Microsomal preparations from elicited heterotrophic cell cultures of chickpea in the presence of O 2 and NADPH catalyse methylenedioxy bridge formation from pratensein (3′-OH-biochanin A) and calycosin (3′-OH-formononetin). The enzymatic activities are inhibited by CO and various P450 inhibitors and are therefore characterized as cytochrome P450-dependent.