Western-type diet influences mortality from necrotising pancreatitis and demonstrates a central role for butyrate
| العنوان: | Western-type diet influences mortality from necrotising pancreatitis and demonstrates a central role for butyrate |
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| المؤلفون: | Olga Zaborina, Sanjiv Hyoju, Marc G. Besselink, Demi van Dalen, John C. Alverdy, Fons F. van den Berg, Marja A. Boermeester, Hjalmar C. van Santvoort, Willem Joost Wiersinga |
| المساهمون: | AGEM - Amsterdam Gastroenterology Endocrinology Metabolism, Surgery, AII - Infectious diseases, Graduate School, Center of Experimental and Molecular Medicine, Infectious diseases |
| المصدر: | Gut Gut, 70(5):gutjnl-2019-320430, 915-927. BMJ Publishing Group |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | acute pancreatitis, medicine.drug_class, Antibiotics, Physiology, Butyrate, Gut flora, Systemic inflammation, antibiotics, Cecum, Mice, Immune system, medicine, Animals, Humans, Pancreas, biology, business.industry, Pancreatitis, Acute Necrotizing, Gastroenterology, bacterial infection, Fecal Microbiota Transplantation, biology.organism_classification, medicine.disease, butyrate, Gastrointestinal Microbiome, Mice, Inbred C57BL, Butyrates, Disease Models, Animal, medicine.anatomical_structure, Phenotype, Diet, Western, Disease Progression, Pancreatitis, Acute pancreatitis, medicine.symptom, business, diet |
| الوصف: | ObjectiveThe gut microbiota are the main source of infections in necrotising pancreatitis. We investigated the effect of disruption of the intestinal microbiota by a Western-type diet on mortality and bacterial dissemination in necrotising pancreatitis and its reversal by butyrate supplementation.DesignC57BL/6 mice were fed either standard chow or a Western-type diet for 4 weeks and were then subjected to taurocholate-induced necrotising pancreatitis. Blood and pancreas were collected for bacteriology and immune analysis. The cecum microbiota composition of mice was analysed using 16S rRNA gene amplicon sequencing and cecal content metabolites were analysed by targeted (ie, butyrate) and untargeted metabolomics. Prevention of necrotising pancreatitis in this model was compared between faecal microbiota transplantation (FMT) from healthy mice, antibiotic decontamination against Gram-negative bacteria and oral or systemic butyrate administration. Additionally, the faecal microbiota of patients with pancreatitis and healthy subjects were analysed.ResultsMortality, systemic inflammation and bacterial dissemination were increased in mice fed Western diet and their gut microbiota were characterised by a loss of diversity, a bloom ofEscherichia coliand an altered metabolic profile with butyrate depletion. While antibiotic decontamination decreased mortality, Gram-positive dissemination was increased. Both oral and systemic butyrate supplementation decreased mortality, bacterial dissemination, and reversed the microbiota alterations. Paradoxically, mortality and bacterial dissemination were increased with FMT administration. Finally, patients with acute pancreatitis demonstrated an increase in Proteobacteria and a decrease of butyrate producers compared with healthy subjects.ConclusionButyrate depletion and its repletion appear to play a central role in disease progression towards necrotising pancreatitis. |
| تدمد: | 1468-3288 0017-5749 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c5397e4e91fc00b9fe339ee78bf584e3Test https://pubmed.ncbi.nlm.nih.gov/32873697Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c5397e4e91fc00b9fe339ee78bf584e3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14683288 00175749 |
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