التفاصيل البيبلوغرافية
| العنوان: |
Molecular profiling and bioactive potential of an endophytic fungus Aspergillus sulphureus isolated from Sida acuta : a medicinal plant. |
| المؤلفون: |
Murali, M., Mahendra, C., Hema, P., Rajashekar, N., Nataraju, A., Sudarshana, M. S., Amruthesh, K. N. |
| المصدر: |
Pharmaceutical Biology; Dec2017, Vol. 55 Issue 1, p1623-1630, 8p |
| مصطلحات موضوعية: |
ASPERGILLUS, MALVACEAE, MEDICINAL plants, ENDOPHYTIC fungi, BIOACTIVE compounds |
| مستخلص: |
Context:Sidaacuta Burm.f. (Malvaceae) extracts are reported to have applications against malaria, diuretic, antipyretic, nervous and urinary diseases. No fungal endophytes ofS. acutaare reported. Objective:Isolation, identification and evaluation of antibacterial, antioxidant, anticancer and haemolytic potential of fungal endophytes from the ethnomedcinal plantS. acuta. Materials and methods:Sida acutastem segments were placed on PDA medium to isolate endophytic fungi. The fungus was identified by genomic DNA analysis and phylogenetic tree was constructed using ITS sequences (GenBank) to confirm species. The antibacterial efficacy ofAspergillus sulphureusMME12 ethyl acetate extract was tested against Gram-positive and Gram-negative pathogenic bacteria. DPPH free radical scavenging activity, anticancer and DNA fragmentation against EAC cells, and direct haemolytic activity (100–500 μg/mL) using human erythrocytes were determined. Results and discussion:The ethyl acetate extract ofA. sulphureus(Fresen.) Wehmer (Trichocomaceae) demonstrated significant antibacterial potential againstStaphylococcus aureus, Bacillus subtilis, Escherichia coliandSalmonella typhicompared to streptomycin. MIC against test pathogens was in the range of 15.6–62.5 μg/mL. The antioxidant results revealed significant RSA from 12.43% to 62.02% (IC50 = 350.4 μg/mL,p ≤ 0.05). MME12 offered considerable inhibition of EAC proliferation (23% to 84%, IC50 = 216.7 μg/mL,p ≤ 0.05) supported by DNA fragmentation studies. The extract also offered insignificant haemolysis (5.6%) compared to Triton X-100. Conclusions:A single endophytic fungus,A. sulphureusMME12 was isolated and identified using molecular profiling. The above-mentioned findings support the pharmacological application ofA. sulphureusMME12 extract and demand for purification of the active principle(s). [ABSTRACT FROM PUBLISHER] |
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| قاعدة البيانات: |
Complementary Index |
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