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1دورية أكاديمية
المؤلفون: Mikael Luiz Pereira Morales, Gabrielle Paulsen Figurelli, Bárbara Oleinski, Grasiela Lopes Leães Pinho, Ng Haig They, Vanessa Ochi Agostini
مصطلحات موضوعية: Biochemistry, Microbiology, Pharmacology, Biotechnology, Ecology, Infectious Diseases, Virology, Environmental Sciences not elsewhere classified, Astronomical and Space Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Aquatic plants, antibiofilm activity, bacterial adhesion, biofouling, natural compounds
الوصف: Ecologically safe antifouling solutions have received growing attention since the acknowledgment of the environmental risks of traditional biocides used currently. Given that bacteria constitute the initial stage of the biofouling process, this work aims to evaluate the antifouling potential of aquatic macrophyte extracts in inhibiting biofilm-forming bacteria and to evaluate their ecotoxicological effects on target and nontarget organisms ( Thalassiosira pseudonana and Nitokra sp.). For this, the ability of 25 macrophyte extracts to inhibit and eradicate single and multispecies estuarine biofilms was evaluated. The extracts and respective serial dilutions that showed a biofilm inhibitory effect ≥ 60% were evaluated in ecotoxicological assays. Of the 25 extracts, only 6 extracts inhibited the biofilm ≥ 60%. The extracts of Cabomba caroliniana and Schoenoplectus californicus stood out as having a biofilm inhibitory effect ≥ 80% ( p < 0.05) for bacterial isolates and ≥ 70% ( p < 0.05) for multispecies biofilms. Furthermore, these extracts showed no toxic effects on nontarget organisms. These results demonstrate the biotechnological potential of macrophyte compounds.
الإتاحة: https://doi.org/10.6084/m9.figshare.25305688.v1Test
https://figshare.com/articles/journal_contribution/Antifouling_activity_of_aquatic_macrophyte_extracts_on_estuarine_bacterial_biofilms/25305688Test -
2دورية أكاديمية
المؤلفون: Soniya Rayi, Yanyao Cai, Jennifer L. Greenwich, Clay Fuqua, Joseph P. Gerdt
مصطلحات موضوعية: Biochemistry, Microbiology, Pharmacology, Ecology, Marine Biology, Infectious Diseases, Virology, Astronomical and Space Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, genetic knockout experiments, interbacterial biofilm competition, species biofilm regulation, inhibiting biofilm formation, siderophores still secreted, pseudomonas aeruginosa inhibits, species biofilm, biofilm formation, tumefaciens <, n <, fohorn ), dihydroaeruginoic acid, compensatory overproduction, chemical nature, chemical foundation, antibiofilm metabolite, antagonistic interactions, agrobacterium tumefaciens, >< sup
الوصف: Polymicrobial biofilms are ubiquitous, and the complex interspecies interactions within them are cryptic. We discovered the chemical foundation of antagonistic interactions in a model dual-species biofilm in which Pseudomonas aeruginosa inhibits the biofilm formation of Agrobacterium tumefaciens. Three known siderophores produced by P. aeruginosa (pyoverdine, pyochelin, and dihydroaeruginoic acid) were each capable of inhibiting biofilm formation. Surprisingly, a mutant that was incapable of producing these siderophores still secreted an antibiofilm metabolite. We discovered that this inhibitor was N 5 -formyl- N 5 -hydroxy-l-ornithine (fOHOrn)a precursor in pyoverdine biosynthesis. Unlike the siderophores, this inhibitor did not appear to function via extracellular metal sequestration. In addition to this discovery, the compensatory overproduction of a new biofilm inhibitor illustrates the risk of pleiotropy in genetic knockout experiments. In total, this work lends new insight into the chemical nature of dual-species biofilm regulation and reveals a new naturally produced inhibitor of A. tumefaciens biofilm formation.
الإتاحة: https://doi.org/10.1021/acschembio.3c00655.s001Test
https://figshare.com/articles/journal_contribution/Interbacterial_Biofilm_Competition_through_a_Suite_of_Secreted_Metabolites/25049789Test -
3دورية أكاديمية
المؤلفون: Pradisha Prem, Selvam Naveenkumar, Chinnaperumal Kamaraj, Chinnasamy Ragavendran, Arumugam Priyadharsan, Kumar Manimaran, Naify S Alharbi, Nilesh Rarokar, Tijo Cherian, Vimal Sugumar, Muthu Thiruvengadam, Vinoth Kumarasamy, Vetriselvan Subramaniyan
مصطلحات موضوعية: Biochemistry, Medicine, Microbiology, Biotechnology, Ecology, Infectious Diseases, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Bio-synthesis, Valeriana jatamansi, photocatalyst, antibiofilm, antioxidant
الوصف: This study aimed to synthesize silver nanoparticles (VJ@AgNPs) using Valeriana jatamansi root extract and assess their antibacterial, antioxidant, and antibiofilm properties against Escherichia coli , Staphylococcus aureus , and Streptococcus mutans . Various chemical and physical characterization methods were employed to analyze the synthesized VJ@AgNPs. UV-Visible spectroscopy confirmed the presence of VJ@AgNPs with a peak absorption at 426 nm, while FT-IR results indicated the involvement of phyto-compounds from V. jatamansi in the reduction and stabilization of these nanoparticles. High-resolution transmission electron microscopy revealed well-dispersed spherical nature with an average size of 29.1 ± 2.06 nm, and X-ray diffraction confirmed their crystalline structure. The biosynthesized VJ@AgNPs exhibited significant antibacterial activity against all tested pathogens, with substantial inhibition zones at 50 and 100 μg/mL concentrations. Additionally, VJ@AgNPs displayed potent antibiofilm activity and antioxidant capacity in scavenging assays. Furthermore, these VJ@AgNPs showed promise in photocatalytic degradation, effectively removing 95% of RhB dye within 75 minutes under solar light irradiation, following pseudo-first-order kinetics. This suggests their potential application in wastewater treatment for organic dye removal. The biocompatible and environmentally friendly nature of VJ@AgNPs underscores their potential as therapeutic agents against bacterial infections and oxidative stress-related diseases.
الإتاحة: https://doi.org/10.6084/m9.figshare.25133880.v1Test
https://figshare.com/articles/journal_contribution/_i_Valeriana_jatamansi_i_root_extract_a_potent_source_for_biosynthesis_of_silver_nanoparticles_and_their_biomedical_applications_and_photocatalytic_decomposition/25133880Test -
4دورية أكاديمية
مصطلحات موضوعية: Biochemistry, Microbiology, Pharmacology, Biotechnology, Ecology, Infectious Diseases, Virology, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Antimicrobial activity, Antibiofilm activity, Eucalyptus camaldulensis, Eucalyptus globulus, Volatile oil, Phytochemistry
الوصف: Eucalyptus is a fragrant plant with a highly volatile oil content grown worldwide. This study investigated the phytochemical and biological features of two Eucalyptus species, E. camaldulensis and E. globulus , cultivated in Central Saudi Arabia. Phytochemical analysis revealed 17 compounds in E. camaldulensis and 13 in E. globulus , with α-pinene and 1,8-cineole being both plants’ most abundant volatile components. The antimicrobial activity revealed that Salmonella typhimurium ATCC 13311 and Candida albicans ATCC 10231 are the most susceptible to E. camaldulensis , with the zone of inhibition diameters (ZIDs) of 17.0 ± 0.2 mm and 22.0 ± 0.3 mm, respectively. In contrast, for E. globulus , methicillin-resistant Staphylococcus aureus and Candida albicans ATCC 10231 were the most susceptible, with ZIDs of 13.0 ± 0.2 mm and 20.0 ± 0.2 mm, respectively. E. camaldulensis had minimum inhibitory concentration (MIC) range from 1.56 to 12.5 μL/mL, minimum biocidal concentration (MBC) range from 3.125 to 25.0 μL/mL, minimum biofilm inhibitory concentration (MBIC) range from 3.125 to 25.0 μL/mL, and minimum biofilm eradication concentration (MBEC) from 6.25 to 50.0 μL/mL, while E. globulus had MIC range from 3.125 to 12.5 μL/mL, MBC range from 6.25 to 25.0 μL/mL, MBIC range from 6.25 to 25.0 μL/mL, and MBEC range from 12.5 to 50.0 μL/mL, for the tested organisms. E. camaldulensis demonstrated better antimicrobial activity than E. globulus against the tested organisms, making it a promising antimicrobial drug candidate. Further research is required to determine its effectiveness in treating different human infections.
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5دورية أكاديمية
المؤلفون: Anmiya Peter, Sreelakshmi Sadanandan, E.S. Bindiya, Nivedya Mohan, Sarita G Bhat, K Abhitha
المصدر: Carbohydrate Polymer Technologies and Applications, Vol 6, Iss , Pp 100357- (2023)
مصطلحات موضوعية: Anisotropy, Silver nanoparticles, Antibacterial activity, Crystal violet assay, Antibiofilm activity, Biochemistry, QD415-436
الوصف: Intense bacterial infections have evolved into an immediate public and clinical health crisis, and medical devices are demanded to treat biofilm-mediated infections. It remains a challenge in medical products to eliminate pathogenic organisms despite documented protocols for cleaning and sanitizing surfaces. Silver nanoparticles have appeared as a favourable prospect to fight the rise of bacteria. The present article depicts the synthesis of carboxymethyl chitosan (CMC) stabilised anisotropic silver nanoparticles and their antimicrobial and antibiofilm activities. The dichlorofluorescein diacetate (DCF-DA) test against Staphylococcus aureus authenticated the reactive oxygen species (ROS) mediated antibacterial action of the nanoparticle. The silver nanoparticles were coated on natural rubber, and the surface properties of silver attached natural rubber developed by simple immersion were assessed using elemental analysis and inductively coupled plasma mass-spectrometry. The coated rubber film exhibits antibacterial properties with significant biofilm removal of 50.64% E. coli, 40.28% S. aureus and 33.87% P. aeruginosa with lower bacterial adhesion along with contact killing efficiency. The developed material can be used in rubber-based medical devices; thus provides a protective shield against bacteria, especially in the hospital field.
وصف الملف: electronic resource
العلاقة: http://www.sciencedirect.com/science/article/pii/S2666893923000786Test; https://doaj.org/toc/2666-8939Test
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6دورية أكاديمية
المؤلفون: Gamal M. El-Sherbiny, Ahmed Mohamed Gazelly, Mohammed H. Sharaf, Saad A. Moghannemm, Shehata M. E, Mahmoud K.A. Ismail, Ahmad S. El-Hawary
المصدر: Journal of Bioresources and Bioproducts, Vol 8, Iss 1, Pp 59-65 (2023)
مصطلحات موضوعية: β-lactam resistance Streptococcus species, S. persica extract, Antibacterial, Antibiofilm, Antioxidant, GC-MS analysis, Biochemistry, QD415-436
الوصف: The Salvadora persica (S. persica) L. chewing stick, usually known as miswak, is still being employed as an oral hygiene agent for plaque and gingivitis prevention. This study aims to assess the antibacterial, antibiofilm, antioxidant, and phytochemical profile of S. persica extract. The S. persica was purchased from a local market, grinded and extracted with petroleum ether. The disk diffusion, microdilution, and micro-plate assays were performed to evaluate the antibacterial and antibiofilm activities of the prepared extract at different concentrations against β-lactam resistance Streptococcus species. Free radical scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) and stable radical cationic chromophore, 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) methods were used to determine their antioxidant activity. Chromatographic and spectrometric analyses were performed using gas chromatography-mass (GC-MS) spectrometry. The minimum inhibitory concentration (MIC) of S. persica extract against β-lactam resistance Streptococcus species ranged from 6.25 to 12.5 mg/mL. The maximum suppression of biofilm formation by S. persica extract was observed at MIC with a percentage of 68.66%, against Streptococcus oralis. The S. persica extract exhibited antioxidant activity with IC50 of 20 µg/mL and 35 µg/mL from DPPH and ABTS, respectively. The phytochemical characterization showed the presence of 22 compounds with major compounds; benzyl isothiocyanate (36.21%) and n-hexadecanoic acid (27.62%). The S. persica extract exhibited antibacterial activity against β-lactam resistant Streptococcus species, showing a promising natural alternative that could be a treatment option.
وصف الملف: electronic resource
العلاقة: http://www.sciencedirect.com/science/article/pii/S2369969822000743Test; https://doaj.org/toc/2369-9698Test
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7دورية أكاديمية
المؤلفون: Gürkan Alkan, Seyhan Ulusoy, Mert Akgün, Ayhan Oral
المصدر: Macedonian Journal of Chemistry and Chemical Engineering, Vol 42, Iss 1 (2023)
مصطلحات موضوعية: Ibuprofen-Na, Paracetamol, Nanofiber, Antibiofilm, Antibacterial, Chemical engineering, TP155-156, Biochemistry, QD415-436
الوصف: Nanofibers loaded with pharmaceutical agents for various medical purposes have become more important in recent years because of their advantages, such as control on release, gas permeability, high surface area, and lightweight matrices. In the present study, polylactic acid (PLA)-gelatin (Gel) nanofibers were successfully loaded with Ibuprofen-Na/Paracetamol (henceforth Ibu-Na and Par, respectively) by electrospinning. The nanofibers were characterized by scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectroscopy. The Ibu-Na/Par content of the nanofibers was determined by using high-performance liquid chromatography (HPLC). Their antibacterial activities were tested against Enterococcus faecalis ATCC 29212, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa PA01, opportunistic pathogenic bacteria which are frequently associated with infections. Moreover, their antibiofilm activities against P. aeruginosa and E. faecalis were also investigated. The Ibu-Na-containing nanofibers exhibited antibacterial activity against S. aureus, E. coli, and E. faecalis. The inhibition zone diameters of PLA-Gel-Ibu-Na 300 against E. faecalis, S. aureus, and E. coli were calculated to be 23.0 ± 2.1 mm, 18.0 ± 1.5 mm, and 12.0 ± 1.2 mm, respectively. It was found that PLA-Gel-Ibu-Na 300 and PLA-Gel-Par 300 nanofibers’ capacity to show biofilm formation inhibition originated remarkable effects on P. aeruginosa, which were found to be 48 % and 50.4 %, respectively. This study indicated that Ibu-Na/Par-loaded nanofibers are promising materials for wound healing applications.
وصف الملف: electronic resource
العلاقة: https://mjcce.org.mk/index.php/MJCCE/article/view/2584Test; https://doaj.org/toc/1857-5552Test; https://doaj.org/toc/1857-5625Test
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8دورية أكاديمية
المؤلفون: Nilton A. S. Neto, Tawanny K. B. Aguiar, Rayara J. P. Costa, Felipe P. Mesquita, Lais L. B. de Oliveira, Maria E. A. de Moraes, Raquel C. Montenegro, Rômulo F. Carneiro, Celso S. Nagano, Cleverson D. T. Freitas, Pedro F. N. Souza
مصطلحات موضوعية: Biochemistry, Medicine, Microbiology, Cell Biology, Pharmacology, Biotechnology, Ecology, Cancer, Infectious Diseases, Virology, Chemical Sciences not elsewhere classified, Mechanisms of antibiofilm action, proteomic analysis, antibiofilm synthetic peptide
الوصف: Staphylococcus aureus forms biofilms, a structure that protects bacterial cells, conferring more resistance to difficult treatment. Synthetic peptides surge as an alternative to overcome the biofilm of multidrug-resistant pathogens. Mo -CBP 3 -PepI, when combined with Ciprofloxacin, reduced preformed S. aureus biofilm by 50% at low concentrations (0.2 and 6.2 μg. mL −1 , respectively). The goal of this study was to evaluate the proteomic profile of biofilms after treatment with the Mo -CBP 3 -PepI combined with ciprofloxacin. Here, proteomic analysis confirmed with more depth previously described mechanisms and revealed changes in the accumulation of proteins related to DNA and protein metabolism, cell wall biosynthesis, redox metabolism, quorum sensing, and biofilm formation. Some proteins related to DNA and protein metabolism were reduced, while other proteins, like redox system proteins, disappeared in Ciprofloxacin+ Mo -CBP 3 -PepI treatment. Our results indicated a synergistic effect of these two molecules with several mechanisms against S. aureus biofilm and opened new doors for combined treatments with other drugs.
الإتاحة: https://doi.org/10.6084/m9.figshare.24551571.v1Test
https://figshare.com/articles/journal_contribution/United_we_stand_divided_we_fall_in-depth_proteomic_evaluation_of_the_synergistic_effect_of_i_Mo_i_-CBP_sub_3_sub_-PepI_and_Ciprofloxacin_against_i_Staphylococcus_aureus_i_biofilms/24551571Test -
9دورية أكاديمية
المؤلفون: Nalova Ikome, Hermia, Tamfu, Alfred, Abdou, Jean, Fouotsa, Hugues, Nangmo, Pamela, Castro, Fidèle, Lah, Weyepe, Tchinda, Alembert, Ceylan, Ozgur, Frederich, Michel, Nkengfack, Augustin
المصدر: Applied Biochemistry and Biotechnology, 195 (10), 6113-6131 (2023-02-22)
مصطلحات موضوعية: Zanthoxylum gilletii, Secondary metabolites, Antimicrobial activity, Antibiofilm, Anti-quorum sensing, Molecular Biology, Applied Microbiology and Biotechnology, Biochemistry, General Medicine, Bioengineering, Biotechnology, Human health sciences, Pharmacy, pharmacology & toxicology, Sciences de la santé humaine, Pharmacie, pharmacologie & toxicologie
الوصف: Microbial resistance is facilitated by biofilm formation and quorum-sensing mediated processes. In this work, the stem bark (ZM) and fruit extracts (ZMFT) of Zanthoxylum gilletii were subjected to column chromatography and afforded lupeol (1), 2,3-epoxy-6,7-methylenedioxyconiferyl alcohol (3), nitidine chloride (4), nitidine (7), sucrose (6) and sitosterolβ-D-glucopyranoside (2). The compounds were characterized using MS and NMR spectral data. The samples were evaluated for antimicrobial, antibiofilm and anti-quorum sensing activities. Highest antimicrobial activity was exhibited by compounds 3, 4 and 7 against Staphylococcus aureus (MIC 200 µg/mL), compounds 3 and 4 against Escherichia coli (MIC = 100 µg/mL) and compounds 4 and 7 against Candida albicans (MIC = 50 µg/mL). At MIC and sub-MIC concentrations, all samples inhibited biofilm formation by pathogens and violacein production in C. violaceum CV12472 except compound 6. Good disruption of QS-sensing in C. violaceum revealed by inhibition zone diameters were exhibited by compounds 3 (11.5 ± 0.5 mm), 4 (12.5 ± 1.5 mm), 5 (15.0 ± 0.8 mm), 7 (12.0 ± 1.5 mm) as well as the crude extracts from stem barks (16.5 ± 1.2 mm) and seeds (13.0 ± 1.4 mm). The profound inhibition of quorum sensing mediated processes in test pathogens by compounds 3, 4, 5 and 7 suggests the methylenedioxy-group that these compounds possess as the possible pharmacophore.
العلاقة: https://link.springer.com/content/pdf/10.1007/s12010-023-04380-6.pdfTest; urn:issn:0273-2289; urn:issn:1559-0291
الوصول الحر: https://orbi.uliege.be/handle/2268/313729Test
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10دورية أكاديمية
المؤلفون: Tan Xiuchuan, Xie Shenghong, Jin Xiao, Zhu Ruiying, Wei Shuangshuang, Song Yanting, Xie Xi, Wang Rong, Li Lushuang, Wang Manchuriga, Zhang Yingxia
المصدر: Acta Biochimica et Biophysica Sinica, Vol 54, Pp 350-360 (2022)
مصطلحات موضوعية: antimicrobial peptide, antibacterial activity, antibiofilm activity, methicillin-resistant Staphylococcus aureus, Biochemistry, QD415-436, Genetics, QH426-470
الوصف: Temporin-GHa (GHa) was cloned from Hylarana guentheri, showing a weak antimicrobial activity. In order to improve its bactericidal efficacy, GHaR6R, GHaR7R, GHaR8R and GHaR9W were designed and synthesized. Compared to the parent peptide, the GHa-derived peptides show potent antimicrobial activities against methicillin-resistant Staphylococcus aureus (MRSA), which is the main pathogen with high morbidity and mortality that causes various infections in humans. These peptides exert bactericidal actions on MRSA by permeabilizing the cytoplasmic membranes and damaging membrane integrity. All of the four peptides exhibit excellent stability under harsh conditions, including extreme temperature and salts. Furthermore, they inhibit the formation of biofilm and eradicate mature biofilm of MRSA. The GHa-derived peptides decrease bacterial surface hydrophobicity, autoaggregation and polysaccharide intercellular adhesion synthesis in concentration-dependent manner. Real-time quantitative reverse transcription PCR analysis revealed that the peptides downregulate the expression of adhesion genes icaADBC involved in biofilm formation. Except for GHaR7R, the other three peptides have low hemolytic toxicity against human erythrocytes. In the presence of human erythrocytes, GHaR7R, GHaR8R and GHaR9W interact with MRSA preferentially. GHaR6R, GHaR8R and GHaR9W show less toxicity toward normal cells HL-7702 and hFOB1.19. These results suggest that the GHa-derived peptides may be promising antimicrobial candidates against MRSA infections.
وصف الملف: electronic resource
العلاقة: https://doaj.org/toc/1672-9145Test
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