دورية أكاديمية
Ultrastructural Analysis of Prune Dwarf Virus Intercellular Transport and Pathogenesis
| العنوان: | Ultrastructural Analysis of Prune Dwarf Virus Intercellular Transport and Pathogenesis |
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| المؤلفون: | Edmund Kozieł, Katarzyna Otulak-Kozieł, Józef J. Bujarski |
| المصدر: | International Journal of Molecular Sciences, Vol 19, Iss 9, p 2570 (2018) |
| بيانات النشر: | MDPI AG |
| سنة النشر: | 2018 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | viral intercellular transport, immunolocalization, plasmodesmata, 3D protein structure, Prune dwarf virus, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Prune dwarf virus (PDV) is an important viral pathogen of plum, sweet cherry, peach, and many herbaceous test plants. Although PDV has been intensively investigated, mainly in the context of phylogenetic relationship of its genes and proteins, many gaps exist in our knowledge about the mechanism of intercellular transport of this virus. The aim of this work was to investigate alterations in cellular organelles and the cell-to-cell transport of PDV in Cucumis sativus cv. Polan at ultrastructural level. To analyze the role of viral proteins in local transport, double-immunogold assays were applied to localize PDV coat protein (CP) and movement protein (MP). We observe structural changes in chloroplasts, mitochondria, and cellular membranes. We prove that PDV is transported as viral particles via MP-generated tubular structures through plasmodesmata. Moreover, the computer-run 3D modeling reveals structural resemblances between MPs of PDV and of Alfalfa mosaic virus (AMV), implying similarities of transport mechanisms for both viruses. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1422-0067 |
| العلاقة: | http://www.mdpi.com/1422-0067/19/9/2570Test; https://doaj.org/toc/1422-0067Test; https://doaj.org/article/8a196a28c9104c91a6d41b9c77bb02b1Test |
| DOI: | 10.3390/ijms19092570 |
| الإتاحة: | https://doi.org/10.3390/ijms19092570Test https://doaj.org/article/8a196a28c9104c91a6d41b9c77bb02b1Test |
| رقم الانضمام: | edsbas.B23765D4 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 14220067 |
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| DOI: | 10.3390/ijms19092570 |