المؤلفون: Patricia H. Alvarenga, Thiago Luiz Alves e Silva, Motoshi Suzuki, Glenn Nardone, Pedro Cecilio, Joel Vega-Rodriguez, Jose M.C. Ribeiro, John F. Andersen

مصطلحات موضوعية: Biochemistry, Microbiology, Physiology, Ecology, Immunology, Inorganic Chemistry, Biological Sciences not elsewhere classified, free quantification approaches, families classically described, applied mass spectrometry, aedes aegypti <, 2 %, respectively, comprehensive proteomics analysis, also highly abundant, 102 serine proteases, based absolute quantification, serine protease, comprehensive catalogue, also provides, transports nutrients, relative abundances, proteins belonging, physiological importance, mosquito physiology, male mosquitoes, immune response, future studies, future research, disease transmission

الوصف: In arthropods, hemolymph carries immune cells and solubilizes and transports nutrients, hormones, and other molecules that are involved in diverse physiological processes including immunity, metabolism, and reproduction. However, despite such physiological importance, little is known about its composition. We applied mass spectrometry-based label-free quantification approaches to study the proteome of hemolymph perfused from sugar-fed female and male Aedes aegypti mosquitoes. A total of 1403 proteins were identified, out of which 447 of them were predicted to be extracellular. In both sexes, almost half of these extracellular proteins were predicted to be involved in defense/immune response, and their relative abundances (based on their intensity-based absolute quantification, iBAQ) were 37.9 and 33.2%, respectively. Interestingly, among them, 102 serine proteases/serine protease-homologues were identified, with almost half of them containing CLIP regulatory domains. Moreover, proteins belonging to families classically described as chemoreceptors, such as odorant-binding proteins (OBPs) and chemosensory proteins (CSPs), were also highly abundant in the hemolymph of both sexes. Our data provide a comprehensive catalogue of A. aegypti hemolymph basal protein content, revealing numerous unexplored targets for future research on mosquito physiology and disease transmission. It also provides a reference for future studies on the effect of blood meal and infection on hemolymph composition.

العلاقة: https://figshare.com/articles/journal_contribution/Comprehensive_Proteomics_Analysis_of_the_Hemolymph_Composition_of_Sugar-Fed_i_Aedes_aegypti_i_Female_and_Male_Mosquitoes/25342872Test

الإتاحة: https://doi.org/10.1021/acs.jproteome.3c00918.s001Test
https://figshare.com/articles/journal_contribution/Comprehensive_Proteomics_Analysis_of_the_Hemolymph_Composition_of_Sugar-Fed_i_Aedes_aegypti_i_Female_and_Male_Mosquitoes/25342872Test

المؤلفون: Micali, Giuseppe, Verzì, Anna Elisa, Musumeci, Maria Letizia, Nardone, Beatrice, Monfrecola, Giuseppe, Lacarrubba, Francesco

المصدر: Journal of Investigative Dermatology ; volume 143, issue 4, page 667-669.e2 ; ISSN 0022-202X

مصطلحات موضوعية: Cell Biology, Dermatology, Molecular Biology, Biochemistry

الإتاحة: https://doi.org/10.1016/j.jid.2022.10.003Test
https://api.elsevier.com/content/article/PII:S0022202X22026562?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0022202X22026562?httpAccept=text/plainTest

المؤلفون: Alvarenga, Patricia H., Alves e Silva, Thiago Luiz, Suzuki, Motoshi, Nardone, Glenn, Cecilio, Pedro, Vega-Rodriguez, Joel, Ribeiro, Jose M.C., Andersen, John F.

المساهمون: National Institute of Allergy and Infectious Diseases

المصدر: Journal of Proteome Research ; volume 23, issue 4, page 1471-1487 ; ISSN 1535-3893 1535-3907

مصطلحات موضوعية: General Chemistry, Biochemistry

الإتاحة: https://doi.org/10.1021/acs.jproteome.3c00918Test

المؤلفون: Clemente, Claudia, Johnson, Nicholas, Ouyang, Xiaodan, Popin, Rafael, Dall'Angelo, Sergio, Wahlsten, Matti, Jokela, Jouni, Colombano, Alessandro, Nardone, Brunello, Fewer, David P., Houssen, Wael E.

المساهمون: Institute of Biotechnology, Department of Food and Nutrition, Department of Microbiology, Faculty of Agriculture and Forestry, Cyanobacteria research, Helsinki Institute of Sustainability Science (HELSUS), Microbial Natural Products

مصطلحات موضوعية: DRUG DISCOVERY, CYCLIC PEPTIDE, MACROCYCLASE, HETEROCYCLIZATION, TRYPTOPHAN, PROTEINS, ENZYMES, ENABLES, Biochemistry, cell and molecular biology

الوصف: Cyanobactins are linear and cyclic post-translationally modified peptides. Here we show that the prenyl-D-Arg-containing autum-nalamide A is a member of the cyanobactin family. Biochemical assays demonstrate that the AutF prenyltransferase targets the guanidinium moiety in arginine and homoarginine and is a useful tool for biotechnological applications. ; Peer reviewed

وصف الملف: application/pdf

العلاقة: This project was supported by a fellowship grant from the EPSRC (No. EP/S027246/1, W.E.H.), a training grant from the BBSRC (BB/V509206/1, W. E. H. and S. D.), the Novo Nordisk Foundation (18OC0034838, D. P. F.) and the NordForsk NCoE program ``NordAqua'' (Project Number 82845, D. P. F.). C. C. is funded by a PhD studentship from University of Aberdeen. N. J. is funded by the IBioIC CTP PhD programme. R. V. P. was funded by the Doctoral Programme in Microbiology and Biotechnology of the University of Helsinki. X. O. was funded by the China Scholarship Council (Grant 201906150148). We are grateful to Professor James Naismith (University of Oxford) for sharing the construct used to express the macrocyclase PCY1. We thank Dr Huanting Liu (University of St Andrews) for sharing the pEHISTEV-SUMO vector.; Clemente , C , Johnson , N , Ouyang , X , Popin , R , Dall'Angelo , S , Wahlsten , M , Jokela , J , Colombano , A , Nardone , B , Fewer , D P & Houssen , W E 2022 , ' Biochemical characterization of a cyanobactin arginine-N-prenylase from the autumnalamide biosynthetic pathway ' , Chemical Communications , vol. 58 , no. 86 , pp. 12054-12057 . https://doi.org/10.1039/d2cc01799gTest; ORCID: /0000-0003-3978-4845/work/123135547; ORCID: /0000-0001-5096-3575/work/123136680; ORCID: /0000-0002-4107-1695/work/123138419; ORCID: /0000-0003-0427-0326/work/123143232; http://hdl.handle.net/10138/350678Test; 3b58f94b-ecb7-4af4-af19-d4b0750e3f24; 000863636300001

الإتاحة: http://hdl.handle.net/10138/350678Test

المؤلفون: Daftary, K.M., Feissinger, L., Nardone, B., Liszewski, W.

المصدر: Journal of Investigative Dermatology ; volume 142, issue 8, page B9 ; ISSN 0022-202X

مصطلحات موضوعية: Cell Biology, Dermatology, Molecular Biology, Biochemistry

الإتاحة: https://doi.org/10.1016/j.jid.2022.05.923Test
https://api.elsevier.com/content/article/PII:S0022202X22013641?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0022202X22013641?httpAccept=text/plainTest

المؤلفون: Emge, D.A., Liu, B., Green, C., Banez, L., Mauskar, M., Ziemer, C., Micheletti, R., Nutan, F., DeNiro, K., Mostaghimi, A., Keller, J., Nardone, B., Nguyen, C., Seminario-Vidal, L., Curtis, J., Madigan, L., R. deShazo, Cardones, A.

المصدر: Journal of Investigative Dermatology ; volume 142, issue 8, page S31 ; ISSN 0022-202X

مصطلحات موضوعية: Cell Biology, Dermatology, Molecular Biology, Biochemistry

الإتاحة: https://doi.org/10.1016/j.jid.2022.05.190Test
https://api.elsevier.com/content/article/PII:S0022202X22006315?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0022202X22006315?httpAccept=text/plainTest

المؤلفون: Samuel C. L. Smith (10912288), Carl Banbury (6559976), Davide Zardo (10912291), Rosanna Cannatelli (10912294), Olga M. Nardone (10912297), Uday N. Shivaji (10912300), Subrata Ghosh (124413), Pola Goldberg Oppenheimer (1303602), Marietta Iacucci (5227916)

مصطلحات موضوعية: Biochemistry, Medicine, Cell Biology, Biotechnology, Immunology, Space Science, Chemical Sciences not elsewhere classified, bowel disease Background Mucosal he., IBD patients, Raman Spectroscopy, 74 IBD patients, NPV, HC, inflammation, MH, 9700 Raman Spectra, UC, diagnosis endoscopic assessment, vivo RS analysis, vs, PPV, sd

الوصف: (DOCX)

العلاقة: https://figshare.com/articles/journal_contribution/Example_confusion_matrix_of_the_trained_predictive_model_at_differentiating_MH_from_active_CD_/14719192Test

الإتاحة: https://doi.org/10.1371/journal.pone.0252210.s002Test

المؤلفون: Samuel C. L. Smith (10912288), Carl Banbury (6559976), Davide Zardo (10912291), Rosanna Cannatelli (10912294), Olga M. Nardone (10912297), Uday N. Shivaji (10912300), Subrata Ghosh (124413), Pola Goldberg Oppenheimer (1303602), Marietta Iacucci (5227916)

مصطلحات موضوعية: Biochemistry, Medicine, Cell Biology, Biotechnology, Immunology, Space Science, Chemical Sciences not elsewhere classified, bowel disease Background Mucosal he., IBD patients, Raman Spectroscopy, 74 IBD patients, NPV, HC, inflammation, MH, 9700 Raman Spectra, UC, diagnosis endoscopic assessment, vivo RS analysis, vs, PPV, sd

الوصف: (DOCX)

العلاقة: https://figshare.com/articles/journal_contribution/Example_confusion_matrix_of_the_trained_predictive_model_at_differentiating_MH_from_active_UC_/14719189Test

الإتاحة: https://doi.org/10.1371/journal.pone.0252210.s001Test

المؤلفون: Lu, Stephen, Tirloni, Lucas, Oliveira, Markus Berger, Bosio, Christopher F., Nardone, Glenn A., Zhang, Yixiang, Hinnebusch, B. Joseph, Ribeiro, José M., Andersen, John F.

المساهمون: National Institute of Allergy and Infectious Diseases, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health

المصدر: Journal of Biological Chemistry ; volume 297, issue 5, page 101322 ; ISSN 0021-9258

مصطلحات موضوعية: Cell Biology, Molecular Biology, Biochemistry

الإتاحة: https://doi.org/10.1016/j.jbc.2021.101322Test
https://api.elsevier.com/content/article/PII:S0021925821011285?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0021925821011285?httpAccept=text/plainTest

المؤلفون: Ghafari, G., Liszewski, W., Nardone, B.

المصدر: Journal of Investigative Dermatology ; volume 141, issue 5, page S65 ; ISSN 0022-202X

مصطلحات موضوعية: Cell Biology, Dermatology, Molecular Biology, Biochemistry

الإتاحة: https://doi.org/10.1016/j.jid.2021.02.396Test
https://api.elsevier.com/content/article/PII:S0022202X21006217?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0022202X21006217?httpAccept=text/plainTest