التفاصيل البيبلوغرافية
| العنوان: |
Extracellular matrix surface regulates self-assembly of three-dimensional placental trophoblast spheroids. |
| المؤلفون: |
Wong, Michael K.1,2, Shawky, Sarah A.2, Aryasomayajula, Aditya3, Green, Madeline A.2, Ewart, Tom4, Selvaganapathy, P. Ravi3, Raha, Sandeep1,2 rahas@mcmaster.ca |
| المصدر: |
PLoS ONE. 6/25/2018, Vol. 13 Issue 6, p1-18. 18p. |
| مصطلحات موضوعية: |
*EXTRACELLULAR matrix, *PLACENTAL extracts, *TROPHOBLASTIC tumors, *TROPHOBLAST, *CELL proliferation |
| مستخلص: |
The incorporation of the extracellular matrix (ECM) is essential for generating in vitro models that truly represent the microarchitecture found in human tissues. However, the cell-cell and cell-ECM interactions in vitro remains poorly understood in placental trophoblast biology. We investigated the effects of varying the surface properties (surface thickness and stiffness) of two ECMs, collagen I and Matrigel, on placental trophoblast cell morphology, viability, proliferation, and expression of markers involved in differentiation/syncytial fusion. Most notably, thicker Matrigel surfaces were found to induce the self-assembly of trophoblast cells into 3D spheroids that exhibited thickness-dependent changes in viability, proliferation, syncytial fusion, and gene expression profiles compared to two-dimensional cultures. Changes in F-actin organization, cell spread morphologies, and integrin and matrix metalloproteinase gene expression profiles, further reveal that the response to surface thickness may be mediated in part through cellular stiffness-sensing mechanisms. Our derivation of self-assembling trophoblast spheroid cultures through regulation of ECM surface alone contributes to a deeper understanding of cell-ECM interactions, and may be important for the advancement of in vitro platforms for research or diagnostics. [ABSTRACT FROM AUTHOR] |
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