The dynamic shuttling of SIRT1 between cytoplasm and nuclei in bronchial epithelial cells by single and repeated cigarette smoke exposure
| العنوان: | The dynamic shuttling of SIRT1 between cytoplasm and nuclei in bronchial epithelial cells by single and repeated cigarette smoke exposure |
|---|---|
| المؤلفون: | Yanagisawa, S, Baker, JR, Vuppusetty, C, Koga, T, Colley, T, Fenwick, P, Donnelly, LE, Barnes, PJ, Ito, K, Yildirim, AÖ |
| المساهمون: | Yildirim, AÖ |
| المصدر: | PLoS ONE PLoS ONE, Vol 13, Iss 3, p e0193921 (2018) |
| بيانات النشر: | Public Library of Science (PLoS), 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Cytoplasm, Pulmonology, lcsh:Medicine, medicine.disease_cause, Biochemistry, Epithelium, Antioxidants, chemistry.chemical_compound, Habits, Sirtuin 1, Animal Cells, Medicine and Health Sciences, Smoking Habits, Public and Occupational Health, lcsh:Science, Multidisciplinary, biology, Chemistry, Reverse Transcriptase Polymerase Chain Reaction, Smoking, 3. Good health, Cell biology, Enzymes, Dismutases, Cellular Types, Anatomy, Cellular Structures and Organelles, Research Article, Cell type, Substance-Related Disorders, General Science & Technology, Chronic Obstructive Pulmonary Disease, Blotting, Western, SOD2, Bronchi, Superoxide dismutase, 03 medical and health sciences, Mental Health and Psychiatry, MD Multidisciplinary, medicine, Humans, Phosphatidylinositol, PI3K/AKT/mTOR pathway, Cell Nucleus, Messenger RNA, Behavior, Dose-Response Relationship, Drug, Superoxide Dismutase, lcsh:R, Biology and Life Sciences, Proteins, Smoking Related Disorders, Epithelial Cells, Cell Biology, Oxidative Stress, 030104 developmental biology, Biological Tissue, biology.protein, Enzymology, lcsh:Q, Oxidative stress |
| الوصف: | SIRT1 (silent information regulator 2 homolog 1) is a crucial cellular survival protein especially in oxidative stress environments, and has been thought to locate within the nuclei, but also known to shuttle between cytoplasm and nuclei in some cell types. Here, we show for the first time the dynamics of SIRT1 in the presence of single or concurrent cigarette smoke extract (CSE) exposure in human bronchial epithelial cells (HBEC). In BEAS-2B HBEC or primary HBEC, SIRT1 was localized predominantly in cytoplasm, and the CSE (3%) induced nuclear translocation of SIRT1 from cytoplasm in the presence of L-buthionine sulfoximine (an irreversible inhibitor of γ-glutamylcystein synthetase), mainly through the activation of phosphatidylinositol 3-kinase (PI3K) α subunit. This SIRT1 nuclear shuttling was associated with FOXO3a nuclear translocation and the strong induction of several anti-oxidant genes including superoxide dismutase (SOD) 2 and 3; therefore seemed to be an adaptive response. When BEAS-2B cells were pretreated with repeated exposure to a lower concentration of CSE (0.3%), the CSE-induced SIRT1 shuttling and resultant SOD2/3 mRNA induction were significantly impaired. Thus, this result offers a useful cell model to mimic the impaired anti-oxidant capacity in cigarette smoking-associated lung disease such as chronic obstructive pulmonary disease. |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6277f767bab6f62e6905251132153012Test http://hdl.handle.net/10044/1/58025Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6277f767bab6f62e6905251132153012 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |