Aquaporin-3 regulates endosome-to-cytosol transfer via lipid peroxidation for cross presentation
| العنوان: | Aquaporin-3 regulates endosome-to-cytosol transfer via lipid peroxidation for cross presentation |
|---|---|
| المؤلفون: | Sebastian J. Fleire, George Posthuma, Judith Klumperman, Matthew L. Albert, Cecile Chalouni, E. Sergio Trombetta, Alan S. Verkman, Ira Mellman, Min Xu, Adriana Baz Morelli, Rosa Barreira da Silva, Ann De Mazière, Hua Zhang, Markus Decker, Sam C. Nalle |
| المصدر: | PLoS ONE PLoS ONE, Vol 15, Iss 11, p e0238484 (2020) |
| بيانات النشر: | Cold Spring Harbor Laboratory, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Endocytic cycle, Biochemistry, Gene Knockout Techniques, Mice, White Blood Cells, Cytosol, Spectrum Analysis Techniques, Animal Cells, Phagosomes, Medicine and Health Sciences, Cells, Cultured, Antigen Presentation, Innate Immune System, Multidisciplinary, biology, T Cells, Chemistry, Cross-presentation, Flow Cytometry, Lipids, Cell biology, Spectrophotometry, Medicine, Cytophotometry, Cellular Types, Cellular Structures and Organelles, Research Article, Endosome, Science, Immune Cells, Antigen presentation, Immunology, Antigen-Presenting Cells, Cytotoxic T cells, Endosomes, Research and Analysis Methods, Transfection, Endocytosis, Antigen, MHC class I, Animals, Humans, Vesicles, Antigen-presenting cell, Molecular Biology Techniques, Molecular Biology, Aquaporin 3, Blood Cells, Endoplasmic reticulum, Biology and Life Sciences, Biological Transport, Cell Biology, HEK293 Cells, Immune System, biology.protein, Lipid Peroxidation |
| الوصف: | Antigen cross presentation, whereby exogenous antigens are presented by MHC class I molecules to CD8+ T cells, is essential for generating adaptive immunity to pathogens and tumor cells (1). Following endocytosis, it is widely understood that protein antigens must be transferred from endosomes to the cytosol where they are subject to ubiquitination and proteasome degradation prior to being translocated into the endoplasmic reticulum (ER), or possibly endosomes, via the TAP1/TAP2 complex (2, 3). Revealing how antigens egress from endocytic organelles (endosome-to-cytosol transfer, ECT), however, has proved vexing. Here, we used two independent screens to identify the hydrogen peroxide-transporting channel aquaporin-3 (AQP3) as a regulator of ECT. AQP3 overexpression increased ECT, whereas AQP3 knockout or knockdown decreased ECT. Mechanistically, AQP3 appears to be important for hydrogen peroxide entry into the endosomal lumen where it affects lipid peroxidation and subsequent antigen release. AQP3-mediated regulation of ECT was functionally significant, as AQP3 modulation had a direct impact on the efficiency of antigen cross presentation in vitro. Finally, AQP3-/- mice exhibited a reduced ability to mount an anti-viral response and cross present exogenous extended peptide. Together, these results indicate that the AQP3-mediated transport of hydrogen peroxide can regulate endosomal lipid peroxidation and suggest that compromised membrane integrity and coordinated release of endosomal cargo is a likely mechanism for ECT. |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a10468bf335f7a8304dee087a46f61aaTest https://doi.org/10.1101/2020.08.19.256966Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a10468bf335f7a8304dee087a46f61aa |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |