over_time_points_i_e_0_15_3/25280072Test -
2دورية أكاديمية
المؤلفون: Dongmin Kim, Terry J. DeBriere, Bradley H. Eastmond, Abdullah A. Alomar, Ozlem Yaren, Jacquelyn McCarter, Kevin M. Bradley, Steven A. Benner, Barry W. Alto, Nathan D. Burkett-Cadena
مصطلحات موضوعية: Medicine, Molecular Biology, Biotechnology, Ecology, Cancer, Mental Health, Infectious Diseases, Virology,
+arthropod%22">xlink "> arthropod, within 45 minutes, vector control responses, sentinel chicken serosurveillance, permitting arbovirus monitoring, negative samples based, monitor arbovirus transmission, mediated isothermal amplification, least 48 hours, feed directly upon, elicits infectious mosquitoes, early warning value, borne pathogen surveillance, mosquito pool screening, laborious mosquito identification, without rna extraction, infectious mosquito saliva, mosquito saliva, borne viruses, west nile, virus isolation, thermal treatment الوصف: P = positive; N = negative. N/A = not applicable. (DOCX)
الإتاحة: https://doi.org/10.1371/journal.pone.0298805.s006Test
https://figshare.com/articles/journal_contribution/Results_of_DP-LAMP_and_RT-qPCR_for_West_Nile_virus_WNV_and_Dengue-I_virus_DENV-I_detection_from_mosquito_saliva_/25280066Test -
3دورية أكاديمية
المؤلفون: Dongmin Kim, Terry J. DeBriere, Bradley H. Eastmond, Abdullah A. Alomar, Ozlem Yaren, Jacquelyn McCarter, Kevin M. Bradley, Steven A. Benner, Barry W. Alto, Nathan D. Burkett-Cadena
مصطلحات موضوعية: Medicine, Molecular Biology, Biotechnology, Ecology, Cancer, Mental Health, Infectious Diseases, Virology,
+arthropod%22">xlink "> arthropod, within 45 minutes, vector control responses, sentinel chicken serosurveillance, permitting arbovirus monitoring, negative samples based, monitor arbovirus transmission, mediated isothermal amplification, least 48 hours, feed directly upon, elicits infectious mosquitoes, early warning value, borne pathogen surveillance, mosquito pool screening, laborious mosquito identification, without rna extraction, infectious mosquito saliva, mosquito saliva, borne viruses, west nile, virus isolation, thermal treatment الوصف: The DP-LAMP primers and strand-displaceable probes for DENV-I were purchased from IDT [ 24 ]. The WNV primer set was developed based on multiple sequences, utilizing 85 full genome sequences of WNV Subtype 1a obtained from human hosts and retrieved from viprbrc.org. (DOCX)
الإتاحة: https://doi.org/10.1371/journal.pone.0298805.s005Test
https://figshare.com/articles/journal_contribution/DP-LAMP_primer_and_probe_sequences_used_in_this_study_/25280063Test -
4دورية أكاديمية
المؤلفون: Dongmin Kim, Terry J. DeBriere, Bradley H. Eastmond, Abdullah A. Alomar, Ozlem Yaren, Jacquelyn McCarter, Kevin M. Bradley, Steven A. Benner, Barry W. Alto, Nathan D. Burkett-Cadena
مصطلحات موضوعية: Medicine, Molecular Biology, Biotechnology, Ecology, Cancer, Mental Health, Infectious Diseases, Virology,
+arthropod%22">xlink "> arthropod, within 45 minutes, vector control responses, sentinel chicken serosurveillance, permitting arbovirus monitoring, negative samples based, monitor arbovirus transmission, mediated isothermal amplification, least 48 hours, feed directly upon, elicits infectious mosquitoes, early warning value, borne pathogen surveillance, mosquito pool screening, laborious mosquito identification, without rna extraction, infectious mosquito saliva, mosquito saliva, borne viruses, west nile, virus isolation, thermal treatment الوصف: Tubes with reagents with WNV at various viral titers (i.e., 2, 3, 4, and 5 log 10 PFU) were exposed to sunlight over time (i.e., 0, 15, 30, and 60 min) and then visualized using blue LED and orange filter. Tubes without virus (letter “N”) were included as negative controls. The letter “P” indicates tubes with WNV. (DOCX)
الإتاحة: https://doi.org/10.1371/journal.pone.0298805.s007Test
https://figshare.com/articles/journal_contribution/Effect_of_sunlight_exposure_on_stability_and_visualization_of_DP-LAMP_products_/25280069Test -
5دورية أكاديمية
المؤلفون: Dongmin Kim, Terry J. DeBriere, Bradley H. Eastmond, Abdullah A. Alomar, Ozlem Yaren, Jacquelyn McCarter, Kevin M. Bradley, Steven A. Benner, Barry W. Alto, Nathan D. Burkett-Cadena
مصطلحات موضوعية: Medicine, Molecular Biology, Biotechnology, Ecology, Cancer, Mental Health, Infectious Diseases, Virology,
+arthropod%22">xlink "> arthropod, within 45 minutes, vector control responses, sentinel chicken serosurveillance, permitting arbovirus monitoring, negative samples based, monitor arbovirus transmission, mediated isothermal amplification, least 48 hours, feed directly upon, elicits infectious mosquitoes, early warning value, borne pathogen surveillance, mosquito pool screening, laborious mosquito identification, without rna extraction, infectious mosquito saliva, mosquito saliva, borne viruses, west nile, virus isolation, thermal treatment الوصف: The DP-LAMP primers and strand-displaceable probes for DENV-I were purchased from IDT [ 24 ]. The WNV primer set was developed based on multiple sequences, utilizing 85 full genome sequences of WNV Subtype 1a obtained from human hosts and retrieved from viprbrc.org. (DOCX)
-
6دورية أكاديمية
المؤلفون: Dongmin Kim, Terry J. DeBriere, Bradley H. Eastmond, Abdullah A. Alomar, Ozlem Yaren, Jacquelyn McCarter, Kevin M. Bradley, Steven A. Benner, Barry W. Alto, Nathan D. Burkett-Cadena
مصطلحات موضوعية: Medicine, Molecular Biology, Biotechnology, Ecology, Cancer, Mental Health, Infectious Diseases, Virology,
+arthropod%22">xlink "> arthropod, within 45 minutes, vector control responses, sentinel chicken serosurveillance, permitting arbovirus monitoring, negative samples based, monitor arbovirus transmission, mediated isothermal amplification, least 48 hours, feed directly upon, elicits infectious mosquitoes, early warning value, borne pathogen surveillance, mosquito pool screening, laborious mosquito identification, without rna extraction, infectious mosquito saliva, mosquito saliva, borne viruses, west nile, virus isolation, thermal treatment الوصف: P = positive; N = negative. N/A = not applicable. (DOCX)
-
7دورية أكاديمية
المؤلفون: Dongmin Kim, Terry J. DeBriere, Bradley H. Eastmond, Abdullah A. Alomar, Ozlem Yaren, Jacquelyn McCarter, Kevin M. Bradley, Steven A. Benner, Barry W. Alto, Nathan D. Burkett-Cadena
مصطلحات موضوعية: Medicine, Molecular Biology, Biotechnology, Ecology, Cancer, Mental Health, Infectious Diseases, Virology,
+arthropod%22">xlink "> arthropod, within 45 minutes, vector control responses, sentinel chicken serosurveillance, permitting arbovirus monitoring, negative samples based, monitor arbovirus transmission, mediated isothermal amplification, least 48 hours, feed directly upon, elicits infectious mosquitoes, early warning value, borne pathogen surveillance, mosquito pool screening, laborious mosquito identification, without rna extraction, infectious mosquito saliva, mosquito saliva, borne viruses, west nile, virus isolation, thermal treatment الوصف: Tubes with reagents with WNV at various viral titers (i.e., 2, 3, 4, and 5 log 10 PFU) were exposed to sunlight over time (i.e., 0, 15, 30, and 60 min) and then visualized using blue LED and orange filter. Tubes without virus (letter “N”) were included as negative controls. The letter “P” indicates tubes with WNV. (DOCX)
-
8دورية أكاديمية
المؤلفون: Dongmin Kim, Terry J. DeBriere, Bradley H. Eastmond, Abdullah A. Alomar, Ozlem Yaren, Jacquelyn McCarter, Kevin M. Bradley, Steven A. Benner, Barry W. Alto, Nathan D. Burkett-Cadena
مصطلحات موضوعية: Medicine, Molecular Biology, Biotechnology, Ecology, Cancer, Mental Health, Infectious Diseases, Virology,
+arthropod%22">xlink "> arthropod, within 45 minutes, vector control responses, sentinel chicken serosurveillance, permitting arbovirus monitoring, negative samples based, monitor arbovirus transmission, mediated isothermal amplification, least 48 hours, feed directly upon, elicits infectious mosquitoes, early warning value, borne pathogen surveillance, mosquito pool screening, laborious mosquito identification, without rna extraction, infectious mosquito saliva, mosquito saliva, borne viruses, west nile, virus isolation, thermal treatment الوصف: Colorimetric values, including hue (H-value), saturation (S-value), and brightness (B-value), at the endpoint of DP-LAMP products with or without sunlight exposure at various viral titers (i.e., 2, 3, 4, and 5 log 10 PFU) over time points (i.e., 0, 15, 30, and 60 min) were quantified using blue LED and orange filter and OpenCV with Python.
-
9صورة
المؤلفون: Dongmin Kim, Terry J. DeBriere, Bradley H. Eastmond, Abdullah A. Alomar, Ozlem Yaren, Jacquelyn McCarter, Kevin M. Bradley, Steven A. Benner, Barry W. Alto, Nathan D. Burkett-Cadena
مصطلحات موضوعية: Medicine, Molecular Biology, Biotechnology, Ecology, Cancer, Mental Health, Infectious Diseases, Virology,
+arthropod%22">xlink "> arthropod, within 45 minutes, vector control responses, sentinel chicken serosurveillance, permitting arbovirus monitoring, negative samples based, monitor arbovirus transmission, mediated isothermal amplification, least 48 hours, feed directly upon, elicits infectious mosquitoes, early warning value, borne pathogen surveillance, mosquito pool screening, laborious mosquito identification, without rna extraction, infectious mosquito saliva, mosquito saliva, borne viruses, west nile, virus isolation, thermal treatment الوصف: Sensitivity of the DP-LAMP and RT-qPCR for detection of WNV (A) and DENV-I (B).
الإتاحة: https://doi.org/10.1371/journal.pone.0298805.s002Test
https://figshare.com/articles/figure/Sensitivity_of_the_DP-LAMP_and_RT-qPCR_for_detection_of_WNV_A_and_DENV-I_B_/25280054Test -
10صورة
المؤلفون: Dongmin Kim, Terry J. DeBriere, Bradley H. Eastmond, Abdullah A. Alomar, Ozlem Yaren, Jacquelyn McCarter, Kevin M. Bradley, Steven A. Benner, Barry W. Alto, Nathan D. Burkett-Cadena
مصطلحات موضوعية: Medicine, Molecular Biology, Biotechnology, Ecology, Cancer, Mental Health, Infectious Diseases, Virology,
+arthropod%22">xlink "> arthropod, within 45 minutes, vector control responses, sentinel chicken serosurveillance, permitting arbovirus monitoring, negative samples based, monitor arbovirus transmission, mediated isothermal amplification, least 48 hours, feed directly upon, elicits infectious mosquitoes, early warning value, borne pathogen surveillance, mosquito pool screening, laborious mosquito identification, without rna extraction, infectious mosquito saliva, mosquito saliva, borne viruses, west nile, virus isolation, thermal treatment الوصف: Pros and cons of different methods used for arbovirus surveillance.
الإتاحة: https://doi.org/10.1371/journal.pone.0298805.s001Test
https://figshare.com/articles/figure/Pros_and_cons_of_different_methods_used_for_arbovirus_surveillance_/25280051Test