High throughput screening identifies SOX2 as a super pioneer factor that inhibits DNA methylation maintenance at its binding sites
| العنوان: | High throughput screening identifies SOX2 as a super pioneer factor that inhibits DNA methylation maintenance at its binding sites |
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| المؤلفون: | Vanzan, Ludovica, Soldati, Hadrien, Ythier, Victor, Anand, Santosh, Braun, Simon M. G., Francis, Nicole, Murr, Rabih |
| المصدر: | Nature Communications, Vol 12, Iss 1, Pp 1-18 (2021) Nature Communications Nature Communications, Vol. 12, No 1 (2021) P. 3337 |
| بيانات النشر: | Nature Portfolio, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | DNA Replication, Epigenomics, oct motifs, Science, Gene Expression, cooperative binding, ddc:616.07, embryonic stem-cells, Article, genome-wide, Mice, ddc:590, de-novo, Sf9 Cells, Animals, ddc:576.5, RNA, Small Interfering, DNA methylation, Binding Sites, SOXB1 Transcription Factors, fungi, factors shape, DNA, proteins, High-Throughput Screening Assays, Nucleosomes, DNA Demethylation, transcription factor-binding, chromatin, Octamer Transcription Factor-3, n-myc, Protein Binding, Transcription Factors |
| الوصف: | Binding of mammalian transcription factors (TFs) to regulatory regions is hindered by chromatin compaction and DNA methylation of their binding sites. Nevertheless, pioneer transcription factors (PFs), a distinct class of TFs, have the ability to access nucleosomal DNA, leading to nucleosome remodelling and enhanced chromatin accessibility. Whether PFs can bind to methylated sites and induce DNA demethylation is largely unknown. Using a highly parallelized approach to investigate PF ability to bind methylated DNA and induce DNA demethylation, we show that the interdependence between DNA methylation and TF binding is more complex than previously thought, even within a select group of TFs displaying pioneering activity; while some PFs do not affect the methylation status of their binding sites, we identified PFs that can protect DNA from methylation and others that can induce DNA demethylation at methylated binding sites. We call the latter super pioneer transcription factors (SPFs), as they are seemingly able to overcome several types of repressive epigenetic marks. Finally, while most SPFs induce TET-dependent active DNA demethylation, SOX2 binding leads to passive demethylation, an activity enhanced by the co-binding of OCT4. This finding suggests that SPFs could interfere with epigenetic memory during DNA replication. The functional relevance of epigenetic modifications on transcription regulation has been an important question since their discovery. Here, the authors investigate the effect of DNA methylation on Pioneer Transcription Factor (PF) binding and distinguish between PFs that protect their binding sites from methylation and those that bind to methylated DNA and induce DNA demethylation. |
| اللغة: | English |
| تدمد: | 2041-1723 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::fb539c790f2e6e940fc9eae7d68db2a3Test https://doaj.org/article/c700bba4738648ac97b97852cfa493ccTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.pmid.dedup....fb539c790f2e6e940fc9eae7d68db2a3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20411723 |
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