Whole-Genome Sequencing for Routine Pathogen Surveillance in Public Health: a Population Snapshot of Invasive Staphylococcus aureus in Europe
| العنوان: | Whole-Genome Sequencing for Routine Pathogen Surveillance in Public Health: a Population Snapshot of Invasive Staphylococcus aureus in Europe |
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| المؤلفون: | David M. Aanensen, Edward J. Feil, Matthew T. G. Holden, Janina Dordel, Corin A. Yeats, Artemij Fedosejev, Richard Goater, Santiago Castillo-Ramírez, Jukka Corander, Caroline Colijn, Monika A. Chlebowicz, Leo Schouls, Max Heck, Gerlinde Pluister, Raymond Ruimy, Gunnar Kahlmeter, Jenny íÅhman, Erika Matuschek, Alexander W. Friedrich, Julian Parkhill, Stephen D. Bentley, Brian G. Spratt, Hajo Grundmann, Helmut Mittermayer, Karina Krziwanek, Sabine Stumvoll, Walter Koller, Olivier Denis, Marc Struelens, Dimitr Nashev, Ana Budimir, Smilja Kalenic, Despo Pieridou-Bagatzouni, Vladislav Jakubu, Helena Zemlickova, Henrik Westh, Anders Rhod Larsen, Robert Skov, Frederic Laurent, Jerome Ettienne, Birgit Strommenger, Wolfgang Witte, Sofia Vourli, Alkis Vatopoulos, Anni Vainio, Jaana Vuopio-Varkila, Miklos Fuzi, Erika UngvíÅóóóári, Stephan Murchan, Angela Rossney, Edvins Miklasevics, Arta Balode, Gunnsteinn Haraldsson, Karl G. Kristinsson, Monica Monaco, Annalisa Pantosti, Michael Borg, Marga van Santen-Verheuvel, Xander Huijsdens, Lillian Marstein, Trond Jacobsen, Gunnar Skov Simonsen, Marta Airesde-Sousa, Herminia de Lencastre, Agnieszka Luczak-Kadlubowska, Waleria Hryniewicz, Monica Straut, Irina Codita, Maria Perez-Vazquez, Jesus Oteo Iglesias, Vesna Cvitkovic Spik, Manica Mueller-Premru, Sara Haeggman, Barbro Olsson-Liljequist, Matthew Ellington, Angela Kearns |
| المساهمون: | Wellcome Trust, Medical Research Council (Reino Unido), University of St Andrews. School of Medicine, University of St Andrews. Infection Group, University of St Andrews. Biomedical Sciences Research Complex, Microbes in Health and Disease (MHD), National Institute for Health Research, Medical Research Council (MRC) |
| المصدر: | mBio, Vol 7, Iss 3 (2016) MBio, 7 (3 mBio 2016, ' Whole-genome sequencing for routine pathogen surveillance in Public Health : a population snapshot of invasive staphylococcus aureus in Europe ', mBio, vol. 7, no. 3, e00444-16 . https://doi.org/10.1128/mBio.00444-16Test mBio, Vol 7, Iss 3, p e00444-16 (2016) Mbio, 7(3):ARTN e00444-16. AMER SOC MICROBIOLOGY |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, METHICILLIN-RESISTANT, Population level, MRSA, Disease Outbreaks, RA0421, RA0421 Public health. Hygiene. Preventive Medicine, education.field_of_study, SINGLE-NUCLEOTIDE POLYMORPHISMS, High-Throughput Nucleotide Sequencing, Staphylococcal Infections, QR1-502, 3. Good health, COMMUNITY, Europe, Epidemiological Monitoring, Snapshot (computer storage), epidemiology, BDC, Research Article, STRAIN, Staphylococcus aureus, medicine.medical_specialty, GENES, 030106 microbiology, Population, NDAS, Context (language use), Biology, Microbiology, 03 medical and health sciences, Antibiotic resistance, SDG 3 - Good Health and Well-being, Virology, CLONE, Drug Resistance, Bacterial, medicine, Humans, Computer Simulation, Road map, education, Whole genome sequencing, business.industry, Public health, microbiology, Computational Biology, Sequence Analysis, DNA, Data science, EVOLUTION, Ingénierie biomédicale, Biotechnology, VIRULENCE, The spread of antibiotic-resistant bacteria is a public health emergency of global concern, threatening medical intervention at every level of health care delivery, Microbiologie et protistologie [bacteriol.virolog.mycolog.], business, Genome, Bacterial, Software, European SRL Working Group, TOXIC-SHOCK-SYNDROME |
| الوصف: | The implementation of routine whole-genome sequencing (WGS) promises to transform our ability to monitor the emergence and spread of bacterial pathogens. Here we combined WGS data from 308 invasive Staphylococcus aureus isolates corresponding to a pan-European population snapshot, with epidemiological and resistance data. Geospatial visualization of the data is made possible by a generic software tool designed for public health purposes that is available at the project URL (http:// www.microreact.org/project/EkUvg9uY?tt=rc). Our analysis demonstrates that high-risk clones can be identified on the basis of population level properties such as clonal relatedness, abundance, and spatial structuring and by inferring virulence and resistance properties on the basis of gene content. We also show that in silico predictions of antibiotic resistance profiles are at least as reliable as phenotypic testing. We argue that this work provides a comprehensive road map illustrating the three vital components for future molecular epidemiological surveillance: (i) large-scale structured surveys, (ii) WGS, and (iii) communityoriented database infrastructure and analysis tools. IMPORTANCE The spread of antibiotic-resistant bacteria is a public health emergency of global concern, threatening medical intervention at every level of health care delivery. Several recent studies have demonstrated the promise of routine wholegenome sequencing (WGS) of bacterial pathogens for epidemiological surveillance, outbreak detection, and infection control. However, as this technology becomes more widely adopted, the key challenges of generating representative national and international data sets and the development of bioinformatic tools to manage and interpret the data become increasingly pertinent. This study provides a road map for the integration of WGS data into routine pathogen surveillance. We emphasize the importance of large-scale routine surveys to provide the population context for more targeted or localized investigation and the development of open-access bioinformatic tools to provide the means to combine and compare independently generated data with publicly available data sets. 0 SCOPUS: ar.j info:eu-repo/semantics/published |
| وصف الملف: | 1 full-text file(s): application/pdf; application/pdf |
| اللغة: | English |
| تدمد: | 2150-7511 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4b49265659ca3057d5f72ad31631d5b5Test https://www.bib.irb.hr/990521Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4b49265659ca3057d5f72ad31631d5b5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 21507511 |
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