Stress Regulates Aquaporin-8 Permeability to Impact Cell Growth and Survival
| العنوان: | Stress Regulates Aquaporin-8 Permeability to Impact Cell Growth and Survival |
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| المؤلفون: | Milena Bertolotti, Cinzia Bottino, Anna Rubartelli, Iria Medraño-Fernandez, Gerd Patrick Bienert, Umberto Laforenza, Roberto Sitia, Stefano Bestetti |
| المساهمون: | Medraño Fernandez, I, Bestetti, S, Bertolotti, M, Bienert, G, Bottino, C, Laforenza, U, Rubartelli, A, Sitia, R, Bienert, Gp, Sitia, Roberto |
| المصدر: | Antioxidants & Redox Signaling. 24:1031-1044 |
| بيانات النشر: | Mary Ann Liebert Inc, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Redox signaling, Cell Membrane Permeability, Cell Survival, Physiology, medicine.medical_treatment, Clinical Biochemistry, Gene Expression, Aquaporin, Saccharomyces cerevisiae, Biology, Aquaporins, medicine.disease_cause, Biochemistry, 03 medical and health sciences, medicine, Humans, Molecular Biology, Cell damage, Cell Proliferation, General Environmental Science, oxidative stre, Water transport, Cell growth, Growth factor, Water, Biological Transport, Biological membrane, Hydrogen Peroxide, Cell Biology, medicine.disease, Cell biology, Oxidative Stress, Original Research Communications, 030104 developmental biology, Unfolded protein response, General Earth and Planetary Sciences, Oxidation-Reduction, Oxidative stress, HeLa Cells |
| الوصف: | Aquaporin-8 (AQP8) allows the bidirectional transport of water and hydrogen peroxide across biological membranes. Depending on its concentration, H2O2 exerts opposite roles, amplifying growth factor signaling in physiological conditions, but causing severe cell damage when in excess. Thus, H2O2 permeability is likely to be tightly controlled in living cells. Aims: In this study, we investigated whether and how the transport of H2O2 through plasma membrane AQP8 is regulated, particularly during cell stress. Results: We show that diverse cellular stress conditions, including heat, hypoxia, and ER stress, reversibly inhibit the permeability of AQP8 to H2O2 and water. Preventing the accumulation of intracellular reactive oxygen species (ROS) during stress counteracts AQP8 blockade. Once inhibition is established, AQP8-dependent transport can be rescued by reducing agents. Neither H2O2 nor water transport is impaired in stressed cells expressing a mutant AQP8, in which cysteine 53 had been replaced by serine. Cells expressing this mutant are more resistant to stress-, drug-, and radiation-induced growth arrest and death. Innovation and Conclusion: The control of AQP8-mediated H2O2 transport provides a novel mechanism to regulate cell signaling and survival during stress. Antioxid. Redox Signal. 24, 1031–1044. |
| تدمد: | 1557-7716 1523-0864 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b443367cab828c76e25a3491eedcd412Test https://doi.org/10.1089/ars.2016.6636Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b443367cab828c76e25a3491eedcd412 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15577716 15230864 |
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