المؤلفون: Chi Jingjing, Zhang Li, Ren Weike, Wang Lili, Tian Xiangxue, Li Xiuli, Yan Minghua, Jiang Shan, Dong Zhimin, Lu Chao, Cheng Li, Li Fuqiang

المصدر: Viral Immunology. 34:714-721

مصطلحات موضوعية: China, medicine.medical_specialty, Veterinary medicine, Farms, Swine, animal diseases, Immunology, Pseudorabies, Virus, Seroepidemiologic Studies, Virology, Epidemiology, medicine, Animals, Seroprevalence, Swine Diseases, Univariate analysis, biology, biology.organism_classification, Serum samples, Herpesvirus 1, Suid, Increased risk, biology.protein, Molecular Medicine, Female, Antibody

الوصف: Pseudorabies (PR), the causative agent of Aujeszky's disease, has rapidly increased in recent years and has caused significant economic losses. To understand the seroprevalence and epidemiological characteristics of PR in Tianjin, China, a total of 23,627 blood and 1,093 tissue samples were collected from 228 pig farms during January 2010 to December 2018. The Pseudorabies virus (PRV) glycoprotein E (gE) antibody was tested by enzyme-linked immunosorbent assay (ELISA), and wild-type PRV (WT PRV) was detected by gE-polymerase chain reaction (PCR). Macroscopic and microscopic lesions were observed in tissue samples. The results showed that 46.70% of the serum samples and 49.76% of pig farms were seropositive for PRV gE antibody based on the ELISA results, and 13.54% of the tissue samples were positive for WT PRV detected by PCR. The positive rate of serum samples increased rapidly after 2011 and reached 62.40% in 2013. Although it gradually decreased from 2014 to 2018, the positive rate of serum samples remained at a high level. The positive rate of pig farms showed the same trend. Moreover, after 2011, the detection rate of WT PRV was increased rapidly and was significantly higher than in 2010 and 2011. Macroscopic and microscopic lesions were observed in various tissues during histopathological examination. Based on univariate analysis, the increased risk of seropositivity was associated with the immune status and infection in sows and fattening pigs. These findings demonstrate that PR was prevalent in the region of Tianjin, China. These epidemiological data can assist in the control of PR.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::029b7a2b5c053454f5b17f69255661e1Test
https://doi.org/10.1089/vim.2021.0069Test

المؤلفون: Wang Lili, Cheng Li, Yan Minghua, Ren Weike, Zheng Li, Zhang Li, Jiang Shan, Lu Chao, Li Xiuli, Tian Xiangxue, Li Fuqiang

المصدر: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases. 96

مصطلحات موضوعية: Microbiology (medical), Serotype, China, animal structures, Fowl, Adenoviridae Infections, Chick Embryo, Serogroup, Microbiology, Virus, Inclusion bodies, Tissue culture, Genetics, Animals, Molecular Biology, Ecology, Evolution, Behavior and Systematics, Feces, Phylogeny, Poultry Diseases, Specific-pathogen-free, biology, Inoculation, Aviadenovirus, biology.organism_classification, Infectious Diseases, Chickens

الوصف: A fowl aviadenovirus serotype 4 (FAdV-4), Y17215-1, was isolated from the liver of chickens with Hydropericardium-hepatitissyndrome(HHS) in a chicken farm of Tianjin, China. Obvious cytopathic effects were observed in the infected chicken liver hepatocellular carcinoma cell line (LMH cells) at 24 h post infection (hpi), which consisted of enlarger and rounder shape of cells. The typical and specific green fluorescence was observed by indirect immunofluorescence assay (IFA). Tissue Culture Infectious Dose50 (TCID50) of it measured after five stable passage in LMH cells reached 106.5TCID50/0.1 ml. The strain was inoculated through allantoic membrane of 10-day specific pathogen free(SPF) Chick embryos, the thicker allantoic membranes were observed at 120 hpi. 7-day-old SPF chickens were inoculated with the strain via intramuscular (i.m.) or intranasal (i.n.) injection which resulted in 100% mortality of test chickens. Additionally, the sickness and death of cohabitation chickens in the test group were observed which indicated that the virus can infect healthy chickens by horizontal transmission. The sick chickens showed depression, anorexia and diarrhea with green watery feces. Y17215-1-inoculated chickens mainly presented swollen liver with blood spot, and the enhancement of effusion or yellow gel like effusion that were observed in the pericardium through necropsy. Histopathological examination showed focal necrosis of hepatocytes and characteristic eosinophilic inclusion bodies in the cytoplasm. The results showed that the Y17215-1 isolate had high pathogenicity to SPF chickens. The phylogenetic analysis of the major structural proteins including hexon, fiber-1 and fiber-2 revealed that Y17215-1 strain belongs to C species of fowl aviadenovirus of aviadenovirus family, and has high homology with other Chinese strains isolated in recent years, but was distinct from ON1、MX-SHP95、KR5 and other foreign isolates. This study laid a foundation for further study of epidemiological investigation, pathogenic mechanism as well as the diagnosis and control technology of FAdV-4.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::593739d74ad01623d07c9a048b9e9076Test
https://pubmed.ncbi.nlm.nih.gov/34508884Test

المؤلفون: Li Xuesi, Li Fuqiang, Li Shaoliang, Ruijie Tian, Xiaolong Hu, Kangli Wang, Cao Zhenhua, Peixin He, Yan Peixun

المصدر: Food research international (Ottawa, Ont.). 148

مصطلحات موضوعية: China, biology, Chemistry, Microbiota, food and beverages, Bacillus, Methanosarcina, biology.organism_classification, Methanosaeta, Flavoring Agents, Clostridium, Lactobacillus, Taste, Fermentation, Pediococcus, Food science, Flavor, Food Science

الوصف: Fermented grain (FG), a complex and unique ecosystem, is the main microbial habitats, biochemical reaction system and direct source of flavor compounds for the Chinese strong-flavor Baijiu (CSFB) production. However, the dynamics of physicochemical properties, prokaryotic community and flavor compounds of FGs during the long-term fermentation process are still not completely clear. Here, the above topics on FGs in the actual production process were comprehensively studied by using a combination of physicochemical analysis, GC-MS detection and Illumina HiSeq sequencing methods. The whole fermentation process could be divided into two stages including early (0-25d) and the later stage (25-60d) based on the dynamics of FG physicochemical properties and the changes of prokaryotic community diversity. A total of 41phyla and 364 genera were detected, and 9 of them were dominant genera in FG complex ecosystem, including Lactobacillus, Pediococcus, Ochrobactrum, Bacillus etc. Among them, the dynamics of 29 top10 genera in FGs were mainly influenced by the starch and total acid, followed by NH4+ and ethanol, and 7 genera (hubs, e.g., Clostridium, Methanosaeta, Bacillus, etc.) of them may play important roles in FG ecosystem stability. A total of 71 volatiles including 33 esters, 14 alcohols, 9 fatty acids, 5 phenols, and 10 other compounds were detected in the FGs, and most of them formed in the early stage. Some important flavor substances (e.g., ethyl octanoate, 3-methylbutanol, hexanoate, etc.) increased in the later stage. Moreover, the formation of some flavor compound might require multiple microbes involved. For instance, ten of the top10 genera, including Lactobacillus, Clostridium, Methanosarcina, Sedimentibacter, Bacillus, etc., were significantly and positively correlated with four important esters. This study may help to clarify the complex correlations among prokaryotic community, physicochemical properties and flavors, allow the improvement of CSFB quality by using bioaugmentation and/or controlling environmental factors, and shed more light on the ecological rules guiding community assembly in FGs.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dad19069e12f9e4473c84feead74b63dTest
https://pubmed.ncbi.nlm.nih.gov/34507770Test

المؤلفون: Li Xuan, Lintao Liu, Xietian Chen, Li Fuqiang, Wang Yucai, Hengjia Zhang, Deng Haoliang

المصدر: Scientific Reports
Scientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)

مصطلحات موضوعية: Stomatal conductance, China, Agricultural Irrigation, Starch, Science, Deficit irrigation, Plastic film, Drip irrigation, Article, chemistry.chemical_compound, Soil, Water-use efficiency, Photosynthesis, Transpiration, Solanum tuberosum, Multidisciplinary, Conservation of Water Resources, biology, fungi, food and beverages, Water, Agriculture, biology.organism_classification, Plant Leaves, Horticulture, Plant Tubers, chemistry, Seedling, Plant stress responses, Medicine, Plant sciences

الوصف: The effects of the amount and timing of regulated deficit drip irrigation under plastic film on potato (‘Qingshu 168’) growth, photosynthesis, yield, water use efficiency, and quality were examined from 2017 to 2019 in cold and arid northwestern China. A two-factor randomized design was used with two levels of regulated deficit irrigation (mild, moderate) applied in four stages of potato growth (seedling, tuber initiation, tuber bulking, starch accumulation). Growth and photosynthetic parameters were determined in each growth stage, and yield and quality were measured after harvest. The net photosynthetic rate, stomatal conductance, and transpiration rate decreased significantly under water deficit in the tuber formation and starch accumulation stages, and the decreases were greater with moderate than mild water deficit. Although water deficit reduced potato yields, potato subjected to mild water deficit in the seedling stage had the highest yield at 43,961.91 kg·ha−1, followed by those subjected to mild water deficit in the starch accumulation stage at 42,262.25 kg·ha−1, which were decreases of only 4.50% and 8.19%, respectively, compared with potato under full irrigation. The highest water use efficiency was in potatoes subjected to mild water deficit in the seedling stage (8.67 kg·m−3), with the second highest in those subjected to moderate water deficit in the seedling stage (8.28 kg·m−3), which were significant improvements by 10.87% and 5.84%, respectively, compared with full irrigation. The highest overall quality was in potatoes subjected to mild and moderate water deficit in the seedling stage. The starch content in potatoes with mild water deficit in the seedling stage increased by 3.34% compared with that under full irrigation. In addition, the protein and vitamin C contents in potatoes under moderate water deficit increased significantly by 0.77% and 21.59%, respectively, compared with the contents under full irrigation. Principal component analysis identified mild water stress in the seedling stage as the optimum regulated deficit irrigation regime for potato. Thus, the relative soil water content should be maintained at 55% to 65% of field capacity in the seedling stage but at 65% to 75% in the other growth stages. The results of this study provide theoretical and technical references for efficient water-saving cultivation and industrialization of potato in cold and arid northwestern China.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d7c47159b9353d72292eed55fa60d78Test
https://pubmed.ncbi.nlm.nih.gov/34354149Test

المؤلفون: Yan Jing-cai, Shen Weijun, Muhammad Aziz ur Rahman, Wu Duanqin, Chen Dong, Tang Jing, Li Fuqiang

المصدر: Indian Journal of Animal Research.

مصطلحات موضوعية: Antioxidant, General Veterinary, medicine.diagnostic_test, medicine.medical_treatment, Hematocrit, Biology, Animal science, Immune system, Starter, medicine, Animal Science and Zoology, Hemoglobin, Thrombocytocrit, Lipoprotein, Hormone

الوصف: The objective of study was to evaluate the effects of energy level in starter ration on blood biochemical parameters, hormone profile, immune response and antioxidant status of calves. A total of eighteen angus calves were divided into three groups in such a way that each group had six calves. Three energy levels were {9 MJ/kg, low metabolizable energy (LME), 12.38 MJ/kg, medium metabolizable energy (MME) and 13.62 MJ/kg, high metabolizable energy (HME)}fed to experimental groups. Results showed that hemoglobin, red blood cells, hematocrit, thrombocytocrit and high-density lipoprotein was higher in HME diet (plessthan0.05). While, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, mean platelet volume and platelet distribution width was higher in MME diet as compared to HME diet plesstha0.05). Results of immunity revealed that IgA content were lowest in calves fed LME diet plesstha0.05). Calves received different energy level in the diet had no effect on IgG, IgM and C4 (P>0.05). However, the calves on LME starter diet represented higher blood complement C3 contents plesstha0.05). It is concluded that blood metabolites were influenced by energy level in Angus calves.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::834bd242a1312caaf76606fb9770b8c8Test
https://doi.org/10.18805/ijar.b-1163Test

المؤلفون: Wang Lili, Jiang Shan, Li Xiuli, Zheng Li, Yan Minghua, Li Fuqiang, Zhang Li, Lu Chao

المصدر: PLoS ONE
PLOS ONE
PLoS ONE, Vol 14, Iss 10, p e0223177 (2019)

مصطلحات موضوعية: 0301 basic medicine, Molecular biology, Swine, medicine.disease_cause, Biochemistry, Immune Receptors, Transcriptome, Sequencing techniques, Cell Signaling, Medicine and Health Sciences, Membrane Receptor Signaling, Immune Response, Coronavirus, Mammals, Swine Diseases, Multidisciplinary, Immune System Proteins, Eukaryota, RNA sequencing, General Medicine, Immune Receptor Signaling, Nucleic acids, Vertebrates, Medicine, General Agricultural and Biological Sciences, Coronavirus Infections, Research Article, Signal Transduction, Diarrhea, Science, 030106 microbiology, Immunology, Biology, Microbiology, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, Immune system, Immunity, Virology, medicine, Genetics, Animals, KEGG, Non-coding RNA, Gene, Natural antisense transcripts, Innate immune system, Gene Expression Profiling, Host Cells, Organisms, Biology and Life Sciences, Proteins, Cell Biology, Immunity, Innate, Gene regulation, Gene expression profiling, Research and analysis methods, MicroRNAs, 030104 developmental biology, Molecular biology techniques, Gene Expression Regulation, Amniotes, RNA, Gene expression, Viral Transmission and Infection

الوصف: Porcine deltacoronavirus (PDCoV) is a newly emerged swine enteropathogenic coronavirus affecting pigs of all ages and causing diarrhea problems. Research findings indicate that PDCoV has evolved strategies to escape innate immune response in host cells, but mechanism of PDCoV in innate immune modulation is not well understood. In this study, we report our findings on identifying the alterations of host cell innate immune response affected by PDCoV infection and exploring the gene expression profiles of PK-15 cells at 0, 24, and 36 h PDCoV post infection by RNA sequencing. A total of 3,762 and 560 differentially expressed genes (DEGs) were screened by comparison of uninfected PK-15 cells and infected PK-15 cells at 24 h post infection (hpi) (INF_24h versus NC), and also comparison of infected PK-15 cells between 24 and 36 hpi (INF_36h versus INF_24h), which included 156 and 23 porcine innate immune-related genes in the DEGs of INF_24h versus NC and INF_36h versus INF_24h, respectively. Gene Ontology function classification and Kyoto Encyclopedia of Genes and Genomes signaling pathway enrichment analysis were performed based on the DEGs that exhibited the same expression tendencies with most of the innate immune-associated genes among these PK-15 cell samples described above. The enrichment results indicated that extensive gene functions and signaling pathways including innate immune-associated functions and pathways were affected by PDCoV infection. Particularly, 4 of 5 innate immune signaling pathways, which were primarily affected by PDCoV, played important roles in I-IFN’s antiviral function in innate immune response. Additionally, 16 of the host cell endogenous miRNAs were predicted as potential contributors to the modulation of innate immune response affected by PDCoV. Our research findings indicated that the innate immune-associated genes and signaling pathways in PK-15 cells could be modified by the infection of PDCoV, which provides a fundamental foundation for further studies to better understand the mechanism of PDCoV infections, so as to effectively control and prevent PDCoV-induced swine diarrheal disease outbreaks.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c80249c8e9d5e2e2ee16c3ad18d19434Test
http://europepmc.org/articles/PMC6773216Test

المؤلفون: Li Fuqiang, Zhang Qinghua, Yin Yuhan, Yue Ziyun, Zhang Yongbo

المصدر: Revista da Sociedade Brasileira de Medicina Tropical, Volume: 49, Issue: 2, Pages: 165-171, Published: APR 2016
Revista da Sociedade Brasileira de Medicina Tropical, Vol 49, Iss 2, Pp 165-171 (2016)
Revista da Sociedade Brasileira de Medicina Tropical v.49 n.2 2016
Revista da Sociedade Brasileira de Medicina Tropical
Sociedade Brasileira de Medicina Tropical (SBMT)
instacron:SBMT

مصطلحات موضوعية: 0301 basic medicine, Microbiology (medical), Acinetobacter baumannii, lcsh:Arctic medicine. Tropical medicine, Klebsiella pneumoniae, lcsh:RC955-962, 030106 microbiology, Minocycline, Tigecycline, Drug resistance, Microbial Sensitivity Tests, Real-Time Polymerase Chain Reaction, Microbiology, 03 medical and health sciences, Minimum inhibitory concentration, Resistance mechanism, Drug Resistance, Bacterial, medicine, Humans, Klebsiella pneumonia, biology, Efflux pump, Broth microdilution, Gene Expression Regulation, Bacterial, biology.organism_classification, medicine.disease, Virology, Anti-Bacterial Agents, 030104 developmental biology, Infectious Diseases, Parasitology, Efflux, medicine.drug, Multilocus Sequence Typing

الوصف: INTRODUCTION: Due to the wide use of tigecycline in the treatment of severe infections caused by multidrug-resistant (MDR) bacteria, clinical resistance to tigecycline has increased in recent years. Here, we investigated the relationship between tigecycline resistance and the expression of efflux pumps. METHODS: Clinical isolates of Acinetobacter baumannii and Klebsiella pneumoniae were consecutively collected from hospitalized patients in three hospitals. The minimum inhibitory concentration (MIC) of tigecycline was determined using the broth microdilution method. Expression levels of efflux pump genes and regulators were examined by quantitative real-time reverse transcription polymerase chain reaction. The correlations between tigecycline MICs and gene expression levels were analyzed. RESULTS: Overall, 1,026 A. baumannii and 725 K. pneumoniae strains were collected. Most strains were isolated from sputum. The tigecycline resistance rate was 13.4% in A. baumannii isolates and 6.5% in K. pneumoniae isolates. Overexpression of AdeABC and AcrAB-TolC efflux systems was observed found in clinical tigecycline-resistant isolates. The tigecycline MIC had a linear relationship with the adeB expression level in A. baumannii isolates, but not with the acrB expression level in K. pneumoniae isolates. There were significant linear trends in the overexpression of ramA as the tigecycline MIC increased in K. pneumoniae isolates. CONCLUSIONS: Tigecycline resistance in A. baumannii and K. pneumoniae was strongly associated with the overexpression of efflux systems. More studies are needed to elucidate whether there are other regulators that affect the expression of adeB in A. baumannii and how ramA affects the expression of acrB in K. pneumoniae.

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الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::414bf69b89466f0ba7ab0aeec3663d68Test
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822016000200165&lng=en&tlng=enTest