Discrimination of agonist and antagonist forms of CXCL10 in biological samples
| العنوان: | Discrimination of agonist and antagonist forms of CXCL10 in biological samples |
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| المؤلفون: | M. Schmolz, Stanislas Pol, L. Stephen, Matthew L. Albert, James Mapes, Armanda Casrouge, Vincent Mallet, C. Pfister, A. Bisiaux |
| المساهمون: | Immunobiologie des Cellules Dendritiques, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Myriad rules based medicine, CHU Rouen, Normandie Université (NU), Service d'hépatologie médicale [CHU Cochin], Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre d'Immunologie Humaine (CIH), The work was funded by grants obtained from L'Institut National du Cancer (INCA), the European Research Council (ERC) and a private donation from Caisse de Retraite et de Prévoyance des Clercs et Employés de Notaires (CRPCEN)., The authors would like to thank members of the Centre d'Immunology Humaine (CIH) and the Pôle Integré de Recherche Clinique (PIRC) Institut Pasteur, and the members of the Liver Unit, Hopital Cochin for support of this work., Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Assistance publique - Hôpitaux de Paris (AP-HP)-CHU Cochin [AP-HP], Centre d'Immunologie Humaine ( CIH ) |
| المصدر: | Clinical and Experimental Immunology Clinical and Experimental Immunology, Wiley, 2012, 167 (1), pp.137-148. ⟨10.1111/j.1365-2249.2011.04488.x⟩ Clinical and Experimental Immunology, 2012, 167 (1), pp.137-148. ⟨10.1111/j.1365-2249.2011.04488.x⟩ Clinical and Experimental Immunology, Wiley, 2012, 167 (1), pp.137-148. 〈10.1111/j.1365-2249.2011.04488.x〉 |
| بيانات النشر: | HAL CCSD, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Male, MESH: Inflammation, MESH: Carcinoma, Transitional Cell/urine, MESH : Aged, MESH : Hepatitis C, Chronic/blood, Immunoenzyme Techniques, MESH: Protein Structure, Tertiary, 0302 clinical medicine, MESH : Culture Media, Conditioned/chemistry, MESH: Chemokine CXCL10/analysis, [ SDV.IMM ] Life Sciences [q-bio]/Immunology, Protein Isoforms, Multiplex, MESH: Protein Isoforms/immunology, MESH: Culture Media, Conditioned/chemistry, Aged, 80 and over, MESH: Recombinant Fusion Proteins/analysis, 0303 health sciences, MESH: Middle Aged, Antibodies, Monoclonal, Atopic dermatitis, MESH: Enzyme-Linked Immunosorbent Assay/methods, MESH : Protein Isoforms/immunology, Neoplasm Proteins, 3. Good health, MESH : Peptide Fragments/immunology, MESH: Urinary Bladder Neoplasms/urine, MESH : Antibodies, Monoclonal/immunology, bladder cancer, MESH : Protein Structure, Tertiary, Agonist, MESH: Chemokine CXCL10/immunology, MESH: Protein Isoforms/analysis, MESH: Hepatitis C, Chronic/blood, Recombinant Fusion Proteins, Immunology, MESH : Body Fluids/chemistry, 03 medical and health sciences, MESH: Peptide Fragments/immunology, MESH : Immunoenzyme Techniques/methods, MESH: Antibodies, Monoclonal/immunology, Humans, MESH : Middle Aged, MESH : Aged, 80 and over, MESH : Protein Processing, Post-Translational, Aged, MESH : Inflammation, MESH: Humans, MESH : Humans, MESH: Adult, MESH: Peptide Fragments/analysis, medicine.disease, Peptide Fragments, Protein Structure, Tertiary, Chemokine CXCL10, Urinary Bladder Neoplasms, chemokines/monokines, Culture Media, Conditioned, MESH: Dipeptidyl Peptidase 4/metabolism, MESH : Recombinant Fusion Proteins/analysis, MESH: Female, Biomarkers, MESH : Protein Isoforms/analysis, hepatitis C virus, MESH : Carcinoma, Transitional Cell/urine, Translational Studies, MESH: Body Fluids/chemistry, medicine.disease_cause, MESH: Neoplasm Proteins/urine, MESH: Aged, 80 and over, Immunology and Allergy, MESH : Female, BCG, MESH : Biomarkers, MESH: Aged, Predictive marker, MESH : Adult, Middle Aged, Body Fluids, MESH : Neoplasm Proteins/urine, MESH : Enzyme-Linked Immunosorbent Assay/methods, MESH : Chemokine CXCL10/immunology, Biomarker (medicine), [SDV.IMM]Life Sciences [q-bio]/Immunology, Female, Adult, medicine.drug_class, MESH : Male, Dipeptidyl Peptidase 4, Hepatitis C virus, Enzyme-Linked Immunosorbent Assay, MESH : Chemokine CXCL10/analysis, MESH : Dipeptidyl Peptidase 4/metabolism, medicine, MESH : Urinary Bladder Neoplasms/urine, MESH : Peptide Fragments/analysis, 030304 developmental biology, Inflammation, Carcinoma, Transitional Cell, business.industry, MESH: Immunoenzyme Techniques/methods, Cancer, Hepatitis C, Chronic, MESH: Male, Transplantation, MESH: Protein Processing, Post-Translational, MESH: Biomarkers, business, Protein Processing, Post-Translational, 030215 immunology |
| الوصف: | Summary The ready access to commercially available multiplex assays and the importance of inflammation in disease pathogenesis has resulted in an abundance of studies aimed at identifying surrogate biomarkers for different clinically important questions. Establishing a link between a biomarker and disease pathogenesis, however, is quite complex, and in some instances this complexity is compounded by post-translational modifications and the use of immunoassays that do not always discriminate between the different forms of the same protein. Herein, we provide a detailed description of an assay system that has been established to discriminate the agonist form of CXCL10 from the NH2-terminal truncated form of the molecule generated by dipeptidylpeptidase IV (DPP4) cleavage. We demonstrate the utility of this assay system for monitoring agonist and antagonist forms of CXCL10 in culture supernatant, patient plasma and urine samples. Given the important role of CXCL10 in chronic inflammatory diseases and its suggested role as a predictive marker in managing patients with chronic hepatitis C, asthma, atopic dermatitis, transplantation, tuberculosis, kidney injury, cancer and other diseases, we believe that our method will be of general interest to the research and medical community. |
| اللغة: | English |
| تدمد: | 0009-9104 1365-2249 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ebab0338538bea4fe091429d1a353338Test https://hal-pasteur.archives-ouvertes.fr/pasteur-01402047Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ebab0338538bea4fe091429d1a353338 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00099104 13652249 |
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