دورية أكاديمية
Varicella-zoster virus infection of adult rat sensory neurons in vitro.
| العنوان: | Varicella-zoster virus infection of adult rat sensory neurons in vitro. |
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| المؤلفون: | Merville-Louis, M. P., Sadzot-Delvaux, Catherine, Delree, P., Piette, Jacques, Moonen, Gustave, Rentier, Bernard |
| المصدر: | Journal of Virology, 63 (7), 3155-60 (1989) |
| بيانات النشر: | American Society for Microbiology (ASM) |
| سنة النشر: | 1989 |
| المجموعة: | University of Liège: ORBi (Open Repository and Bibliography) |
| مصطلحات موضوعية: | Animals, Antibodies, Monoclonal, Cells, Cultured, DNA, Viral/analysis, Fluorescent Antibody Technique, Ganglia, Spinal/microbiology, Herpesvirus 3, Human/genetics/pathogenicity, Immunoenzyme Techniques, Neurons, Afferent/microbiology, RNA, Rats, Inbred Strains, Life sciences, Biochemistry, biophysics & molecular biology, Sciences du vivant, Biochimie, biophysique & biologie moléculaire |
| الوصف: | peer reviewed ; We report here an in vitro model of neuronal infection by varicella-zoster virus (VZV). Such a model has been achieved by using dissociated adult rat dorsal root ganglia cells infected by cocultivation with VZV-infected MRC5 cells or with cell-free virus. Indirect VZV immunolabeling, in situ hybridization, and neuron-specific immunolabeling demonstrated that VZV infection occurred selectively in neurons. VZV-specific immunolabeling detected a few neurons 1 or 2 days postinfection but not later. Genome detection using cloned VZV DNA probes revealed a hybridization signal primarily with RNA. Within 1 to 6 days postinfection, a progressive increase of VZV-specific hybridization was observed in up to 50% of the neurons. RNAs corresponding to immediate-early, early, and late genes were found, and transcripts of immediate-early gene 63 were particularly abundant. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0022-538X 1098-5514 |
| العلاقة: | urn:issn:0022-538X; urn:issn:1098-5514; https://orbi.uliege.be/handle/2268/33874Test; info:hdl:2268/33874; https://orbi.uliege.be/bitstream/2268/33874/1/Merville-louis%201989.pdfTest; scopus-id:2-s2.0-0024335710; info:pmid:2542622 |
| DOI: | 10.1128/jvi.63.7.3155-3160.1989 |
| الإتاحة: | https://doi.org/10.1128/jvi.63.7.3155-3160.1989Test https://orbi.uliege.be/handle/2268/33874Test https://orbi.uliege.be/bitstream/2268/33874/1/Merville-louis%201989.pdfTest |
| حقوق: | open access ; http://purl.org/coar/access_right/c_abf2Test ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.9B7459AE |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 0022538X 10985514 |
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| DOI: | 10.1128/jvi.63.7.3155-3160.1989 |