Synthesis of Adenine-based Fluorescent and Naked-eye Chemosensors: Specific DNA Probes for The Detection of Bacterial Pathogens
| العنوان: | Synthesis of Adenine-based Fluorescent and Naked-eye Chemosensors: Specific DNA Probes for The Detection of Bacterial Pathogens |
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| المؤلفون: | Said Nadeem, Ömür Baysal, Ragıp Soner Silme, Burak Şener |
| المساهمون: | MÜ, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, Şener, Burak, Baysal, Ömür, Silme, Ragıp Soner |
| المصدر: | Acta Chimica Slovenica, Vol 68, Iss 4, Pp 781-790 (2021) |
| بيانات النشر: | Slovenian Chemical Society, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | DNA, Bacterial, Alkylation, viruses, genetic processes, detection, DNA, Single-Stranded, kit designing, Biosensing Techniques, Bacillus subtilis, environment and public health, chemistry.chemical_compound, bacterial pathogens, dna probe, QD1-999, Fluorescent Dyes, General Environmental Science, biology, nanotechnology, Adenine, Hybridization probe, biology.organism_classification, Fluorescence, Thymine, enzymes and coenzymes (carbohydrates), Chemistry, DNA probe, chemistry, Biochemistry, naked-eye chemosensor, health occupations, General Earth and Planetary Sciences, Colorimetry, Bacterial pathogens, Naked eye, DNA Probes, Clavibacter michiganensis, Nitrocellulose, DNA |
| الوصف: | A rapid and confident tool to identify and diagnose bacterial pathogens with more accuracy using DNA as fingerprints is necessary. Herein, we report a smart chemosensor having a terminal adenine sticking to the thymine of single-stranded DNA (ssDNA) through supramolecular interactions and, which leaves ssDNA when the same ssDNA matches with the targeting desired DNA. We have synthesized a naked-eye coloured chemosensor with carbazole. As a model genetic material, DNA of Clavibacter michiganensis subsp. michiganensis was hybridized to ssDNA and immobilized over nitrocellulose membrane. The prepared adenine-chemosensor, by passing through the nitrocellulose-ssDNA membrane caused the formation of ssDNA nitrocellulose-ssDNA-adenine-chemosensor. FTIR results of the immobilized ssDNAs showed that the matching of same ssDNA releases the adenine-chemosensor from the surface of nitrocellulose-ssDNA that results in formation of the double stranded DNA. The selectivity of chemosensor was also confirmed with different bacterial DNA (Bacillus subtilis) as control. These data highlights accurate and reliable results of a new diagnostic kit prototype promising for further studies, which is able to diagnose DNA quickly and precisely. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1580-3155 1318-0207 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4b82f00ce1b9ee8d5520684baf344d24Test https://journals.matheo.si/index.php/ACSi/article/view/6749Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4b82f00ce1b9ee8d5520684baf344d24 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15803155 13180207 |
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