Aberrant DNA methylation of the toll-like receptors 2 and 6 genes in patients with obstructive sleep apnea
| العنوان: | Aberrant DNA methylation of the toll-like receptors 2 and 6 genes in patients with obstructive sleep apnea |
|---|---|
| المؤلفون: | Mao Chang Su, Jen Chieh Chang, Huang Chih Chang, Yung-Che Chen, Ting Ya Wang, Chia Cheng Tseng, Chien Hung Chin, Yi Xin Zheng, Chang-Chun Hsiao, Kuo Tung Huang, Meng-Chih Lin, Yong Yong Lin |
| المصدر: | PLoS ONE, Vol 15, Iss 2, p e0228958 (2020) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Physiology, Protein Expression, Biochemistry, Immune Receptors, Epigenesis, Genetic, Mathematical and Statistical Techniques, 0302 clinical medicine, Medicine and Health Sciences, Medicine, Hypoxia, Promoter Regions, Genetic, Receptor, Toll-like Receptors, Sleep Apnea, Obstructive, DNA methylation, Immune System Proteins, Multidisciplinary, Continuous Positive Airway Pressure, Statistics, Middle Aged, Chromatin, Nucleic acids, Phenotype, CpG site, Physical Sciences, Regression Analysis, Epigenetics, Female, DNA modification, Chromatin modification, Research Article, Chromosome biology, Signal Transduction, Adult, Cell biology, medicine.medical_specialty, Polysomnography, Science, Immunology, Taiwan, Linear Regression Analysis, Research and Analysis Methods, Promoter Regions, 03 medical and health sciences, Internal medicine, Genetics, Gene Expression and Vector Techniques, Humans, Gene Regulation, Statistical Methods, Molecular Biology Techniques, Molecular Biology, Gene, Molecular Biology Assays and Analysis Techniques, Treatment Guidelines, Health Care Policy, Biology and life sciences, business.industry, Proteins, Promoter, DNA, medicine.disease, Toll-Like Receptor 2, Health Care, Obstructive sleep apnea, TLR2, Toll-Like Receptor 6, 030104 developmental biology, Endocrinology, Case-Control Studies, TLR6, CpG Islands, Gene expression, Physiological Processes, Sleep, business, Mathematics, 030217 neurology & neurosurgery |
| الوصف: | Obstructive sleep apnea (OSA) is a syndrome leading to chronic intermittent hypoxia, and the up-regulation of toll-like receptors (TLR) 2 and 6 on peripheral blood cells has been reported. We hypothesized that DNA methylation in TLR2 and TLR6 genes may play a role in the development of OSA and its excessive daytime sleepiness (EDS) phenotype. DNA methylation over 28 cytosine-phosphate-guanine (CpG) sites of the TLR2 promoter region and 3 CpG sites of the TLR6 gene body, and their protein expressions were measured by using pyrosequencing and ELISA methods in 18 heathy subjects (HS) and 58 patients with severe OSA (divided into 18 non-EDS and 40 EDS group). Patients with severe OSA had higher DNA methylation levels over five CpG sites (#1, #2, #3, #25 and #28) and lower DNA methylation levels over CpG site #18 of the TLR2 promoter region, higher DNA methylation levels over two CpG sites (#1 and #3) of the TLR6 gene body, and higher protein expressions of TLR6 than HS. The CpG site #2 of the TLR6 gene body was hypermethylated in severe OSA patients with EDS. Both DNA methylation levels over CpG site #1 of the TLR6 gene body and protein expressions of TLR6 were reduced after more than 6 months of nasal CPAP treatment in seven selected patients. Aberrant DNA methylation of the TLR2 promoter region and TLR6 gene body are associated with the consequence of severe OSA and its EDS phenotype. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::378cf76163d0c64833250224a14a8c81Test https://doaj.org/article/03b54890cc4e45a78054714c7c3fa980Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....378cf76163d0c64833250224a14a8c81 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
|---|