Use of NRP1, a novel biomarker, along with VEGF-C, VEGFR-3, CCR7 and SEMA3E, to predict lymph node metastasis in squamous cell carcinoma of the tongue
| العنوان: | Use of NRP1, a novel biomarker, along with VEGF-C, VEGFR-3, CCR7 and SEMA3E, to predict lymph node metastasis in squamous cell carcinoma of the tongue |
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| المؤلفون: | Sanam Bakhshishayan, Yosuke Shogen, Atsushi-Doksa Lee, Noriaki Tanaka, Mikihiko Kogo, Hani Al-Shareef, Shin-ichiro Hiraoka |
| المصدر: | Oncology Reports |
| بيانات النشر: | Spandidos Publications, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | squamous cell carcinoma, Male, 0301 basic medicine, Cancer Research, Pathology, vascular endothelial growth factor-D, Vascular Endothelial Growth Factor C, C-C chemokine receptor type 7, Semaphorins, Metastasis, 0302 clinical medicine, Neuropilin 1, vascular endothelial growth factor-C, Aged, 80 and over, Mouth neoplasm, semaphorin 3E, Articles, General Medicine, Middle Aged, Prognosis, Gene Expression Regulation, Neoplastic, medicine.anatomical_structure, Oncology, Vascular endothelial growth factor C, Lymphatic Metastasis, 030220 oncology & carcinogenesis, Carcinoma, Squamous Cell, Female, Mouth Neoplasms, Adult, Receptors, CCR7, medicine.medical_specialty, Biology, Disease-Free Survival, 03 medical and health sciences, Biomarkers, Tumor, Lymphatic vessel, medicine, Carcinoma, Humans, Aged, Lymphatic Vessels, Oncogene, lymphatic vessel density, vascular endothelial growth factor receptor-3, medicine.disease, Neuropilin-1, 030104 developmental biology, Cancer research, Lymph Nodes |
| الوصف: | Lymph node (LN) metastasis has been suggested as a major prognostic factor for oral cancer. Knockdown of the growth factors and receptors involved in these metastatic mechanisms could significantly reduce LN metastasis and improve the survival of oral cancer patients after treatment. The present study, therefore, aimed to evaluate the expression levels of the following growth factors and receptors in squamous cell carcinoma (SCC) of the tongue: the vascular endothelial growth factor (VEGF)‑C and VEGF‑D, which bind to the cell surface tyrosine kinase receptor VEGF receptor‑3 (VEGFR‑3); C‑C motif chemokine receptor 7 (CCR7); neuropilin (NRP)1 and NRP2; and semaphorin 3E (SEMA3E). Furthermore, we assessed microvessel density (MVD) and lymphatic vessel density (LVD) to demonstrate the correlation between these factors and regional LN metastasis, with respect to the clinicopathological features. Finally, we analyzed the correlation between these proteins and overall or disease‑free survival, in order to demonstrate their prognostic value. Univariate analysis revealed a significant association between LN metastasis and the expression levels of VEGF‑C, VEGFR‑3, CCR7, NRP1, and SEMA3E, as well as LVD, in SCC cells. In contrast, multivariate analysis identified associations between LN metastasis and NRP1 expression, as well as between LN metastasis and LVD; however, no correlation was found between LN metastasis and the expression levels of the other proteins. The expression levels of VEGF‑C, VEGFR‑3, NRP1, and SEMA3E, as well as LVD, were correlated with disease‑free survival time. These results indicate that LN metastasis is associated with poor survival in SCC. This study suggests that NRP1 expression and LVD are independent factors that are likely to predict the risk of LN metastasis in SCC of the tongue, whereas the expression of VEGF‑C, VEGFR‑3, CCR7, and SEMA3E are non‑independent predictive factors. |
| تدمد: | 1791-2431 1021-335X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e8b7bb62856c03042a45ecb3279529f8Test https://doi.org/10.3892/or.2016.5116Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e8b7bb62856c03042a45ecb3279529f8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17912431 1021335X |
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