Association between Exosomal miRNAs and Coronary Artery Disease by Next-Generation Sequencing
| العنوان: | Association between Exosomal miRNAs and Coronary Artery Disease by Next-Generation Sequencing |
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| المؤلفون: | Sheng-Nan Chang, Jien-Jiun Chen, Jo-Hsuan Wu, Yao-Te Chung, Jin-Wun Chen, Chu-Hsuan Chiu, Chia-Ju Liu, Meng-Tsun Liu, Yi-Cheng Chang, Chin Li, Jou-Wei Lin, Juey-Jen Hwang, Wen-Pin Lien |
| المصدر: | Cells; Volume 11; Issue 1; Pages: 98 Cells Cells, Vol 11, Iss 98, p 98 (2022) |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Male, QH301-705.5, High-Throughput Nucleotide Sequencing, General Medicine, Coronary Artery Disease, exosomes, Middle Aged, Article, MicroRNAs, micro RNAs, Linear Models, Humans, Female, next-generation sequencing, cardiovascular diseases, Biology (General), coronary artery disease, Aged |
| الوصف: | Background: Among various bio-informative molecules transferred by exosomes between cells, micro RNAs (miRNAs), which remain remarkably stable even after freeze-and-thaw cycles, are excellent candidates for potential biomarkers for coronary artery disease (CAD). Methods: Blood samples were collected from the coronary arteries of 214 patients diagnosed with three-vessel CAD and 140 without CAD. After precipitation extraction, the amounts of exosomes were found to decrease with increased age and three-vessel CAD. Next-generation sequencing was performed to further explore the possible relationship between exosomal miRNAs and CAD. Results: Eight exosomal miRNAs showed altered expression associated with CAD. The up-regulated miRNAs in CAD were miRNA-382-3p, miRNA-432-5p, miRNA-200a-3p, and miRNA-3613-3p. The down-regulated miRNAs were miRNA-125a-5p, miRNA-185-5p, miRNA-151a-3p, and miRNA-328-3p. Conclusion: We successfully demonstrated particular exosomal miRNAs that may serve as future biomarkers for CAD. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2073-4409 |
| DOI: | 10.3390/cells11010098 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29f88d2b398e011fd00fda5aed56289aTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....29f88d2b398e011fd00fda5aed56289a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20734409 |
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| DOI: | 10.3390/cells11010098 |