Genetic screen in Drosophila muscle identifies autophagy-mediated T-tubule remodeling and a Rab2 role in autophagy
| العنوان: | Genetic screen in Drosophila muscle identifies autophagy-mediated T-tubule remodeling and a Rab2 role in autophagy |
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| المؤلفون: | Jean Francois Groulx, Naonobu Fujita, Noboru Mizushima, Mitsunori Fukuda, Yoshihiko Kuchitsu, Ikuko Koyama-Honda, Tzu Han Lin, Steve Jean, Wilson Huang, Amy A. Kiger, Jen Nguyen |
| المصدر: | eLife, Vol 6 (2017) eLife |
| بيانات النشر: | eScholarship, University of California, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Autophagosome, Membrane Fusion, T-tubule, R-SNARE Proteins, Phagosomes, cell biology, Morphogenesis, Myocyte, Drosophila Proteins, Protein Isoforms, Developmental, RNA, Small Interfering, Biology (General), Qa-SNARE Proteins, General Neuroscience, Muscles, Gene Expression Regulation, Developmental, muscle fiber, General Medicine, Muscle atrophy, Cell biology, Protein Transport, medicine.anatomical_structure, Drosophila melanogaster, Medicine, medicine.symptom, SNARE Proteins, Research Article, Signal Transduction, autophagy, QH301-705.5, Science, 1.1 Normal biological development and functioning, Biology, Small Interfering, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, developmental biology, Downregulation and upregulation, stem cells, Underpinning research, medicine, Animals, General Immunology and Microbiology, autophagosome-lysosome fusion, Gene Expression Profiling, Autophagy, rab7 GTP-Binding Proteins, Cell Biology, rab2 GTP-Binding Protein, 030104 developmental biology, Developmental Biology and Stem Cells, Gene Expression Regulation, Drosophila metamorphosis, rab GTP-Binding Proteins, RNA, Biochemistry and Cell Biology, Lysosomes, Developmental biology, Rab GTPase, Genetic screen |
| الوصف: | Transverse (T)-tubules make-up a specialized network of tubulated muscle cell membranes involved in excitation-contraction coupling for power of contraction. Little is known about how T-tubules maintain highly organized structures and contacts throughout the contractile system despite the ongoing muscle remodeling that occurs with muscle atrophy, damage and aging. We uncovered an essential role for autophagy in T-tubule remodeling with genetic screens of a developmentally regulated remodeling program in Drosophila abdominal muscles. Here, we show that autophagy is both upregulated with and required for progression through T-tubule disassembly stages. Along with known mediators of autophagosome-lysosome fusion, our screens uncovered an unexpected shared role for Rab2 with a broadly conserved function in autophagic clearance. Rab2 localizes to autophagosomes and binds to HOPS complex members, suggesting a direct role in autophagosome tethering/fusion. Together, the high membrane flux with muscle remodeling permits unprecedented analysis both of T-tubule dynamics and fundamental trafficking mechanisms. DOI: http://dx.doi.org/10.7554/eLife.23367.001Test |
| وصف الملف: | application/pdf |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::86f18e6be0f411d8bae6ea507a529193Test https://escholarship.org/uc/item/6wm4b7r5Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....86f18e6be0f411d8bae6ea507a529193 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |