Epigenetic-scale comparison of human iPSCs generated by retrovirus, Sendai virus or episomal vectors
| العنوان: | Epigenetic-scale comparison of human iPSCs generated by retrovirus, Sendai virus or episomal vectors |
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| المؤلفون: | Mahito Nakanishi, Manami Ohtaka, Koichiro Nishino, Masashi Toyoda, Hidenori Akutsu, Tohru Sugawara, Yoshikazu Arai, Mayu Yamazaki-Inoue, Akihiro Umezawa, Ken Takasawa, Ken Nishimura |
| المصدر: | Regenerative Therapy Regenerative Therapy, Vol 9, Iss, Pp 71-78 (2018) |
| بيانات النشر: | Cold Spring Harbor Laboratory, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Human iPSCs, Biomedical Engineering, Biomaterials, 03 medical and health sciences, Retrovirus, SOX2, Epigenetics, lcsh:QH573-671, Aberrant methylation, Induced pluripotent stem cell, lcsh:R5-920, DNA methylation, biology, lcsh:Cytology, Human ESCs, biology.organism_classification, Embryonic stem cell, Sendai virus, Cell biology, 030104 developmental biology, Original Article, lcsh:Medicine (General), Reprogramming, Developmental Biology |
| الوصف: | Human induced pluripotent stem cells (iPSCs) are established by introducing several reprogramming factors, such as OCT3/4, SOX2, KLF4, c-MYC. Because of their pluripotency and immortality, iPSCs are considered to be a powerful tool for regenerative medicine. To date, iPSCs have been established all over the world by various gene delivery methods. All methods induced high-quality iPSCs, but epigenetic analysis of abnormalities derived from differences in the gene delivery methods has not yet been performed. Here, we generated genetically matched human iPSCs from menstrual blood cells by using three kinds of vectors, i.e., retrovirus, Sendai virus, and episomal vectors, and compared genome-wide DNA methylation profiles among them. Although comparison of aberrant methylation revealed that iPSCs generated by Sendai virus vector have lowest number of aberrant methylation sites among the three vectors, the iPSCs generated by non-integrating methods did not show vector-specific aberrant methylation. However, the differences between the iPSC lines were determined to be the number of random aberrant hyper-methylated regions compared with embryonic stem cells. These random aberrant hyper-methylations might be a cause of the differences in the properties of each of the iPSC lines. |
| وصف الملف: | application/pdf |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8b9157e1b981a74af739f0bafe06369cTest https://doi.org/10.1101/355099Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....8b9157e1b981a74af739f0bafe06369c |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |