Over expression of AdeABC and AcrAB-TolC efflux systems confers tigecycline resistance in clinical isolates of Acinetobacter baumannii and Klebsiella pneumoniae
| العنوان: | Over expression of AdeABC and AcrAB-TolC efflux systems confers tigecycline resistance in clinical isolates of Acinetobacter baumannii and Klebsiella pneumoniae |
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| المؤلفون: | Li Fuqiang, Zhang Qinghua, Yin Yuhan, Yue Ziyun, Zhang Yongbo |
| المصدر: | Revista da Sociedade Brasileira de Medicina Tropical, Volume: 49, Issue: 2, Pages: 165-171, Published: APR 2016 Revista da Sociedade Brasileira de Medicina Tropical, Vol 49, Iss 2, Pp 165-171 (2016) Revista da Sociedade Brasileira de Medicina Tropical v.49 n.2 2016 Revista da Sociedade Brasileira de Medicina Tropical Sociedade Brasileira de Medicina Tropical (SBMT) instacron:SBMT |
| بيانات النشر: | Sociedade Brasileira de Medicina Tropical - SBMT, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Microbiology (medical), Acinetobacter baumannii, lcsh:Arctic medicine. Tropical medicine, Klebsiella pneumoniae, lcsh:RC955-962, 030106 microbiology, Minocycline, Tigecycline, Drug resistance, Microbial Sensitivity Tests, Real-Time Polymerase Chain Reaction, Microbiology, 03 medical and health sciences, Minimum inhibitory concentration, Resistance mechanism, Drug Resistance, Bacterial, medicine, Humans, Klebsiella pneumonia, biology, Efflux pump, Broth microdilution, Gene Expression Regulation, Bacterial, biology.organism_classification, medicine.disease, Virology, Anti-Bacterial Agents, 030104 developmental biology, Infectious Diseases, Parasitology, Efflux, medicine.drug, Multilocus Sequence Typing |
| الوصف: | INTRODUCTION: Due to the wide use of tigecycline in the treatment of severe infections caused by multidrug-resistant (MDR) bacteria, clinical resistance to tigecycline has increased in recent years. Here, we investigated the relationship between tigecycline resistance and the expression of efflux pumps. METHODS: Clinical isolates of Acinetobacter baumannii and Klebsiella pneumoniae were consecutively collected from hospitalized patients in three hospitals. The minimum inhibitory concentration (MIC) of tigecycline was determined using the broth microdilution method. Expression levels of efflux pump genes and regulators were examined by quantitative real-time reverse transcription polymerase chain reaction. The correlations between tigecycline MICs and gene expression levels were analyzed. RESULTS: Overall, 1,026 A. baumannii and 725 K. pneumoniae strains were collected. Most strains were isolated from sputum. The tigecycline resistance rate was 13.4% in A. baumannii isolates and 6.5% in K. pneumoniae isolates. Overexpression of AdeABC and AcrAB-TolC efflux systems was observed found in clinical tigecycline-resistant isolates. The tigecycline MIC had a linear relationship with the adeB expression level in A. baumannii isolates, but not with the acrB expression level in K. pneumoniae isolates. There were significant linear trends in the overexpression of ramA as the tigecycline MIC increased in K. pneumoniae isolates. CONCLUSIONS: Tigecycline resistance in A. baumannii and K. pneumoniae was strongly associated with the overexpression of efflux systems. More studies are needed to elucidate whether there are other regulators that affect the expression of adeB in A. baumannii and how ramA affects the expression of acrB in K. pneumoniae. |
| وصف الملف: | text/html |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::414bf69b89466f0ba7ab0aeec3663d68Test http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822016000200165&lng=en&tlng=enTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....414bf69b89466f0ba7ab0aeec3663d68 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |