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Full-Length Fibronectin Drives Fibroblast Accumulation at the Surface of Collagen Microtissues during Cell-Induced Tissue Morphogenesis

التفاصيل البيبلوغرافية
العنوان: Full-Length Fibronectin Drives Fibroblast Accumulation at the Surface of Collagen Microtissues during Cell-Induced Tissue Morphogenesis
المؤلفون: Foolen, Jasper, Shiu, Jau-Ye, Mitsi, Maria, Zhang, Yang, Chen, Christopher S., Vogel, Viola
بيانات النشر: Public Library of Science
سنة النشر: 2016
المجموعة: Harvard University: DASH - Digital Access to Scholarship at Harvard
مصطلحات موضوعية: Biology and Life Sciences, Biochemistry, Proteins, Collagens, Cell Biology, Cellular Types, Animal Cells, Connective Tissue Cells, Fibroblasts, Anatomy, Biological Tissue, Connective Tissue, Medicine and Health Sciences, Spectrum Analysis Techniques, Spectrophotometry, Fluorophotometry, Fluorescence Resonance Energy Transfer, Developmental Biology, Morphogenesis, Cell Motility, Cell Migration, Cell Adhesion, Integrins, Cellular Structures and Organelles, Extracellular Matrix, Tissue Distribution, Pharmacology, Pharmacokinetics, Physical Sciences, Materials Science
الوصف: Generating and maintaining gradients of cell density and extracellular matrix (ECM) components is a prerequisite for the development of functionality of healthy tissue. Therefore, gaining insights into the drivers of spatial organization of cells and the role of ECM during tissue morphogenesis is vital. In a 3D model system of tissue morphogenesis, a fibronectin-FRET sensor recently revealed the existence of two separate fibronectin populations with different conformations in microtissues, i.e. ‘compact and adsorbed to collagen’ versus ‘extended and fibrillar’ fibronectin that does not colocalize with the collagen scaffold. Here we asked how the presence of fibronectin might drive this cell-induced tissue morphogenesis, more specifically the formation of gradients in cell density and ECM composition. Microtissues were engineered in a high-throughput model system containing rectangular microarrays of 12 posts, which constrained fibroblast-populated collagen gels, remodeled by the contractile cells into trampoline-shaped microtissues. Fibronectin’s contribution during the tissue maturation process was assessed using fibronectin-knockout mouse embryonic fibroblasts (Fn-/- MEFs) and floxed equivalents (Fnf/f MEFs), in fibronectin-depleted growth medium with and without exogenously added plasma fibronectin (full-length, or various fragments). In the absence of full-length fibronectin, Fn-/- MEFs remained homogenously distributed throughout the cell-contracted collagen gels. In contrast, in the presence of full-length fibronectin, both cell types produced shell-like tissues with a predominantly cell-free compacted collagen core and a peripheral surface layer rich in cells. Single cell assays then revealed that Fn-/- MEFs applied lower total strain energy on nanopillar arrays coated with either fibronectin or vitronectin when compared to Fnf/f MEFs, but that the presence of exogenously added plasma fibronectin rescued their contractility. While collagen decoration of single fibronectin fibers enhanced the ...
نوع الوثيقة: article in journal/newspaper
وصف الملف: application/pdf
اللغة: English
تدمد: 1932-6203
العلاقة: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5001707/pdfTest/; PLoS ONE; Foolen, Jasper, Jau-Ye Shiu, Maria Mitsi, Yang Zhang, Christopher S. Chen, and Viola Vogel. 2016. “Full-Length Fibronectin Drives Fibroblast Accumulation at the Surface of Collagen Microtissues during Cell-Induced Tissue Morphogenesis.” PLoS ONE 11 (8): e0160369. doi:10.1371/journal.pone.0160369. http://dx.doi.org/10.1371/journal.pone.0160369Test.; http://nrs.harvard.edu/urn-3:HUL.InstRepos:29407769Test
DOI: 10.1371/journal.pone.0160369
الإتاحة: https://doi.org/10.1371/journal.pone.0160369Test
http://nrs.harvard.edu/urn-3:HUL.InstRepos:29407769Test
رقم الانضمام: edsbas.6C394FCF
قاعدة البيانات: BASE
الوصف
تدمد:19326203
DOI:10.1371/journal.pone.0160369