نتائج البحث - "Lactate dehydrogenase"

1

Sources and clinical significance of aspartate aminotransferase increases in COVID-19

المؤلفون: Elena Aloisio, Giulia Colombo, Alberto Dolci, Mauro Panteghini, Claudia Arrigo

المصدر: Clinica Chimica Acta; International Journal of Clinical Chemistry

مصطلحات موضوعية: Clinical Biochemistry, AST, aspartate aminotransferase, Liver injury, Systemic inflammation, Biochemistry, Gastroenterology, law.invention, chemistry.chemical_compound, fluids and secretions, law, polycyclic compounds, Medicine, IL-6, interleukin-6, LDH, lactate dehydrogenase, biology, Liver Diseases, virus diseases, Alanine Transaminase, R2, coefficient of determination, General Medicine, ICU, intensive care unit, Intensive care unit, P-5′-P, pyridoxal-5-phosphate, CRP, C-reactive protein, Alkaline phosphatase, medicine.symptom, medicine.medical_specialty, Aspartate aminotransferase, digestive system, Article, SARS-CoV-2, severe acute respiratory syndrome coronavirus 2 COVID-19. 2019 coronavirus disease, ALT, alanine aminotransferase, Lactate dehydrogenase, Internal medicine, Humans, Clinical significance, IFCC, International Federation of Clinical Chemistry and Laboratory Medicine, Aspartate Aminotransferases, IQR, interquartile range, ALP, alkaline phosphatase, SARS-CoV-2, business.industry, Biochemistry (medical), URL, upper reference limit, COVID-19, medicine.disease, digestive system diseases, OR, odds ratio, CI, confidence interval, HI, hemolysis index, GGT, γ-glutamyltransferase, R, correlation coefficient, Alanine transaminase, chemistry, biology.protein, Creatine kinase, business, CK, creatine kinase

الوصف: Background Aspartate aminotransferase (AST) is often increased in COVID-19 and, in some studies, AST abnormalities were associated with mortality risk. Methods 2054 hospitalized COVID-19 patients were studied. To identify sources of AST release, correlations between AST peak values and other biomarkers of tissue damage, i.e., alanine aminotransferase (ALT) for hepatocellular damage, creatine kinase (CK) for muscle damage, lactate dehydrogenase for multiorgan involvement, alkaline phosphatase and γ-glutamyltransferase for cholestatic injury, and C-reactive protein (CRP) for systemic inflammation, were performed and coefficients of determination estimated. The role of AST to predict death and intensive care unit admission during hospitalization was also evaluated. All measurements were performed using standardized assays. Results AST was increased in 69% of patients. Increases could be fully explained by summing the effects of hepatocellular injury [AST dependence from ALT, 66.8% [95% confidence interval (CI): 64.5–69.1)] and muscle damage [AST dependence from CK, 42.6% (CI: 39.3–45.8)]. We were unable to demonstrate an independent association of AST increases with worse outcomes. Conclusion The mechanisms for abnormal AST in COVID-19 are likely multifactorial and a status related to tissue suffering could play a significant role. The clinical significance of AST elevations remains unclear.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f3d1d325846e5aacf52eb8f0ef551a94Test
https://doi.org/10.1016/j.cca.2021.08.012Test

2

The effect of branched-chain amino acid on muscle damage markers and performance following strenuous exercise: a systematic review and meta-analysis

المؤلفون: Jonathan D. Connor, Daniel Boullosa, Utkarsh Singh, Kenji Doma

المصدر: Applied Physiology, Nutrition, and Metabolism. 46:1303-1313

الوصف: This systematic review and meta-analysis determined whether the ergogenic effects of branched-chain amino acids (BCAA) ameliorated markers of muscle damage and performance following strenuous exercise. In total, 25 studies were included, consisting of 479 participants (age 24.3 ± 8.3 years, height 1.73 ± 0.06 m, body mass 70.8 ± 9.5 kg, females 26.3%). These studies were rated as fair to excellent following the PEDro scale. The outcome measures were compared between the BCAA and placebo conditions at 24 and 48 hours following muscle-damaging exercises, using standardised mean differences and associated p-values via forest plots. Our meta-analysis demonstrated significantly lower levels of indirect muscle damage markers (creatine kinase, lactate dehydrogenase and myoglobin) at 48 hours post-exercise (standardised mean difference [SMD] = −0.41; p < 0.05) for the BCAA than placebo conditions, whilst muscle soreness was significant at 24 hours post-exercise (SMD = −0.28 ≤ d ≤ −0.61; p < 0.05) and 48 hours post-exercise (SMD = −0.41 ≤ d≤ −0.92; p < 0.01). However, no significant differences were identified between the BCAA and placebo conditions for muscle performance at 24 or 48 hours post-exercise (SMD = 0.08 ≤ d ≤ 0.21; p > 0.05). Overall, BCAA reduced the level of muscle damage biomarkers and muscle soreness following muscle-damaging exercises. However, the potential benefits of BCAA for muscle performance recovery is questionable and warrants further investigation to determine the practicality of BCAA for ameliorating muscle damage symptoms in diverse populations. PROSPERO registration number: CRD42020191248. Novelty: BCAA reduces the level of creatine kinase and muscle soreness following strenuous exercise with a dose–response relationship. BCAA does not accelerate recovery for muscle performance.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d86e6ab2bc5d20989971e8d8d425b85aTest
https://doi.org/10.1139/apnm-2021-0110Test

3

The Prognostic Value of Different Levels of Cortisol and High-Sensitivity C-Reactive Protein in Early Acute Pancreatitis

المؤلفون: Amal Hosni, Hanan Sharaf El-Deen Mohammed, Haidi Karam-Allah Ramadan, Entsar H Ahmed, Abeer A Mokhtar, Reham I El-Mahdy

المصدر: The American Journal of the Medical Sciences. 363:435-443

الوصف: Acute pancreatitis (AP) ranges in severity from mild to severe with high mortality. Severe AP, similar to other critical illnesses, is associated with changes in cortisol level. Early increase of high-sensitivity C-reactive protein (hs-CRP) as an inflammatory marker could be an indicator of AP progression. We aimed to assess the level of cortisol and hs-CRP on initial diagnosis of AP and identify their prognostic value.This case-control study included patients with AP and a control group of healthy subjects. Laboratory tests such as liver profile, kidney functions, blood picture, lactate dehydrogenase, blood glucose, and lipogram were evaluated, the severity of AP was determined, the duration of hospitalization, complications, and outcomes were identified, and the serum levels of cortisol and hs-CRP were assessed.There were 90 patients with AP and 60 controls with a higher percent of females in both groups. Serum cortisol and hs-CRP were significantly higher in AP relative to controls and were higher in severe AP relative to mild AP. Significant positive correlation was present between high cortisol and severity of AP (r = 0.520 and p0.001) and negatively with pancreatic necrosis (r= - 0.303 and p = 0.007) and morality (r= - 0.432, p = 0.005) while hs-CRP did not show significant correlation.Different levels of serum cortisol in early AP should be considered on initial diagnosis. High cortisol level was a good prognostic indicator for AP with low mortality. This could have further implications on the appropriate initiation of steroid therapy to prevent necrotizing pancreatitis and lower the mortality. Meanwhile, hs-CRP has a low prognostic value in early AP.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a363f74be4443c8e4fbf93acdf24dc2bTest
https://doi.org/10.1016/j.amjms.2021.09.014Test

4

DETERMINATION OF DIAGNOSTIC ACCURACY OF BIOCHEMICAL PARAMETERS (CRP, LDH & FERRITIN) IN THE DIAGNOSIS OF COVID-19 IN SUSPECTED COVID CASES

المؤلفون: Abdus Sattar, Aamir Ijaz, Tayyaba Ashiq, Sajida Shaheen, Qamar Bashir, Nasir Uddin

المصدر: Pakistan Armed Forces Medical Journal, Vol 71, Iss 5, Pp 1722-1726 (2021)

الوصف: Objective: To determine the diagnostic accuracy of the Lactate Dehydrogenase, C-Reactive Protein and Ferritin in suspected patients of COVID-19. Study Design: Cross-sectional validation study. Place and Duration of Study: Pathology department of Combined Military Hospital Lahore in the month of May 2020. Methodology: We included 101 adult (>18 years) symptomatic suspected COVID-19 patients of both genders. Children, pregnant women and asymptomatic patients were excluded from study. Age, gender and results of Reverse Transcriptase Polymerase Chain Reaction, Lactate Dehydrogenase, C-Reactive Protein, ferritin were recorded. Results: Lactate Dehydrodenase had highest sensitivity (75%) with positive predictive value of 71.6% and diagnostic accuracy of 65.3% among three biochemical parameters studied. Receiver Operator Characteristic curve was studied. Area under curve of Lactate Dehydrogenase (AUC=0.65) and Ferritin (AUC=0.59) reflected their ability to prognosticate the presence of COVID19 disease. However, C-Reactive Protein (AUC=0.42) appeared to be a poor predictor of the disease. Conclusion: Raised serum Lactate Dehydrogenase (>490 U/L) and Ferritin (>152 ng/L) levels can be used to predict the Reverse Transcriptase Polymerase Chain Reaction positivity for COVID-19 in the population of suspected patients of COVID19. However, C-Reactive Protein is a poor predictor of COVID-19.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5f26ec58b2997c09d63730f2b64038bdTest
https://doi.org/10.51253/pafmj.v71i5.5749Test

5

The role of the metabolic status of lymphocytes in the pathogenesis of rheumatoid arthritis

المؤلفون: S. V. Skupnevsky, E. G. Pukhaeva, A. K. Badtiev, F. K. Rurua, F. E. Batagova, Zh. G. Farnieva

المصدر: Современная ревматология, Vol 15, Iss 5, Pp 26-32 (2021)

الوصف: Objective: to study in dynamics metabolic changes in lymphocytes on the model of adjuvant-induced rheumatoid arthritis (RA) in warm-blooded animals.Material and methods. The RA model was elicited by administering Freund's complete adjuvant (AF) to male Wistar rats. Lymphocyte metabolism was corrected with a mixture of citric and succinic acids, which was injected for 4 weeks (at doses of 17 and 88 mg/kg body weight) from the first day of model formation. Animals of the control group were injected subcutaneously with isotonic sodium chloride solution. The total number of leukocytes, the size of lymphocytes and the activity of lactate dehydrogenase (LDH) and succinate dehydrogenase (SDH) in these cells were determined. Changes in the musculoskeletal system were assessed radiographically and actometrically in the «open field» model.Results and discussion. In the first 2 weeks of the experiment in the model group, an increase in the level of leukocytes by 65% compared to the control (p=0.002) was noted, an increase in the activity of lymphocytes SDH by 51% and a decrease in the total («horizontal») mobility of animals by 30% were also revealed. Subsequently, the level of leukocytes decreased by 25%, the activity of LDH and SDH – by 38%, the radius of lymphocytes – by 14% (pConclusion. In animals with adjuvant-induced RA, correction of the metabolic status of lymphocytes with a mixture of citric and succinic acids, which are key substrates of the Krebs cycle, led to a decrease in structural damage of musculoskeletal system and, as a consequence, to the maintenance of normal range of motion.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7d69d83126562fdff5175fb7cd9d160dTest
https://mrj.ima-press.net/mrj/article/view/1193Test

6

Effect of omega‐3 fatty acids supplementation on indirect blood markers of exercise‐induced muscle damage: Systematic review and meta‐analysis of randomized controlled trials

المؤلفون: Gao Xin, Hesam Eshaghi

المصدر: Food Science & Nutrition, Vol 9, Iss 11, Pp 6429-6442 (2021)
Food Science & Nutrition

الوصف: Background Omega 3 fatty acids supplementation may have an attenuative effect on exercise‐induced muscle damage (EIMD) through the cell membrane stabilization. The purpose of the present meta‐analysis was to evaluate the effects of omega 3 fatty acids supplementation, on indirect blood markers of muscle damage following EIMD in trained and untrained individuals. Methods Scopus, Medline, and Google scholar systematically searched up to January 2021. The Cochrane Collaboration tool was used for the quality of studies. Random‐effects model, weighted mean difference (WMD), and 95% confidence interval (CI) were applied for the overall effect estimating. The heterogeneity between studies was evaluated applying the chi‐squared and I2 statistic. Results The outcomes showed a significant effect of omega 3 supplementation on reducing creatine kinase (CK), lactate dehydrogenase (LDH), and myoglobin (Mb) concentrations. In addition, a subgroup analysis indicated a significant reduction in CK, LDH, and Mb concentrations, based on follow‐ups after exercise, studies duration, time of supplementation, and training status. Conclusion The current meta‐analysis indicated an efficacy of omega 3 in reducing CK, LDH, and Mb serum concentration among healthy individuals, overall and in subgroups analysis. Thus, omega 3 should be considered as a priority EIMD recovery agent in interventions.
The current meta‐analysis indicated an efficacy of omega 3 in reducing CK, LDH, and Mb serum concentration among healthy individuals, overall, and in subgroups analysis, based on follow‐ups after exercise, omega 3 doses, studies duration, time of supplementation, exercise type, and training status.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aeaa3bf7b3231c4c6bc5ee50ca3fe74fTest
https://doi.org/10.1002/fsn3.2598Test

7

Prediction of Glioma Stemlike Cell Infiltration in the Non–Contrast-Enhancing Area by Quantitative Measurement of Lactate on Magnetic Resonance Spectroscopy in Glioblastoma

المؤلفون: Shirabe Matsumoto, Seiji Shigekawa, Hideaki Watanabe, Saya Ozaki, Yoshihiro Ohtsuka, Hajime Yano, Akihiro Inoue, Takanori Ohnishi, Riko Kitazawa, Yawara Nakamura, Junya Tanaka, Takeharu Kunieda, Yonehiro Kanemura, Masahiro Nishikawa, Daisuke Yamashita, Satoshi Suehiro

المصدر: World Neurosurgery. 153:e76-e95

مصطلحات موضوعية: Adult, Male, Magnetic Resonance Spectroscopy, Lactate dehydrogenase A, Cell, Carbohydrate metabolism, Creatine, Neurosurgical Procedures, Young Adult, chemistry.chemical_compound, Methionine, Glioma, Temozolomide, Humans, Medicine, Pyruvate Dehydrogenase (Lipoamide), Lactic Acid, RNA, Messenger, education, Antineoplastic Agents, Alkylating, Aged, Aged, 80 and over, education.field_of_study, biology, Brain Neoplasms, business.industry, CD44, Chemoradiotherapy, Adjuvant, Middle Aged, Pyruvate dehydrogenase complex, medicine.disease, Magnetic Resonance Imaging, Mitochondria, nervous system diseases, medicine.anatomical_structure, chemistry, Anaerobic glycolysis, Positron-Emission Tomography, Neoplastic Stem Cells, Cancer research, biology.protein, Female, Surgery, Neurology (clinical), Lactate Dehydrogenase 5, Radiopharmaceuticals, Energy Metabolism, Glioblastoma, business

الوصف: Background We previously reported that glioma stemlike cells (GSCs) exist in the area of the tumor periphery showing no gadolinium enhancement on magnetic resonance imaging. In the present work, we analyzed glucose metabolism to investigate whether lactate could be predictive of tumor invasiveness and of use in detection of the tumor invasion area in glioblastoma multiforme (GBM). Methods The expression of lactate dehydrogenase A (LDH-A) and pyruvate dehydrogenase (PDH) was investigated in 20 patients. In GSC lines, LDH-A and PDH expression also was examined in parallel to assessments of mitochondrial respiration. We then investigated the relationship between lactate/creatine ratios in the tumor periphery measured by magnetic resonance spectroscopy, using learning-compression-model algorithms and phenotypes of GBMs. Results In 20 GBMs, high-invasive GBM expressed LDH-A at significantly higher expression than did low-invasive GBM, whereas low-invasive GBM showed significantly higher expression of PDH than did high-invasive GBM. The highly invasive GSC line showed higher expression of LDH-A and lower expression of PDH compared with low-invasive GSC lines. The highly invasive GSC line also showed the lowest consumption of oxygen and the lowest production of adenosine triphosphate. Lactate levels, as measured by magnetic resonance spectroscopy, showed a significant positive correlation with LDH-A transcript levels, permitting classification of the GBMs into high-invasive and low-invasive phenotypes based on a cutoff value of 0.66 in the lactate/creatine ratio. Conclusions In the tumor periphery area of the highly invasive GBM, aerobic glycolysis was the predominant pathway for glucose metabolism, resulting in the accumulation of lactate. The level of lactate may facilitate prediction of the tumor-infiltrating area on GBM.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::641fa60bd154353f6abba64fe83367fbTest
https://doi.org/10.1016/j.wneu.2021.06.044Test

8

Interleukin‐6 and lactate dehydrogenase expression in a novel ex vivo rocking model of equine corneal epithelial wound healing

المؤلفون: Rachel A. Allbaugh, Anumantha G. Kanthasamy, Gil Ben-Shlomo, Adhithiya Charli, Rita F. Wehrman, Ulrike Genschel

المصدر: Veterinary Ophthalmology. 24:509-519

الوصف: PURPOSE To establish a physiologically relevant ex vivo model of equine corneal epithelial wound healing. METHODS Fourteen equine corneas were randomly assigned to one of two groups: wounded (n = 8) or unwounded (n = 6) controls. In the wounded group, the axial corneal epithelium was removed by applying a 6 mm filter paper disk soaked in 1N-NaOH for 60 s. Corneas were subsequently cultured using an air-liquid interface model. Evaluation of corneal healing was performed daily, and culture medium was collected. Corneas were randomly assigned to undergo processing via histopathology and RNAscope in situ hybridization for interleukin-6 (IL-6) and alpha-smooth muscle actin (αSMA) expression at T24, T48, and T72 h after wounding. Media of the cultured corneas were evaluated for the presence of lactate dehydrogenase (LDH) by a colorimetric assay. RESULTS The ulcerated area of the wounded corneas decreased over time and all corneas healed within 72 h. Histologically, normal corneal architecture was observed including healthy epithelium (in areas other than the ulcerated ones), minimal stromal edema, intact endothelium, and Descemet's membrane. IL-6 expression was increased in wounded corneas compared with unwounded controls. LDH expression was elevated for both wounded and unwounded corneas at T24 but decreased substantially and was not detected at T48 in media from wounded and unwounded corneas, respectively. No αSMA expression was detected from either wounded or unwounded corneas. CONCLUSIONS The equine air-liquid interface, ex vivo, corneal epithelial wound healing model is effective and physiologically relevant. This model can be used in future studies evaluating various corneal therapies.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ea192aadfdfee5204f426e8a84a2438eTest
https://doi.org/10.1111/vop.12935Test

9

Protective Effect of Topiroxostat on Myocardial Injury Induced by Lipopolysaccharide

المؤلفون: Jiong Liu, Yongguang Lao, Yueming Chen, Chunsheng Li, Xiangdong Zhang, Deli Zhan, Weihua Ding

المصدر: Journal of Surgical Research. 271:171-179

مصطلحات موضوعية: Lipopolysaccharides, Lipopolysaccharide, Pyridines, Apoptosis, Inflammation, medicine.disease_cause, Andrology, chemistry.chemical_compound, Lactate dehydrogenase, Nitriles, Animals, Medicine, TUNEL assay, biology, business.industry, Malondialdehyde, Rats, Oxidative Stress, chemistry, biology.protein, Surgery, Creatine kinase, medicine.symptom, business, Oxidative stress

الوصف: Background Myocardial injury induced by sepsis is the most common cause of death. Topiroxostat has been found to have organ protective effects, but its role in septic shock-related cardiomyocyte damage is still unclear and needs further study. Material and methods An endotoxemic shock model in rats was constructed. After topiroxostat treatment, hemodynamic parameters, myocardial injury marker enzymes, oxidative stress, myocardial injury, and apoptosis were measured by polyphysiograph, enzyme-linked immunosorbent assay, hematoxylin and eosin staining, TUNEL staining, and western blot. During in vitro experiments, the effect of topiroxostat on cell vitality, oxidative stress, inflammatory factors, apoptosis-related markers, phosphorylated-p65 (p-p65) and p65 expressions were measured by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), flow cytometry, enzyme-linked immunosorbent assay, quantitative real-time polymerase chain reaction, and western blot. Results Topiroxostat improved myocardial dysfunction and superoxide dismutase activity while suppressing levels of creatine kinase, lactate dehydrogenase and malondialdehyde in serum of endotoxemic shock rats. Additionally, topiroxostat augmented dry-wet weight ratios of the hearts in rats. Meanwhile, topiroxostat was proved to alleviate interstitial edema and apoptosis in myocardial tissues of endotoxemic shock rats. During in vitro experiments, topiroxostat pretreatment elevated lipopolysaccharide (LPS)-induced H9c2 cell vitality, and alleviated oxidative stress and inflammation. Moreover, topiroxostat pretreatment downregulated apoptosis-related markers, p-p65, and p-p65/p65 levels in LPS-induced H9c2 cells. Conclusions Topiroxostat attenuated LPS-induced myocardial injury via repressing apoptosis and oxidative stress.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::837efff2d6ee4ef212cc00ba11517c49Test
https://doi.org/10.1016/j.jss.2021.08.047Test

10

Diagnostic and early prognostic value of serum CRP and LDH levels in patients with possible COVID-19 at the first admission

المؤلفون: Mustafa Güzel, Dervis Tosun, Doğan Akdoğan, Orhan Akpınar

المصدر: Journal of infection in developing countries, 15(6):766-772

الوصف: Introduction: COVID-19 is the infection caused by the new coronavirus. Specific treatment for COVID-19 has not been established, yet. It is important to determine the disease severity of the patients at the first admission. Therefore, the exploration of biomarkers is deemed necessary. We aimed to assess the diagnostic and early prognostic value of CRP and LDH levels in possible COVID-19 patients presenting with a severe clinical picture. Methodology: We evaluated the correlations of relevant routine laboratory test results with disease severity in COVID-19 patients admitted to our infectious diseases clinic. Patients were divided into severe and non-severe disease groups based on clinical findings, oxygen saturation levels in the arterial blood, biochemical test results, and radiological findings. Differences in the findings between the two disease severity groups were examined to determine potential biomarkers. Results: Median age and the CRP and LDH levels in the severe disease group were statistically significantly higher compared to the nonsevere group (p < 0.0001). No other parameters statistically significant differences have been observed between the two groups (P > 0.05). Conclusions: CRP and LDH levels were positively correlated with lung lesions in early-stage COVID-19, potentially reflecting disease severity. Because LDH and CRP levels can potentially reflect the pulmonary function, they can be potential predictors of COVID-19- related respiratory failure. For avoiding poor prognosis; LDH and CRP should be considered as potential predictors for identifying the need for thoracic CT scans, close monitoring of pulmonary function, and aggressive supportive therapy early in the course of COVID-19.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3d7c44b5298af9a8f2d5f6b53d48c932Test
https://doi.org/10.3855/jidc.14072Test

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