Early induction of a prechondrogenic population allows efficient generation of stable chondrocytes from human induced pluripotent stem cells
| العنوان: | Early induction of a prechondrogenic population allows efficient generation of stable chondrocytes from human induced pluripotent stem cells |
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| المؤلفون: | Jieun Lee, Fan Yang, William J. Maloney, Nidhi Bhutani, Piera Smeriglio, Sarah Taylor, Janice H. Lai |
| المصدر: | The FASEB Journal. 29:3399-3410 |
| بيانات النشر: | Wiley, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Adult, Cartilage, Articular, Male, Cellular differentiation, Induced Pluripotent Stem Cells, Population, Biology, Biochemistry, Extracellular matrix, Research Communication, Mice, Chondrocytes, Genetics, medicine, Animals, Humans, Regeneration, Induced pluripotent stem cell, education, Molecular Biology, Cells, Cultured, Stem cell transplantation for articular cartilage repair, education.field_of_study, Regeneration (biology), Cartilage, Cell Differentiation, SOX9 Transcription Factor, Anatomy, Chondrogenesis, Extracellular Matrix, Cell biology, HEK293 Cells, medicine.anatomical_structure, Female, Biotechnology |
| الوصف: | Regeneration of human cartilage is inherently inefficient; an abundant autologous source, such as human induced pluripotent stem cells (hiPSCs), is therefore attractive for engineering cartilage. We report a growth factor-based protocol for differentiating hiPSCs into articular-like chondrocytes (hiChondrocytes) within 2 weeks, with an overall efficiency >90%. The hiChondrocytes are stable and comparable to adult articular chondrocytes in global gene expression, extracellular matrix production, and ability to generate cartilage tissue in vitro and in immune-deficient mice. Molecular characterization identified an early SRY (sex-determining region Y) box (Sox)9low cluster of differentiation (CD)44lowCD140low prechondrogenic population during hiPSC differentiation. In addition, 2 distinct Sox9-regulated gene networks were identified in the Sox9low and Sox9high populations providing novel molecular insights into chondrogenic fate commitment and differentiation. Our findings present a favorable method for generating hiPSC-derived articular-like chondrocytes. The hiChondrocytes are an attractive cell source for cartilage engineering because of their abundance, autologous nature, and potential to generate articular-like cartilage rather than fibrocartilage. In addition, hiChondrocytes can be excellent tools for modeling human musculoskeletal diseases in a dish and for rapid drug screening.—Lee, J., Taylor, S. E. B., Smeriglio, P., Lai, J., Maloney, W. J., Yang, F., Bhutani, N. Early induction of a prechondrogenic population allows efficient generation of stable chondrocytes from human induced pluripotent stem cells. |
| تدمد: | 1530-6860 0892-6638 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::92c3a75f096adc20dfaf512ab3a4ab58Test https://doi.org/10.1096/fj.14-269720Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....92c3a75f096adc20dfaf512ab3a4ab58 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15306860 08926638 |
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